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CH 6 Microbiology

Microbial Growth

TermDefinition
Psychrophiles cold-loving microbes Max growth temp- 20 degrees celsius
Mesophiles moderate-temperature-loving microbes optimum growth temp- 25-40 degrees celsius Most spoilage and pathogenic microbes
Strict Psychrophiles optimum growth 15 degrees celsius
Psychrotrophs optimum temperature of 20- 30 degrees celsius most common in cases of low-temperature food spoilage
optimum growth temperature temperature at which an organism grow best
minimum and maximum growth temperatures provide the range of temperatures that an organism can survive in
Thermophiles heat-loving microbes optimum growth temperature 50-60 degrees celsius minimum growth temperature 40 degrees celsius
hyperthermophiles optimum growth temperature of 80 degrees celsius or higher some archaea
acidophiles bacteria and other microbes that prefer acidic conditions
obligate halophiles require high salt concentrations and lower water concentrations to function
facultative halophiles prefer normal water concentrations max 98% min 85%
obligate aerobes require oxygen to survive
facultative anaerobes can survive without oxygen but grow more in the presence of oxygen
Microaerophiles require low amounts of oxygen to survive higher concentrations are toxic
Aerotolerant anaerobes survive equally well with or without oxygen
obligate anaerobes require the absence of oxygen
biofilms communities of microbes in thin layers on various surfaces
culture medium nutrient material used for microbe growth
inoculum microbes added to a culture medium to initiate growth
culture group of microbes growing in/on a culture medium
sterile culture medium (or other substance) without any microbes present
agar polysaccharide derived from algae used to generate solid culture media
chemically defined medium culture medium where the exact chemical composition is known
minimal medium a type of chemically defined medium with the minimal amount of nutrients for microbe survival
selective medium culture medium designed to optimize growth for specific organisms
differential medium culture medium designed to allow easy identification of specific microbial cultures
complex medium culture medium without an exact chemical composition
lag phase occurs shortly after the bacterial inoculum is added to the culture medium
exponential growth phase occurs when the bacteria start growing rapidly
stationary phase occurs when environmental conditions slow down bacterial growth
death phase occurs when cell deaths outnumber new cells being formed
generation time (of a culture) time required for the live cell number in the culture to double
plate counts number of colonies formed on a plate
serial dilutions (or repeated dilutions of a culture) often necessary to reduce the number of microbes to a measurable number
direct microscopic count quantitative test and used to enumerate the number of bacterial clumps or somatic cells present in milk
most probable number method statistical process of estimating microbial population
turbidity cloudiness of the culture medium
metabolic activity measurement of nutrient consumption or waste product formation
dry weight removal and drying of microbes and measurement of mass
Created by: kbarcenas9
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