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generic-chapter 6
| Question | Answer |
|---|---|
| what are the requirement of DNA replicaiton | initationextensionUnzipping/ UnwindingproofreadingTermination |
| What is origins of replication | where DNA duplication begins. |
| What is the replicaiton of DNA | semiconservative |
| what is prokaryotes and eukaryotes | prokaryotes have one origins of replication whereas eukaryotes have many |
| what is the difference between unidirectional replication and bidirectional replicaiton? | Unidirectional replicaiton require only one replication whereas bidirectional replicaiton require two replicaiton fork |
| what proteins are participate in DNA replicaiton? | helicasesingle-stranded DNA biding proteins (SSB proteins)gyraseRNA primerDNA ligaseDNA polymerase IIIDNA polymerase I |
| what is the leading strand replicaiton | one strand is continuously synthesized in the normal 5'- 3' direction |
| Why is the proofreading so important? | Because it has a function to correct the mistake when the DNA polymerase adds the wrong nucleotide |
| what it the function of RNA primase | initiates new strand synthesis |
| what is the function of gyrase | cleaves and swivels duplex to release mechanical stress of unwinding |
| what is the function of DNA ligase | joins okazali fragments on lagging strand |
| what is th function of helicase | unwinds DNA at the replication fork |
| what is the function of SSB proteins | bind and stabilize single-stranded DNA at replicaiton fork. |
| what is complementary sequences? | a nucleic acid sequence of bases that can form a double-stranded structured by matching base pairs.(G-T-A-C) |
| what is phosphodiester linkage | a bond between the 3' carbon of on sugar to the 5' phosphate of the adjacent sugar |
| chargaff's rule | states that the ratios of purines to pyrimidines and also o adenine to thymine and of guanine to cytosine were close to one |
| conservative replication | both parent strands would remain together, and two new synthesis strands would form a second double helix |
| semiconservative replicaiton | hydrogen bonds between the two stands break, two strands separated and each trand pair with the nw complementary nucleotide to replace the missing partner. |