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Microbio Gram Stains
Gram Positives, Gram negatives
Question | Answer |
---|---|
Gram Negative is what color? | pink |
Gram positive is what color? | purple |
Bacteria is differentiated by three things | shape, size, quantity |
Gram stains are performed when | bacteria is the reason for S&S |
What kind of culture is performed when it is negative | a Blood culture |
Infections can be | bacterial, viral, fungal, parasitic |
different types of bacterial infections affect the _______________ ____________ for the patient. | Treatment Plan |
Gram stains are done using | blood, tissue, stool, urine, sputum |
Gram negative Pathogens trigger these S&S's | fever, vasodilation, inflammation, shock, DIC (disseminated intravascular coagulation) |
Neisseria 6 facts | Cocci/diplococci, aerobic, oxidase positive, fastidious growth, susceptible to drying and strains have fimbrae, capsules & LOS |
LOS | Lipid A + sugar |
Neisseria gonorrhoeae | STD, epithelial cells of mucous membranes, secretion IGa protease, survive and multiply inside phagocytes, |
gonorrhea resistant to what antibiotics? | penicillin, tetracycline, erythromycin |
N. meningitidis/meningitis | respiratory microbiota, capsule, transmitted by respiratory droplets, most disease caused by A,B,C and W135 |
how many antigenic strains of meningitis? | 13 |
life threatening if it enters the | bloodstream or CVF |
damage of Meningitis is caused by | Lipid A release into extracellular space- causes fever, shock, vasodilation, inflammation |
Facultative anaerobes Bacilli | Enterobacteriaceae |
6 oliforms of EnteroBacteria.. | E coli, Klebsiella, Serratia, Enterobacter, Hafnia, Citrobacter |
4 non-coliforms of EnteroBacteria.... | proteus, morganella, providencia, edwardsiella |
3 true pathogens | salmonella, shigella, yersinia |
Pasteurellaceae-2 | pasteurella, haemophilus |
Enterobacteria.. and Pasteure..can be differentiated by what kind of test? | oxidase test |
Enterobacteria...... | enteric bacteria,coccobacilli/bacilli |
Pathogenicity of Enterobacteria... | Outer membrane exotoxins, Capsules, fimbrae, exotoxins, hemolysins iron bounding compounds, Type 111 secretion systen |
Type 111 secretion cause ____________which is _______________ | Virulence, the capability/relative ability of a microorganism to cause disease |
Enterobacteria....type 111- inserted into ________ __________ and enables bacteria to insert ____________ directly into the ______ acting as a needle | Host membrane, chemicals, host |
Coliforms are | Gram neg, aerobic and facultative anaerobic, rod shaped, and ferment lactose; |
Found in intestines, soil and plants | Cold forms |
6 opportunistic Gram Neg pathogens are | Escherichia, Klebsiella, Serratia, Enterobacter, Hafnia, Citrobacter |
E. coli is the __________ common and _________ important | Most, most |
E Coli has antigens ____, ______, and ______ identity strains | O, H, K |
E Coli virulent strains are | O157, 0111, H8, H7 |
Fimbriae | A fringe or fringed border |
Coliform that is adhesions, endotoxins, enterotoxins (gastroenteritis) | E Coli |
Coliform that is nonmotile, capsules, digestive and respiratory tracts | Klebsiella |
Klebsiella pneumaoniae | Destroys alveoli, bloody sputum |
Non coli forms Enterobacteria... | Cannot ferment lactose |
Non-Coliform Entero.. Proteus mirabillis | Bacilli, Gram neg., facultative anaerobe, |
Few polar flagella in liquid culture and petrichious flagella culture on agar plate culture | Proteus mirabillis |
Facultative | The power of doing or not doing something |
True pathogenic Enterobacteria...characteristics | Salmonella, Shigella, Yersenia- all type 111 secretion systems |
Salmonella how many strains | 2500 |
Salmonella produces | Hydrogen sulfide |
Characteristics of salmonella | Gram neg, motile, |
Motile | Movement |