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Bacillus & Nocardia
from Blood Cultures
Question | Answer |
---|---|
Which Bacillus species is used to test the autoclave? | Bacillus stearothermophilus |
What are the three manifestations of Bacillus anthracis? | 1) Cutaneous anthrax, 2) Pulmonary/Inhalation anthrax, 3) GI anthrax |
What are the main features of each manifestation of B. anthracis? | Cutaneous: skin involvement with necrotic area known as "black eschar" Pulmonary: "woolsorter's disease" from inhaling spores from goat hair/hides GI: ingestion of spores and bloody diarrhea |
How do Bacillus colonies appear on BAP? | Large, gray colonies taking the appearance of frosted/ground glass with curling (Medusa head-like) projections. Colonies are firmly attached to the agar and may appear like "beaten egg whites" when removed. |
When receiving direct smears of pus, CSF, and sputum, which stain can be used to reveal Nocardia species? | Kinyoun's stain |
What special agar is associated with the recovery of Nocardia species? | Sabouraud dextrose agar |
What inclusions are associated with Nocardia? | Sulfur granules |
How would Nocardia appear on a Gram Stain? | Pleomorphic, gram positive rods in branching, coccal, or beaded forms |