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micro ch8
Microbial Metabolism
| Question | Answer |
|---|---|
| Metabolism | sum of all chemical reactions within a living organism |
| Catabolism | Breaks down large molecules, breaks chemical bonds, releases potential energy used for ATP |
| Hydrolysis reactions | break polymers back down into monomers |
| Anabolism | builds large organic molecules, Biosynthesis, requires ATP |
| How are Polymers Built? | built from monomers by condensation or dehydration synthesis reactions |
| Simple enzymes | consist of proteins alone |
| Coenzymes | organic helper molecules. Accept ot donate atoms or electrons, act as energy carrier, derived from vitamins, |
| Cofactor | inorganic helper molecules, Fe,Cu,Mg,Zn,Ca,Co |
| Exoenzyme | function when released outside of cell |
| Endoenzymes | remain in cell and function there |
| ATP | Energy storage molecule consists of nucleotide and contains 2 high energy unstable bonds |
| Oxidation | removes electrons, produces energy |
| Reduction | gain of electrons |
| Dehydrogenation | removes h+ and an electron |
| Biological Oxidations | a. organic molecule is oxidized b) 2 (-) and 2 H+ proton transferred to coenzyme, nad accepts 1h, nadh, other h+ released |
| Generation of ATP | Energy release by redox reactions, Phosphorylation- adds a phosphate to a compound |
| Aerobic respiration | final electron acceptor is oxygen |
| Anaerobic respiration | final electron acceptor is inorganic ion other than oxygen |
| Pyruvic Acid | central metabolite- can be reduced into varius products by process of fermentation or converted to acetyl-CoA to enter krebs cycle |
| Glycolysis ATP production | 4ATP produced- 2 used.Net Yield 2 ATP, 2 NAD+2 NADH 6ATP |
| ATP production in Conversion of pyruvic acid to acetyl CoA | 2NAD+ 2NADH , 6ATP |
| Krebs Cycle | 6NAD+ 6NADH, 18ATP, 2FAD+ 2FADH, 4 ATP, 2 ATP 2ATP |
| Prokaryotes yield how much ATP | 38 ATP |
| Eukaryotes Yield how much ATP | 36 ATP (2 are spent to take reactions from cytoplasm in to mitochondria |
| Lactic Acid Fermentation | substrate- glucose, end product- lactic acid, Organisms- Lactobacillus, Stretococcus, results in food spoilage, dental caries, yogurt, sauerkraut, pickles |
| Alcohol Fermentation | Substrate- glucose, end product- ethanol and CO2, organisms- Saccharomyces cervesiae (brewers yeast) baking bread, wine, beer, liquors |
| Industrial Uses for Fermentation | Acetone, Acetic Acid (vinegar) ,Organic acids, Alcohols |
| Biochemical Testing | test medium- contains protein an single source carb for energy, PH indicator- changes color if acid is produced, Durham tubes- small glass tube will trap gas to detect gas production |
| Lipid Catabolism | Lipids glycerol and fatty acids, glycerol is funneled into the krebs cycle, fatty acids are broken down by Beta oxidation cycle into acetyl CoA, Some bacteria degrade lipids like petroleum |
| Protein catabolism | proteins amino acids, (20 common), acids transported into cell, Deamination, Decarboxylation and dehydrogenation |
| Deamination | removes an amino group from an amino acid, converts it to ammonia, the rest of amino acid goes to krebs cycle |
| Decarboxylation and dehydrogenation | removes a carboxyl group from an amino acid, rest of the amino acid feeds into krebs cycle |
| Biosynthesis- Metabolic Pathways are integrated | catabolic pathways release energy n produce intermediates and end products can b used for anabolic pathways-synthesize building blocks synthesize macromolecules |