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Unit 2

What is the principle of the E Test? a quantitative method of antibiotic sensitivity testing that applies both dilution of antibiotic and diffusion of antibiotic into the medium.
E Test are usually performed on which type of organisms? fastidious, slow-growing or nutritionally deficient that would not routinely grow in the MircoScan panel and/or generate MIC data
How do you read/interpret an E Test? What are the units read where the ellipse intersects the scale where the concentration of the antibiotic inhibits growth. ug/ml
What is the principle of the Agar Dilution Test? antibacterial concentration and organisms to be tested are brought together on an agar based medium. Each agar equals 1 concentration.
What is the Serum Bacteriocidal Test? a direct method for measuring the antibacterial potency of a patient's serum against his own isolate of bacteria
What is another name for the Serum Bacteriocidal Test? Schlicter test
What two serum samples are required for the Schlicter Test? What dilutions are prepared? trough specimen and a peak specimen. Two fold serial dilutions of each specimen
What standardizes all E Tests? What agar is used to perform this test? 0.5 McFarland Standard. Mueller Hinton
What principle do the E Test, Agar Dilution, and Schlicter Test all test? Antimicrobial susceptibility
How many dilutions are plated in the Agar Dilution test? six dilutions of one drug requiring 6 plates
Created by: slints