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First Aid Micro

Stain-Culture review

QuestionAnswer
What media is required for culture of H influenzas? Chocolate agar
What are the contents of the chocolate agar? Factors V(NAD+) and X (hematin)
Chocolate agar. Organism? H influenzae
What is the media used for N gonorrhoeae and N meningitidis? Thayer-Martin agar
Which organism is cultured with Thayer-Martin agar? N gonorrhoeae and N meningitidis
What component of Thayer-Martin agar inhibits growth of gram (+) organisms? Vancomycin
Which component of Thayer-Martin agar inhibits growth of gram (-) organisms? Trimethoprim and colistin
Which component of the Thayer-Martin gar inhibits growth of fungi in the culture? Nystatin
What are the 4 drugs contained in Thayer-Martin agar, which inhibit most pathogenic growth? Vancomycin, Trimethoprim, Colistin, and nystatin
What 2 media are used to growth B pertussis? 1. Bordet-Gengou agar 2. Regan-Lowe medium
What is the media content of Bordet-Gengou agar? Potato extract
What is the media content of Regan-Lowe medium? Charcoal, blood, and antibiotics
Which bug or pathogen is cultured by Bordet-Gengou agar? B pertussis
Which pathogen is cultured with Reagan-Lowe medium? B pertussis
What medium is used for isolation of C diphtheria? Tellurite agar, Loffer medium
Which pathogen is suspected in a culture containing Tellurite agar and Loffer medium? C diphtheriae
What are the main media used for isolation growth of M tuberculosis? Lowenstein-Jensen medium and Middlebrook medium
Media for M pneumoniae? Eaton agar
What bacteria uses Eaton agar? M pneumoniae
What is required ro Eaton agar to work? Cholesterol
What is the media used for Lactose-fermenting enteric? MacConkey agar
What color does the colonies produced by Lactose fermenting enteric in MacConkey agar turn? Pink
What is the specific media used by E. coli? Eosin-methylene blue (EMB) agar
What color do E coli colonies in EMB agar turn? Green metallic sheen
A unknown culture produces a green metallic sheen, what is the most likely bacteria growing? E coli
Media for Brucella, Francisella, Legionella, and Pasteurella? Charcoal yeast extract agar buffered with cysteine and iron
What is used to buffered charcoal year extract agar? Cysteine and iron
Which bacteria are cultured in Charcoal yeast extract? Brucella, Francisella, Legionella, Pasteurella
What "bug" is cultured in Sabouraud agar? Fungi
What is the main purpose of Selective media? Favors the growth of a particular organism while preventing growth of other organisms
Yields a color change in response to the metabolism of certain organisms Indicator (differential) media
Created by: rakomi
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