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Bacillus & Nocardia

from Blood Cultures

QuestionAnswer
Which Bacillus species is used to test the autoclave? Bacillus stearothermophilus
What are the three manifestations of Bacillus anthracis? 1) Cutaneous anthrax, 2) Pulmonary/Inhalation anthrax, 3) GI anthrax
What are the main features of each manifestation of B. anthracis? Cutaneous: skin involvement with necrotic area known as "black eschar" Pulmonary: "woolsorter's disease" from inhaling spores from goat hair/hides GI: ingestion of spores and bloody diarrhea
How do Bacillus colonies appear on BAP? Large, gray colonies taking the appearance of frosted/ground glass with curling (Medusa head-like) projections. Colonies are firmly attached to the agar and may appear like "beaten egg whites" when removed.
When receiving direct smears of pus, CSF, and sputum, which stain can be used to reveal Nocardia species? Kinyoun's stain
What special agar is associated with the recovery of Nocardia species? Sabouraud dextrose agar
What inclusions are associated with Nocardia? Sulfur granules
How would Nocardia appear on a Gram Stain? Pleomorphic, gram positive rods in branching, coccal, or beaded forms
Created by: rowleea