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Biology Chapter 1
Activities for Microscopes MCB2010
| Question | Answer |
|---|---|
| What is the genus of bacteria that the Acid Fast stain is designed to be used to detect? | Mycobacteria |
| _____ media is one which suppresses growth of some microbes while allowed growthing of desired microbes. | Selective |
| _____ dyes have a positively charged colored ion. | Basic |
| What is the primary stain in the Acid Fast Stain? | Carbolfuschin |
| MacConkey agar selects FOR gram _____ bacteria. | Negative |
| What is the counterstain in the Acid Fast Stain? | Methylene Blue |
| If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the background around the cell be? | Gray |
| The color of the endospore after the endospore stain procedure shown in your notes. | Green |
| What is the mordant used in the gram stain procedure? | Iodine |
| The color of the vegetative cell in the endospore stain. | Pink |
| Lactose fermenters appear _____ on MacConkey agar. | Pink |
| The Acid Fast Stain is a ______ stain. | Differential |
| Non acid fast organisms appear what color after the Acid Fast stain procedure? | Blue |
| Non-lactose fermenters appear _____ on MacConkey agar. | Clear |
| _____ dyes have a negatively charged colored ion. | Acidic |
| Specialized, dormant structures that some types of bacteria can form under adverse conditions are known as ______. | Endospores |
| Media wherein the exact amount of each chemical is known. | Chemically Defined |
| Gram positive (+) cells appear what color after the gram staining procedure? | Purple |
| Acid fast organisms appear what color after the Acid Fast stain procedure? | Pink |
| The primary stain used in the endospore stain. | Malachite Green |
| Mycobacteria are stained with the acid fast procedure because of the _____ substance in their cell walls. | Waxy |
| What is the counterstain used in the gram stain? | Safranin |
| What is used as a mordant in the flagellar stain? | Carbolfuschin |
| A complex polysaccharide derived from seaweed which is added to nutrients to create a solid media. | Agar |
| _____ and _____ are examples of acidic dyes. | Nigrosin, India Ink |
| This type of media is used to grow anaerobes because it is a reducing media which depletes the oxygen in the media. | Thioglycolate |
| The surface of a bacterial cell is normally ______ charged. | Negatively |
| What is the decolorizing agent in the gram stain? | Alcohol |
| A _____ enhances the stain's affinity for the cell. | Mordant |
| Gram negative (-) cells appear what color after the gram staining procedure? | Pink |
| With the 10X ocular lens in place, what would be the total magnification if the 10X objective lens were used? | 100 |
| What is the primary stain used in the gram stain? | Crystal Violet |
| The term used for nutrient agar which is allowed to harden at an angle. | Slant |
| Media which does not define exactly what nutrients are there. | Complex Media |
| If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the capsule be? | Clear |
| If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the bacterial cell itself be? | Purple |
| ______ is an example of a basic dye. | Crystal violet |
| _______ agar selects for gram negative bacteria and differentiates between lactose fermenters and non-lactose fermenters. | MacConkey |
| _____ media is one which facilitates distinguishing between types of microbes. | Differential |
| _____ is the ability of a microscope to distiniguish two points at a specified distance. | Resolution |
| With the 10X ocular lens in place, what would be the total magnification if the 40X objective lens were used? | 400 |
| The Gram Stain is a ______ stain. | Differential |
| The _____ is a measure of the velocity of light as it passes through a specimen. | Refactive index |
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mikec35-pbsc
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