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exam 1
biochem/genetic combo
| Question | Answer |
|---|---|
| which type of genetic transfer requires cell to cell contact | conjugation |
| which type of horizontal transfer is not a process evolved by bacteria to increase genetic diversity in a population | transformation, conjugation, transduction |
| adding which of the following did not result in transformation of an S. pnueamanie R strain | type s, type s + DNase, type s + RNase, type s + Protease |
| how often will a stop codon occur in a completly random DNA sequence | 1 every 14 codons |
| which of the following can be used to measure the distance between mutations within a gene | recombination frequency |
| if you add GDP and adenosine diphosphate to polynucleotide phosphorylase [PP] at a ratio of 70% GDP to 30% ADP, what percentage of the radioactivity in the resulting polypeptide will be lysine | 9 |
| which of the following statements about the Griffith transformation experiment with Type II and Type III S. pnueamanie is not true | a type II bacterium can mutate into a type II as a result of a single mutation |
| can a heat killed type IIIS bacteria kill an infected mouse | no |
| is the capsule of the heat killed type IIIS bacteria still intact. | yes |
| can a living Type IIR bacteria kill an infected mouse | no |
| wil heat killed type IIIS bacteria mixed with living Type IIR bacteria kill an infected mouse | yes |
| which of the following will produce negatively supercoiled DNA | DNA topoisomerase II added to double stranded DNA |
| What is the purpose of using a blender in the Hershey and Chase experiment to identify the identity of genetic material | displaces dsDNA that is attached to the surface of competent cells |
| what type of chromatin is associated with the liver specific gene in a muscle cell | facultative heterochromatin |
| what type of chromatin is associated with the rRNA gene | Euchromatin |
| what type of chromatin is associated with the centromere DNA | constitutive heterochromatin |
| in the Hershey Chase experiment where was S 35 primarily found | in the supernatant |
| in the Hershey Chase experiment where was P32 primarily found | Bacterial Pellet |
| the following figure shows the agarose gel result of the experiment proof of Markus Noll''s hypothesis | |
| draw RNA stem loop structures` | |
| explain Frederick Griffith experiment | |
| explain Oswald Avery experiment | |
| explain Hershey and chase experiment | |
| explain chargaff experiment | |
| explain watson, crick, and franklin experiment | |
| explain meselson and stahl experiment | |
| draw replication | |
| explain Noll experiment | |
| reproductive cycle of phage lambda | |
| reproductive cycle of HIV | |
| pulse chase activity | |
| dilutions | |
| a mutation to the polyadenylation sequence would affect which process first | transcription termination |
| a mutation to the Topoisomerase II [DNA gyrase] would affect which process first | DNA Replication Elongation, Topoisomerase II is used during elongation to alleviate supercoiling ahead of repliccation fork |
| a mutation to the DnaA box would affect which process first | DNA Replication Initiation |
| a mutation to the primase would affect which process first | DNA Replication Initiation |
| a mutation to theTUS protein would affect which process first | DNA Replication termination |
| a mutation to the DnaB [helicase] would affect which process first | DNA Replication Initiation |
| Would a mutation in dam gene [Dam methylase] that incsreases its catalytic activity; increase, decrease, or have no effect on the rate of DNA replication initiation | increase |
| Would a introduction of dnaA gene [DnaA protein] on a 50 copy per cell plasmid ; increase, decrease, or have no effect on the rate of DNA replication initiation | increase |
| Would a mutation in subunit of DNA pol III that speeds up addition of nucleotides to 3'end of new strand; increase, decrease, or have no effect on the rate of DNA replication initiation | no effect |
| Would a introduction of GATC sequence on a 50 copy per cell plasmid ; increase, decrease, or have no effect on the rate of DNA replication initiation | decrease |
| what would result from an E coli strain with a mutation that eliminates the 3' to 5' exonuclease activity of DNA Polymerase III | increased mutation rate |
| Which of the following would be a slow stop mutant in a temperature shift DNA replication experiment | DnaA and DnaC |
| parts of the amino-acyl tRNA synthase enzyme | amino acid binding site, ATP binding site, ribosome binding site, tRNA binding site |
| parts of the replisome in ecoli | primase, Polymerase III, Helicase, |
| briefly describe how you would isolate temperature sensitive mutations in genes that are required for Ecoli viability. | |
| human telomeres consist of many repeats | |
| Draw a Michaelis-Menten plot for an enzyme Please indicate the Vmax and Km on the graph you draw. Sketch on this plot what would happen if this enzyme formed a tetramer that cooperatively bound four substrate molecules. | |
| identify the class (carbohydrate, peptide, fatty acid, nucleic acid base, terpene, etc.) to which each of the following molecules belongs | |
| Which Ramachandran plot is the plot for glycine and which is the plot for proline | |
| Which sequences are likely to be found in (i) amphipathic -helices? | |
| Identify three different functional groups in the molecule below | |
| Match each amino acid sidechain or prosthetic group (atom or molecule attached to a protein that is not a proteinogenic amino acid) with a corresponding functional group/chemical property. | |
| Which equation would you be most likely to use to calculate the pH in the given situation – the equilibrium equation for Ka, the Henderson-Hasselbalch equation or merely the definition of pH? | |
| Dissolving a solid/liquid solute in a liquid solvent can be conceptualized as occurring via the following mechanism: | |
| classify each of the following proteins as globular or fibrous, and if fibrous, indicate the primary type of secondary structure in the protein | |
| Indicate the name and one letter code for the amino acid best fitting the given description | |
| Which of the following are true statements about the enzyme Thrombin (indicate all that are true)? | |
| A biochemist separates 3 proteins using a cation exchange column (column | |
| Weak transient bonds involved in DNA structure | Hydrogen bonds, ionic bonds, hydrophobic van der wals |
Created by:
Virajasaur
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