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Microscopy

QuestionAnswer
Magnification definition describes how much bigger an image appears compared with the original object
Resolution definition the ability of an optical instrument to see or produce an image that shows fine detail clearly
whats the range of magnification for optical microscopes? up to x1500-x2000
whats the limit of wavelength? objects closer than 200nm(0.2um) will appear as one object
total magnification=? magnifying power of objective lens x magnifying power of eyepiece lens
What do laser scanning microscopes use to produce an image? Use laser light to produce high resolution and contrast
What type of specimens can they view? whole, living specimens
What type of image does a transmission electron microscope produce? 2D black and white image
Magnification for TEMs? up to x2,000,000
What type of image does a scanning electron microscope produce? 3D black and white image , but adds false colour
magnification for SEMs? x15-x200,000
what type of specimens can SEMs use? Dead as placed in vacuum and coated in fine metal
what type of specimen can TEMs use ? Dead as chemically fixed and dehydrated
what is the stain for chromosomes? acetic orcein
What is the stain for cytoplasm? eosin
what is the stain for lipids? Sudan red
what is the stain for plants? iodine in potassium iodide solution, stains cell walls yellow, and starch granules blue/black
magnification=? image/actual
Created by: katieappleton