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Micro Lab 15-17
Gram, Spore, & AF Staining
| Q: Ex 15-17 | A: Ex 15-17 |
|---|---|
| what color would you expect S. aureus to be if the iodine step were omitted in the Grame-staining procedure? | It would still be purple, maybe just a lighter color of purple because iodine serves to intensify the first color not make it stick |
| what structures of the bacterial vell appears to play the most important role in determining whether an organism is G+? | The peptidodoglycan layer of the cell wall |
| Why would methylene blue not work just as well as safranin for ocunterstaining in the G-staining procedure? | Methylen blue would not provide as strong of a contrast to the crystal violet as the safranin does. |
| Why are endospores so difficult to stain? | The exosporium layer surrounding the endospore makes it difficult to stain. |
| How do the following two genera of spore-formers differ physiologically? Bacillus vs. Clostridium | Bacillus is an aerobic form of spore-former while Clostridium is an anaerobic form of spore-former |
| How do you differentiate S. aureus and M.B. catarrhalis from each other on the basis of morphological characteristics? | (blank) |
| Are the acid-fast mycobacteria G+ or G-? | (blank) |
| For what two diseases is AF staining of paramount importance? | a. Mycobacterium leprae (Leprosy) b. Mycobacterium tuberculosis (tuberculosis) |
| What two genuses of bacteria have a waxy material in their cell walls? | a. Mycobacterium b. Nocardia |
| what is the waxy material in the cell walls of Mycobacterium and Nocardia? | mycolic acid |
| Are the AF mycobacteria G+ or G- | (blank) |
| Why is it desirable to combine S. aureus with AF organisms such as M. smegmatis when applying an AF staining technique? | (blank) |
| What is a primary stain? | (blank) |
| What is a secondary stain? | (blank) |
| What color is a G+ organism? | purple |
| what color is a G- organism? | pink |
| what is a mordant? | a chemical that complexes with the primary stain to enhance the primary stain |
| What is peptidoglycan? | a thick cell wall that is a protein complex and carbohydrate; G+ organisms have a thick wall of it |
| What is S. aureus? | colonizes mainly the nasal passages, but it may be found regularly in most other anatomical locales; causes pneumonia, mastitis, phlebitis, meningitis, and urinary tract infections; yields principally lactic acid; catalase-positive and oxidase-negative |
| What does S. aureus look like? | fairly large yellow colony on rich medium |
Created by:
rrlong
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