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Module 10 Set 7
Question | Answer |
---|---|
rate increases as | [substrate] increases |
no further increase occurs after | all enzyme molecules are saturated with substrate |
Km: | substrate concentration req'd by enzyme to operate at half its maximum velocity |
Km: affinity of | enzyme to substrate |
lower Km | higher rate/ affinity |
Vmax: | rate of product formation when enzyme is saturated with the substrate operating as fast as possible |
each enzyme has specific | pH and temperature optima |
denaturation: | loss of enzyme’s structure and activity when temperature and pH rises too much above optima |
usually optimum temperature is close to | maximum temperature |
enzyme production MUST be | controlled in cells |
noncompetitive inhibitor | binds enzyme at site other than active site; changes enzyme’s shape so that it becomes less active |
competitive inhibitor | directly competes with binding of substrate to active site |
you can overcome inhibition by | adding more substrate |
Thomas Cech and Sidney Altman discovered | some RNA molecules also can catalyze reactions |
Examples of splicing ribozymes | catalyze peptide bond formation; self-splicing; and involved in self-replication |