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CH 6 Microbiology
Microbial Growth
| Term | Definition |
|---|---|
| Psychrophiles | cold-loving microbes Max growth temp- 20 degrees celsius |
| Mesophiles | moderate-temperature-loving microbes optimum growth temp- 25-40 degrees celsius Most spoilage and pathogenic microbes |
| Strict Psychrophiles | optimum growth 15 degrees celsius |
| Psychrotrophs | optimum temperature of 20- 30 degrees celsius most common in cases of low-temperature food spoilage |
| optimum growth temperature | temperature at which an organism grow best |
| minimum and maximum growth temperatures | provide the range of temperatures that an organism can survive in |
| Thermophiles | heat-loving microbes optimum growth temperature 50-60 degrees celsius minimum growth temperature 40 degrees celsius |
| hyperthermophiles | optimum growth temperature of 80 degrees celsius or higher some archaea |
| acidophiles | bacteria and other microbes that prefer acidic conditions |
| obligate halophiles | require high salt concentrations and lower water concentrations to function |
| facultative halophiles | prefer normal water concentrations max 98% min 85% |
| obligate aerobes | require oxygen to survive |
| facultative anaerobes | can survive without oxygen but grow more in the presence of oxygen |
| Microaerophiles | require low amounts of oxygen to survive higher concentrations are toxic |
| Aerotolerant anaerobes | survive equally well with or without oxygen |
| obligate anaerobes | require the absence of oxygen |
| biofilms | communities of microbes in thin layers on various surfaces |
| culture medium | nutrient material used for microbe growth |
| inoculum | microbes added to a culture medium to initiate growth |
| culture | group of microbes growing in/on a culture medium |
| sterile | culture medium (or other substance) without any microbes present |
| agar | polysaccharide derived from algae used to generate solid culture media |
| chemically defined medium | culture medium where the exact chemical composition is known |
| minimal medium | a type of chemically defined medium with the minimal amount of nutrients for microbe survival |
| selective medium | culture medium designed to optimize growth for specific organisms |
| differential medium | culture medium designed to allow easy identification of specific microbial cultures |
| complex medium | culture medium without an exact chemical composition |
| lag phase | occurs shortly after the bacterial inoculum is added to the culture medium |
| exponential growth phase | occurs when the bacteria start growing rapidly |
| stationary phase | occurs when environmental conditions slow down bacterial growth |
| death phase | occurs when cell deaths outnumber new cells being formed |
| generation time (of a culture) | time required for the live cell number in the culture to double |
| plate counts | number of colonies formed on a plate |
| serial dilutions (or repeated dilutions of a culture) | often necessary to reduce the number of microbes to a measurable number |
| direct microscopic count | quantitative test and used to enumerate the number of bacterial clumps or somatic cells present in milk |
| most probable number method | statistical process of estimating microbial population |
| turbidity | cloudiness of the culture medium |
| metabolic activity | measurement of nutrient consumption or waste product formation |
| dry weight | removal and drying of microbes and measurement of mass |