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Cell bio-

Protein Synthesis

QuestionAnswer
Myotonic dystrophy gene silencing is the result of splicing alterations
what is the difference between charged and uncharged tRNAs charged tRNA has a.a
tRNA binding is parallel and semi-complementary F : its antiparallel and complementary
mRNA is read __ direction and paired by a tRNA in the __ direction. 5'- 3' and 3' to 5'
an enzyme that attaches an amino acid to its corresponding tRNA with a covalent bond aminoacyl-tRNA synthetase : requires ATP
aminoacyl-tRNA sits at A site
peptidyl tRNA sits at P site
wobble hypothesis A tRNA can recognize more than one codon for a specific amino acid.
initiation codon AUG
translation factors that facilitate initiation IF-2 in prokaryotes and eIF2 in eukaryotes
on which organism is the initator tRNA binds to an N -formulated methioine prokaryotes , not in eukaryotes
amino acid is added to what end of the growing polypeptide chain amino acid
Ribosome moves from___ to __ end of mRNA. 5’-end to 3’-
Elongation factors ----- facilitate the delivery of the aminoacyl-tRNA to the next corresponding codon on the mRNA EF-Tu and EF-Ts they require GTP
the peptide bond is catalyzed by peptidyl transferase ; a reaction facilitated by 23s rRNA. which is a ribozyme
In order for the ribosome to advance 3 nucleotide down on the mRNA , what is required EF-G and GTP
elongation :: The charged tRNA is released and The peptidyl-tRNA moves from the A site to the P site. first is false , second it true
termination release factors RF-1 and Rf-2 ; RF-3 binds GTP and stimulates the activity of RF-1 and RF-2
high levels could inhibit mitochondrial protein synthesis chloramphenicol
an enzyme that cleave a portion of the protein to activate it Endoproteases
in post-translational hydroxylation what a.a are hydroxylated proline and lysine
example of diseases cause by tandem repeats huntington ( extraglutamine) fragile X and myotonic dystrophy
Created by: jemalk
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