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Microbio Gram Stains

Gram Positives, Gram negatives

Gram Negative is what color? pink
Gram positive is what color? purple
Bacteria is differentiated by three things shape, size, quantity
Gram stains are performed when bacteria is the reason for S&S
What kind of culture is performed when it is negative a Blood culture
Infections can be bacterial, viral, fungal, parasitic
different types of bacterial infections affect the _______________ ____________ for the patient. Treatment Plan
Gram stains are done using blood, tissue, stool, urine, sputum
Gram negative Pathogens trigger these S&S's fever, vasodilation, inflammation, shock, DIC (disseminated intravascular coagulation)
Neisseria 6 facts Cocci/diplococci, aerobic, oxidase positive, fastidious growth, susceptible to drying and strains have fimbrae, capsules & LOS
LOS Lipid A + sugar
Neisseria gonorrhoeae STD, epithelial cells of mucous membranes, secretion IGa protease, survive and multiply inside phagocytes,
gonorrhea resistant to what antibiotics? penicillin, tetracycline, erythromycin
N. meningitidis/meningitis respiratory microbiota, capsule, transmitted by respiratory droplets, most disease caused by A,B,C and W135
how many antigenic strains of meningitis? 13
life threatening if it enters the bloodstream or CVF
damage of Meningitis is caused by Lipid A release into extracellular space- causes fever, shock, vasodilation, inflammation
Facultative anaerobes Bacilli Enterobacteriaceae
6 oliforms of EnteroBacteria.. E coli, Klebsiella, Serratia, Enterobacter, Hafnia, Citrobacter
4 non-coliforms of EnteroBacteria.... proteus, morganella, providencia, edwardsiella
3 true pathogens salmonella, shigella, yersinia
Pasteurellaceae-2 pasteurella, haemophilus
Enterobacteria.. and Pasteure..can be differentiated by what kind of test? oxidase test
Enterobacteria...... enteric bacteria,coccobacilli/bacilli
Pathogenicity of Enterobacteria... Outer membrane exotoxins, Capsules, fimbrae, exotoxins, hemolysins iron bounding compounds, Type 111 secretion systen
Type 111 secretion cause ____________which is _______________ Virulence, the capability/relative ability of a microorganism to cause disease
Enterobacteria....type 111- inserted into ________ __________ and enables bacteria to insert ____________ directly into the ______ acting as a needle Host membrane, chemicals, host
Coliforms are Gram neg, aerobic and facultative anaerobic, rod shaped, and ferment lactose;
Found in intestines, soil and plants Cold forms
6 opportunistic Gram Neg pathogens are Escherichia, Klebsiella, Serratia, Enterobacter, Hafnia, Citrobacter
E. coli is the __________ common and _________ important Most, most
E Coli has antigens ____, ______, and ______ identity strains O, H, K
E Coli virulent strains are O157, 0111, H8, H7
Fimbriae A fringe or fringed border
Coliform that is adhesions, endotoxins, enterotoxins (gastroenteritis) E Coli
Coliform that is nonmotile, capsules, digestive and respiratory tracts Klebsiella
Klebsiella pneumaoniae Destroys alveoli, bloody sputum
Non coli forms Enterobacteria... Cannot ferment lactose
Non-Coliform Entero.. Proteus mirabillis Bacilli, Gram neg., facultative anaerobe,
Few polar flagella in liquid culture and petrichious flagella culture on agar plate culture Proteus mirabillis
Facultative The power of doing or not doing something
True pathogenic Enterobacteria...characteristics Salmonella, Shigella, Yersenia- all type 111 secretion systems
Salmonella how many strains 2500
Salmonella produces Hydrogen sulfide
Characteristics of salmonella Gram neg, motile,
Motile Movement
Created by: gmtassa