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Biotechnology Div 1

Biotechnology Competition Division One 2010

QuestionAnswer
Biotechnology The use of living organisms or other biological systems in the manufacture of drugs or other products or for environmentalmanagement, as in waste recycling
Buffer Solutions that have the capacity withstand the addition of small amounts of protons and/or hydroxide ions, or undergo dilution, without a dramatic change in pH.
DNA deoxyribonucleic acid
Column Chromatography The separation of mixtures into their constituents by preferential adsorption by a solid, as a column of silica
Size Exclusion Chromatography (gel filtration) Chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column
Gel Filtration (size exclusion) Chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column
Elution buffer In column chromatography, it is put in the column to basically cleanse and lubricate. Generally, it helps to wash out any left-over proteins from a previous experiment. It can also help to separate the fractions that are collected.
Equilibrium buffer The process of exposing a liquid to a gas that is at a certain partial pressure until the partial pressures of the gas inside and outside the liquid are equal.
Mobile Phase In liquid chromatography, the phase that is moving in the bed, including the fraction of the sample held by this phase. The carrier gas in a gas chromatography procedure.
Stationary Phase In chromatography, the nonmobile phase contained in the chromatographic bed. 

Pipettor Laboratory instrument used to transport a measured volume of liquid.
Protein any of numerous, highly varied organic molecules constituting a large portion of the mass of every life form and necessary in the diet of all animals and other nonphotosynthesizing organisms
Epithelial Cells Form the epithelial tissue that lines both the inner and outer surfaces of the body; serve a protective function and also aid in absorption and secretion.
Protein Primary Structure Consists of the order in which amino acids are bonded to one another by peptide bonds.
Protein Secondary Structure Involves the way that that chain of amino acids either twists or folds back upon itself to form either alpha helical, beta sheet or a variety of other possible arrangements.
Protein Tertiary Structure Secondary structure in turn folds back and twists and bonds to itself in a three-dimensional manner
Precipitation the precipitating of a substance from a solution
Solubility the quality or property of being soluble; relative capability of being dissolved
Aqueous Phase The water portion of a system consisting of two liquid phases, one that is primarily water and a second that is a liquid immiscible with water
Ethanol Organic compound.Produced by fermentation, it is the intoxicating ingredient in alcoholic beverages. Ethanol for industrial purposes is made by either fermentation or chemical synthesis, then purified by distillation
Hydrophilic/Hydrophobi Having a strong affinity for water
Diagnostic Serving to identify or characterize; being a precise indication
Created by: is2903