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Immunoheme Lab Exam

QuestionAnswer
A A antigen, anti-B antibody
B B antigen, anti-A antibody
O no antigen, anti-A and anti-B antibody
AB A and B antigen, no antibodies
somatic cells are diploid or haploid diploid
the most common blood group antigens are of what chemical composition glycoproteins/lipids
Name the 2 immunoglobulin classes that comprise most blood group antibodies IgM and IgG
Expose you and determine your blood type histoantigens
No H antigen (hh genotype) bombay
type 1 linkage beta 1-3 linkage; secretions, H substance
Type 2 linkage beta 1-4 linkage; RBC antigens
H+H = O
H+A= A
H+B B
H+A+B= AB
what term is defined as the basic unit of inheritance gene
Genetic makeup of a trait genotype
physical expression of a trait phenotype
Why is the ABO blood group the most clinically significant acute hemolytic transfusion reactions
Universal blood donor O
Universal blood receiver AB
Universal plasma donor AB
Universal plasma recipient O
Looking for antibody? React with patient cells
Looking for antigen? React with anti-serum
Forward typing determines.... antigen
Reverse typing determines.. antibodies
What does LISS do? enhancement tool, negates negative charge on RBCs so they can stack together
Which FUT gene goes with H antigen enzyme FUT1
Which FUT gene goes with secretor enzyme FUT2
Which FUT gene goes with lewis enzyme FUT3
80% of the population has this gene secretor gene
bind better and bind complement IgM
Larger IgM
Room temperature or less (won't cause physical harm during cross over because it won't react) IgM
Direct agglutination IgM
Smaller IgG
Can cross the placenta and cause hemolytic disease of the newborn IgG
Indirect agglutination IgG
Worse for cross-over IgG
Genes make antigens in what two ways? Directly and enzymatically
what is the most common blood group in caucasian americans O+
what is the least common blood group in general AB-
what is the bombay genotype hh
why can bombay people only receive bombay blood? they have anti-h group and all other groups have H antigen
what enzyme codes the A gene; sugar transferred nacetylgalactosaminyltransferase; nacetylgalactosamine
what enzyme codes the B gene; sugar transferred galactosyltransferase; galactose
what enzyme codes the H gene; sugar transferred
what does DAT stand for? direct antibody testing
what does IAT stand for? indirect antibody testing
where is the antibody located when you're testing DAT? cells
where is the antibody located when testing IAT? plasma
What does DAT also go by? Direct coombs test
what does IAT also go by? antibody screen
antibody to lewis Le a- b- ; IgM
Additive to extend storage life of RBC's and whole blood CPDA-1
how do platelets need to be stored @ room temperature in an agitator (keeps them mixed so they don't stick together and the pH stable)
is the lewis system technically a blood group? no, antigen isn't manufactured by RBCs. Done by tissues and dumped into the plasma where RBC's adsorb it
two FUT genes = two fucose = Leb
parabombay has ABH antigens in what but not in what? secretions, RBCs
bombay phenotype has no ABH antigens in secretions or on cells
what is AHG Anti-human globulin
what genetic theory is the most recent and accepted as correct? tippett
Anti-D gives you problems with... future transfusions and pregnancies
Which genetic theory has no little d and has antithetical alleles (you get one or the other) Fisher-Race
Numbers assigned to letters and their assigned positive or negative Rosenfield terminology
What causes 1. increased osmotic fragility 2. mild hemolytic anemia 3. stomatocytes Rh null
what is the volume of a typical unit of whole blood 450 ml +- 45 ml
4 components of whole blood donation RBCs, AHF, fresh frozen plasma, platelets
what is the purpose of citrate? anticoagulant
what is the purpose of phosphate? buffers solution
what is the purpose of dextrose energy
what is the purpose of adenine increases survival (preservative)
3 important elements for platelet storage temperature (rm. temp), gentle agitation, storage duration (5 days)
what is the gene product for the se gene alpha 1,2 fucoslytransferase