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Micro T1
Micro T1 intro material
| Question | Answer |
|---|---|
| Mycology | study of fungi |
| Phycology | study of algae and seaweed |
| protozoology | unicellular organisms |
| parasitology | unicellular and feed on a host |
| microbiology | study of any organism a microscope is needed to see |
| bacteriology | study of bacteria |
| virology | study of viruses |
| immunology | how the body reacts to microbes |
| serology | study of serum |
| serum | antibodies and antigens |
| how many microorganisms do we know of? | about 3,000 |
| how many microorganisms are known to be bad? | about 300 |
| estimate of total yet to be discovered... | possibly a million |
| areas of applied micobiology... | food and dairy, sanitation, bioremediation, industrial, soil and agriculture, plant pathology, medical |
| epidemology | trace origin of infection and control the spread |
| zoonoses | disease that can spread from animals to humans |
| leeuwunhoek | first viewing of microscopes in 1673 |
| when were microbes first viewed? | 1673 |
| significance of leeuwehoek | made people aware of microbes |
| pasteur | killed theory of spontaneous generation |
| spontaneous generation | the idea that life can spring from non-life |
| fermentation | anaerobic respiration |
| schwann | showed role of yeast in alcoholic fermentation |
| The father of microbiology | pasteur |
| pasteur | invented pasturization after discovering reason for spoilage in wine |
| germ theory of disease | the idea that microorganisms cause disease |
| % of disease caused by microbes? | 50% |
| Bassi | studied silk worm fungal diease |
| schonlein | studied human skin fungal disease |
| simmelweis | implemented hand washing to stop spread of child-birth fever |
| nosocomial infection | hospital acquired |
| pasture studied | pebrine disease in silk worms |
| lister | implemented aseptic methods into surgury (heat sterilize) |
| sterile means... | no living things |
| Koch | demonstrated that bacteria cause disease (worked with anthrax) |
| koch postulate 1 | extract organism from diseased animal |
| koch postulate 2 | identify organism |
| koch postulate 3 | inject healthy animal |
| koch postulate 4 | healthy gets disease |
| koch postulate 5 | match first disease cause to second disease cause |
| contrast | ability to define the edge |
| contrast enhanced by? | staining, lens quality |
| what is a vital stain? | a stain that can be used on living cells |
| resolution | the ability to see fine detail, distinguish between two points |
| brightfield | most common, need to stain |
| darkfield | better contrast than bright, no stain needed, good for Treponema pallidum |
| best resolution overall | electron microscope |
| best resolution of light microscopes | UV |
| Good resolution and contrast | flourescent |
| can view live organisms | phase contrast and DIC |
| live organisms internals | DIC |
| live organisms externals | phase contrast |
| uses one wavelength of light | confocal |
| what is biological uncertainty? | when the process of specimen preparation causes artifacts |
| resolution of regular light mocroscope | abouut 0.2 um |
| rosolution of UV | about 0.1 um |