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Biology Chapter 1

Activities for Microscopes MCB2010

QuestionAnswer
What is the genus of bacteria that the Acid Fast stain is designed to be used to detect? Mycobacteria
_____ media is one which suppresses growth of some microbes while allowed growthing of desired microbes. Selective
_____ dyes have a positively charged colored ion. Basic
What is the primary stain in the Acid Fast Stain? Carbolfuschin
MacConkey agar selects FOR gram _____ bacteria. Negative
What is the counterstain in the Acid Fast Stain? Methylene Blue
If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the background around the cell be? Gray
The color of the endospore after the endospore stain procedure shown in your notes. Green
What is the mordant used in the gram stain procedure? Iodine
The color of the vegetative cell in the endospore stain. Pink
Lactose fermenters appear _____ on MacConkey agar. Pink
The Acid Fast Stain is a ______ stain. Differential
Non acid fast organisms appear what color after the Acid Fast stain procedure? Blue
Non-lactose fermenters appear _____ on MacConkey agar. Clear
_____ dyes have a negatively charged colored ion. Acidic
Specialized, dormant structures that some types of bacteria can form under adverse conditions are known as ______. Endospores
Media wherein the exact amount of each chemical is known. Chemically Defined
Gram positive (+) cells appear what color after the gram staining procedure? Purple
Acid fast organisms appear what color after the Acid Fast stain procedure? Pink
The primary stain used in the endospore stain. Malachite Green
Mycobacteria are stained with the acid fast procedure because of the _____ substance in their cell walls. Waxy
What is the counterstain used in the gram stain? Safranin
What is used as a mordant in the flagellar stain? Carbolfuschin
A complex polysaccharide derived from seaweed which is added to nutrients to create a solid media. Agar
_____ and _____ are examples of acidic dyes. Nigrosin, India Ink
This type of media is used to grow anaerobes because it is a reducing media which depletes the oxygen in the media. Thioglycolate
The surface of a bacterial cell is normally ______ charged. Negatively
What is the decolorizing agent in the gram stain? Alcohol
A _____ enhances the stain's affinity for the cell. Mordant
Gram negative (-) cells appear what color after the gram staining procedure? Pink
With the 10X ocular lens in place, what would be the total magnification if the 10X objective lens were used? 100
What is the primary stain used in the gram stain? Crystal Violet
The term used for nutrient agar which is allowed to harden at an angle. Slant
Media which does not define exactly what nutrients are there. Complex Media
If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the capsule be? Clear
If you used a basic dye such as crystal violet, and an acidic dye such as nigrosin on encapsulated bacteria, what would the appearance of the bacterial cell itself be? Purple
______ is an example of a basic dye. Crystal violet
_______ agar selects for gram negative bacteria and differentiates between lactose fermenters and non-lactose fermenters. MacConkey
_____ media is one which facilitates distinguishing between types of microbes. Differential
_____ is the ability of a microscope to distiniguish two points at a specified distance. Resolution
With the 10X ocular lens in place, what would be the total magnification if the 40X objective lens were used? 400
The Gram Stain is a ______ stain. Differential
The _____ is a measure of the velocity of light as it passes through a specimen. Refactive index
Created by: mikec35-pbsc
 

 



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