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pyrimidines thymine and cytosine
purines adenine and guanine
semi conservative replication DNA molecule unwinds by a gyrase enzyme, then unzips by DNA helicase then DNA polymerase catalyses the addition of new nucleotide bases. Leading strand is synthesised continuously where as the lagging strand is in fragments later joined by ligase enzymes
Universal almost in all living organisms the same triplet of dna bases codes for the same amino acid
degenerate for almost all amino acids there is more than one base triplet
non overlapping read starting from a fixed point in groups of three bases
Transcription gene unwinds and unzips, hydrogen bonds break, enzyme RNA polymerase catalyses formation of temporary hydrogen bonds with RNA nucleotides. mRNA is the coding strand.
Translation tRNA molecules bring amino acids and bind the anticodon to the complementary codon on the mRNA molecule. As the ribosome moves along the length of the mRNA peptide bonds form between amino acids that the coding strand reads. stop codon finished strand.
Purification of DNA macerate tissue by adding strong detergent, then ethanol, so DNA precipitates out of solution. further purify by removal of unwanted salts then concentrate.
Created by: katieappleton