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Enzymes Ch9


What is metabolism The sum of all chemical reactions in a living cell
What is an enzyme Biological catalyst
From what biomolecule is an enzyme made from protein
What shape is an enzyme 3d globular folded shape
What is meant by saying that an enzyme is specific Only one particular enzyme will only work on one particular substrate
What is an immobilised enzyme Attached to each other or to an inert material
Give two physical methods of immobilised enzymes Trapped in a gel e.g. sodium alginate//adsorbed onto an inert material
Give two chemical methods of immobilising enzymes Bonded to each other//bonded to an inert material
Advantages of immobilised enzymes Recovered and reused//cheaper//more stable
Name the enzyme used in investigation Catalase
State the source of the enzyme Celery
State the substrate of the enzyme Hydrogen peroxide
State the products formed Water and oxygen
If varying temperature, how is the pH kept constant Using the same pH buffer
If varying temperature, how is the temperature varied Using a waterbath and a thermometer at different temperatures
If varying pH , how is the pH varied using different pH buffers
If varying pH how is the temperature kept constant Using a waterbath and a thermometer at 25 degrees
What is meant by optimum rate best activity it can work at
What is catalase optimium temperature 20-30 degrees
What is catalases optimium pH 7-9
How is the rate of enzyme activity measured Volume of foam produced/minute
What is a denatured enzyme Enzyme which has lost its 3d globular folded shape and can no longer function
Created by: eimeardoyle2014
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