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6.1.1 Microscopy
about microscopes used to look at cells
| Question | Answer |
|---|---|
| cell | an organism’s basic unit of structure and function |
| the type of microscope you are likely to use in the laboratory | light microscope |
| the three important parameters in microscopy | magnification, resolution, and contrast |
| magnification | the ratio of an object’s image size to its real size |
| resolution | a measure of the clarity of the image; the minimum distance two points can be separated and still be distinguished as separate points |
| contrast | the difference in brightness between the light and dark areas of an image |
| Staining or labeling cell components to stand out visually are | methods for enhancing contrast |
| cell fractionation | takes cells apart and separates major organelles and other subcellular structures from one another |
| centrifuge | the piece of equipment used for cell fractionation |
| differential centrifugation | when the centrifuge spins the test tubes holding mixtures of disrupted cells at increasing speeds |
| In differential centrifugation, at lower speeds, the pellet consists of _______ components | larger |
| In differential centrifugation, at ______ speeds, the pellet consists of smaller components | higher |
| TEM stands for | transmission electron microscopy |
| SEM stands for | scanning electron microscopy |
| this type of microscopy shows a thin section of the specimen | TEM |
| this type of microscopy shows a 3D image of the surface of the specimen | SEM |
Created by:
zys
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