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Microscopy
| Question | Answer |
|---|---|
| Magnification definition | describes how much bigger an image appears compared with the original object |
| Resolution definition | the ability of an optical instrument to see or produce an image that shows fine detail clearly |
| whats the range of magnification for optical microscopes? | up to x1500-x2000 |
| whats the limit of wavelength? | objects closer than 200nm(0.2um) will appear as one object |
| total magnification=? | magnifying power of objective lens x magnifying power of eyepiece lens |
| What do laser scanning microscopes use to produce an image? | Use laser light to produce high resolution and contrast |
| What type of specimens can they view? | whole, living specimens |
| What type of image does a transmission electron microscope produce? | 2D black and white image |
| Magnification for TEMs? | up to x2,000,000 |
| What type of image does a scanning electron microscope produce? | 3D black and white image , but adds false colour |
| magnification for SEMs? | x15-x200,000 |
| what type of specimens can SEMs use? | Dead as placed in vacuum and coated in fine metal |
| what type of specimen can TEMs use ? | Dead as chemically fixed and dehydrated |
| what is the stain for chromosomes? | acetic orcein |
| What is the stain for cytoplasm? | eosin |
| what is the stain for lipids? | Sudan red |
| what is the stain for plants? | iodine in potassium iodide solution, stains cell walls yellow, and starch granules blue/black |
| magnification=? | image/actual |
Created by:
katieappleton
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