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Unit 2
| Question | Answer |
|---|---|
| What is the principle of the E Test? | a quantitative method of antibiotic sensitivity testing that applies both dilution of antibiotic and diffusion of antibiotic into the medium. |
| E Test are usually performed on which type of organisms? | fastidious, slow-growing or nutritionally deficient that would not routinely grow in the MircoScan panel and/or generate MIC data |
| How do you read/interpret an E Test? What are the units | read where the ellipse intersects the scale where the concentration of the antibiotic inhibits growth. ug/ml |
| What is the principle of the Agar Dilution Test? | antibacterial concentration and organisms to be tested are brought together on an agar based medium. Each agar equals 1 concentration. |
| What is the Serum Bacteriocidal Test? | a direct method for measuring the antibacterial potency of a patient's serum against his own isolate of bacteria |
| What is another name for the Serum Bacteriocidal Test? | Schlicter test |
| What two serum samples are required for the Schlicter Test? What dilutions are prepared? | trough specimen and a peak specimen. Two fold serial dilutions of each specimen |
| What standardizes all E Tests? What agar is used to perform this test? | 0.5 McFarland Standard. Mueller Hinton |
| What principle do the E Test, Agar Dilution, and Schlicter Test all test? | Antimicrobial susceptibility |
| How many dilutions are plated in the Agar Dilution test? | six dilutions of one drug requiring 6 plates |
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slints
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