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micro ch8

Microbial Metabolism

QuestionAnswer
Metabolism sum of all chemical reactions within a living organism
Catabolism Breaks down large molecules, breaks chemical bonds, releases potential energy used for ATP
Hydrolysis reactions break polymers back down into monomers
Anabolism builds large organic molecules, Biosynthesis, requires ATP
How are Polymers Built? built from monomers by condensation or dehydration synthesis reactions
Simple enzymes consist of proteins alone
Coenzymes organic helper molecules. Accept ot donate atoms or electrons, act as energy carrier, derived from vitamins,
Cofactor inorganic helper molecules, Fe,Cu,Mg,Zn,Ca,Co
Exoenzyme function when released outside of cell
Endoenzymes remain in cell and function there
ATP Energy storage molecule consists of nucleotide and contains 2 high energy unstable bonds
Oxidation removes electrons, produces energy
Reduction gain of electrons
Dehydrogenation removes h+ and an electron
Biological Oxidations a. organic molecule is oxidized b) 2 (-) and 2 H+ proton transferred to coenzyme, nad accepts 1h, nadh, other h+ released
Generation of ATP Energy release by redox reactions, Phosphorylation- adds a phosphate to a compound
Aerobic respiration final electron acceptor is oxygen
Anaerobic respiration final electron acceptor is inorganic ion other than oxygen
Pyruvic Acid central metabolite- can be reduced into varius products by process of fermentation or converted to acetyl-CoA to enter krebs cycle
Glycolysis ATP production 4ATP produced- 2 used.Net Yield 2 ATP, 2 NAD+2 NADH 6ATP
ATP production in Conversion of pyruvic acid to acetyl CoA 2NAD+  2NADH , 6ATP
Krebs Cycle 6NAD+  6NADH, 18ATP, 2FAD+ 2FADH, 4 ATP, 2 ATP 2ATP
Prokaryotes yield how much ATP 38 ATP
Eukaryotes Yield how much ATP 36 ATP (2 are spent to take reactions from cytoplasm in to mitochondria
Lactic Acid Fermentation substrate- glucose, end product- lactic acid, Organisms- Lactobacillus, Stretococcus, results in food spoilage, dental caries, yogurt, sauerkraut, pickles
Alcohol Fermentation Substrate- glucose, end product- ethanol and CO2, organisms- Saccharomyces cervesiae (brewers yeast) baking bread, wine, beer, liquors
Industrial Uses for Fermentation Acetone, Acetic Acid (vinegar) ,Organic acids, Alcohols
Biochemical Testing test medium- contains protein an single source carb for energy, PH indicator- changes color if acid is produced, Durham tubes- small glass tube will trap gas to detect gas production
Lipid Catabolism Lipids glycerol and fatty acids, glycerol is funneled into the krebs cycle, fatty acids are broken down by Beta oxidation cycle into acetyl CoA, Some bacteria degrade lipids like petroleum
Protein catabolism proteins  amino acids, (20 common), acids transported into cell, Deamination, Decarboxylation and dehydrogenation
Deamination removes an amino group from an amino acid, converts it to ammonia, the rest of amino acid goes to krebs cycle
Decarboxylation and dehydrogenation removes a carboxyl group from an amino acid, rest of the amino acid feeds into krebs cycle
Biosynthesis- Metabolic Pathways are integrated catabolic pathways release energy n produce intermediates and end products can b used for anabolic pathways-synthesize building blocks synthesize macromolecules
Created by: ktlapp
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