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BL2410 Lab 2

Comprehensive set of policies, procedures, and practices necessary to make sure a labs results are reliable Quality assurance
Set of lab procedures designed to ensure that a test method is working properly and results meet the diagnostic needs of the physician Quality control
Pre-analytical Specimen collection and identification, specimen transport, specimen storage
Analytical Test procedures, Reagents, Instrumentation
Post-Anyalytical Technical competence of lab personnel, continuing education of lab personnel
How do you perform QC on a refractometer Check with distilled water; should read 1.000
How do you perform QC on a reagent strip Purchase control urine, prepare positive and negative controls and test once every 24 hours
How do you perform QC on a microscopic sediment exam Purchase urine with a known sediment in it
Why is CCMS the specimen of choice for urinalysis reduces the incidences of cellular and microbial contamination
What can osmometry tell you that specific gravity test cant Osmometry is a more exact measurement. It gives you a value instead of a range
Heat generate from crystallization that brings the sample to it's freezing point heat of fusion
Normal range for urine 500-850 mOsm/kg of water
First morning specimens >850 mOsm/kg of water
Random specimen 300-900 mOsm/kg of water
Normal range for serum 280-300 mOsm/kg of water
Urine to serum osmolality ratio 1.0-3.0
Explain how a freezing point osmometer works Serum is put in a cooling container, cooled below freezing point, stirred and partially crystalizes. Heat is generated (heat of fusion) and brings the sample to it's freezing point. Osmolality can be calculated from freezing point depression
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