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enterics test
enterics, non fermenters, bioterrorism, MLT, microbiology
| Question | Answer |
|---|---|
| IMViC ++–– | E. Coli, Edwardsiella Shigella |
| IMViC ––++ | Klebsiella, Enterobacter |
| IMViC stands for | Indole, Methyl Red, Voges Proskauer, Citrate |
| A/A, ++–– | E. Coli |
| K/A, ++––, H2S+ | Edwardsiella |
| K/A, ++––, non motile, bacillary dysentery | Shigella |
| A/A, ––++, ODC–, non–motile | Klebsiella |
| K/A; A/A (late), ––++, ODC + | Enterobacter |
| K/A & DNase + | Serratia |
| K/A, PAD+, H2S++, & Urea++ | Proteus |
| K/A, PAD+, Urea+/–, & ODC– | Providencia |
| K/A, PAD+, Urea+,& ODC+ | Morganella |
| A/A or K/A, LAC +/–, H2S +/–, & LDC– | Citrobacter |
| O157:H7 | Sorbitol neg |
| K. pneumo & Proteus mirabilis | Indole – |
| K. oxytoca & Proteus vulgaris | indole + |
| red colonies | Serratia marcescens |
| delayed swarming | proteus vulgaris |
| motile at 25 C & can cause transfusion sepsis | Yersina enterocolitica |
| Media for Y. enterocolitica | CIN |
| CIN = | Cefsulodin, Irgasin, Novobiocin |
| watery diarrhea later bloody with pmns and mucus | shigellosis |
| watery diarrhea with high fever | typhoid fever |
| typhoid chronic carriers | lives in monocytes & seeded from gallbladder |
| Traveler's Diarrhea | ETEC |
| bloody diarrhea no PMNs | EHEC, STEC |
| which pathogen causes the bubonic plague | Yersinia pestis |
| 3rd most common isolate | proteus mirabilis |
| associated with fresh water and aquariums | Edwardsiella tarda |
| normal ranges for CSF | WBC 0–2/ul, Glucose 45–70 mg/dL, Protein 15–45 mg/dL |
| CSF ranges in Bacterial meningitis | WBCs >1000 (mostly segs), Glucose <40 mg/dL, Protein >100 mg/dL |
| <20k colonies on a urine culture | contaminate |
| >80k colonies on a urine culture | pathogen |
| if you use a 10 uL loop multiply by | 100 |
| if you use a 1 uL loop multiply by | 1000 |
| reject sputum if | no WBCs & TNTC squamous epithelial cells |
| difference between KIA and TSI | doesn't ferment sucrose |
| stool PIM for Salmonella and Shigella | HE, XLD, & SS |
| Shigella Anti –A | dysenteriae |
| Shigella Anti–B | flexneri |
| Shigella Anti–C | boydii |
| Shigella Anti–D | sonnei |
| Type of Antigens used to type Shigella | Somatic O |
| O/F Fermenters | yellow in open and closed tube |
| O/F non–utilizers | green in open and closed tube |
| O/F oxidizers | green in both tubes & yellow at the top of open tube |
| flat, spreading, feathered edge, green tinge, grape odor | Pseudomonas aeruginosa |
| pigments produced by P. aeruginosa | pyoverdin & pyocyanin |
| Pyoverdin | green, fluorescent |
| only produced by P. aeruginosa | pyocyanin |
| what antibiotics can be used to treat P. aeruginosa | 3rd and 4th gen cephalosporin |
| K/K, normal flora grows on SBA/MAC/EMB, oxidizer of CHOs oxidase –, catalase +, dead fish odor, cornflower blue | Acinetobacter baumannii |
| distinguish Acinetobacter from Moraxella or Neisseria | oxidase neg |
| beige (green with age) colonies on SBA, ammonia odor, NLF, oxidase –, Maltose + | Stenotrophomonas maltophilia |
| diffuse yellow colonies on SBA, NLF, slow/weak oxidase + | Burkholderia cepacia |
| yellow/beige on SBA, oxidase +, non motile, indole +, causes nosocomial meningitis and septicemia | Elizabethkingia meningoseptica |
| oxidase + with a fruity odor | Alcaligenes faecalis |
| what organism may be purple pigmented and therefore can't test for oxidase or indole | Chromobacterium violaceum |
| two species of Burkholderia genus that are agents of bioterrorism | mallei & pseudomallei |
| rice water stool | Vibrio cholerae |
| halophilic vibrio | parahaemolyticus & vulnificus |
| non–halophilic vibrio | cholera & mimicus |
| el tor serovars of Vibrio cholerae | can agglutinate chick RBC |
| classical serovars of Vibrio cholerae | can not agglutinate chick RBCs |
| distinguish vibrio from enterics | oxidase pos |
| describe vibrio cholerae on TCBS. What CHO does it ferment | yellow, sucrose |
| how is pleisomonas best distinguished form enterics | grows on CIN no bullseye |
| motility of campylobacter | darting |
| colony appearance of Campylobacter jejuni | beige, shiny, colonies that run along streaks. |
| gram stain feature of Campylobacter jejuni | gullwing |
| oxidase +, catalase +, hippurate +, naladixic acid Sensitive & Cephalothin resistant | Campylobacter jejuni |
| rapidly urease positive | H. pylori |
| spread by A/C or cooling towers | Legionella pneumophilia |
| human pathogen that can be sexually transmitted or be normal flora | Gardnerella vaginalis |
| special media for B. pertussis | Bordet/Gengou & Regan–Lowe |
| Bordet/Gengou ingredients | blood and potato glycerol |
| Regan–Lowe ingredients | horse, blood, and charcoal |
| infections caused by Pasteurella multocida | cat and dog bite wound, cat and dog scratch wound |
| grayish, beige with mousy odor on SBA, sick TSI reaction | Pasteurella multocida |
| special media for Legionella pneumophilia | BCYE or modified BCYE |
| Brucella in cows | abortus |
| Brucella in pigs | suis |
| Brucella in goats/sheep | melitensis |
| Brucella in dogs | canis |
| Media to recover Brucella | BCYE & Brucella Blood Agar |
| Media for Francisella tularensis | Cysteine blood Agar (CBA or BCA) & BCYE |
| how are Gardnerella vaginalis infections most often diagnosed? | clue cells |
| how does gardnerella differ from the other ellas on gram stain | gram variable |
| media for gardnerella | HBT & V agar |
| common ingredient on HBT and V agar | human blood |
| best media to isolate Bartonella | fresh CA or Horse, Rabbit, or Columbia blood in CO2. in high humidity keep 2 weeks |
| Bartonella that causes cat scratch disease | henselae |
| non motile bacillus species | anthracis |
| name the genus of aerobic sporeforming bacilli | bacillus |
| pulmonary anthrax | woolsorter's disease |
| cutaneous anthrax | malignant pustule or Black eschar |
| gram stain characteristic of anthrax | bamboo spores that don't swell the cell |
| SBA colony characteristic of anthrax | ground glass, medusa head |
| listeria monocytogenes motility | tumbling |
| L. monocytogenes appearance in motility media | umbrella |
| catalase +, BE +, hippurate +, camp test + (block) | Listeria monocytogenes |
| non motile, appears to have bottle brush motility in motility media | Erysipelothrix |
| diphtheroid means | side by side |
| characteristic GS of Corynebacterium | club shape, palasades, chinese letters, very pleomorphic |
| important for maintaining proper pH of the vagina | lactobacilli |
| normal skin flora | Corynebacterium jeikeium |
| C. diphtheriae appearance on Tinsdale agar | black or brown with brown halos and garlic odor |
| Selective ingredient in Tinsdales agar and Cystine Tellurite agar | Potassium tellurite |
| what are babes–ernst bodies | accumulations of polymerised polyphosphates |
| test used for Corynebacterium diphtheriae | ELEK |
| Media that enhance metachromatic granule production | Loeffler & PAI agar |
| rabbit fever, poor/no growth on SBA, slow growth on CA, Biohazard level III, potential agent of bioterrorism | Francisella tularensis |
Created by:
MamaDaBess
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