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MICROBIOLOGY LAB
QUIZ 1 STUDY
Term | Definition |
---|---|
WHERE DO YOU PLACE YOUR PERSONAL BELONGINGS AT FOR LAB? | FRONT OF ROOM, UNDER THE WHITE BOEARD |
EATING AND DRINKING IN LAB ARE ACCEPTABLE? | NO, IT IS NOT ALLOWED. |
AT THE START OF EVERY LAB, WHAT IS THE FIRST PERSON THERE SUPPOSED TO DO? | GET A CADDY, CLEAN LAB BENCH WITH DISINFECTANT. |
WHAT TYPE OF DISINFECTANT DO WE USE? | LYSOL |
WHAT COLOR IS THE DISINFECTANT WE CLEAN WITH? | YELLOW |
WHAT SHOULD BE DONE WITH CONTAMINATED INSTRUMENTS, SUCH AS INOCULATING LOOPS AND NEEDLES THAT WE USE IN LAB? | THESE INSTRUMENT HAVE TO BE STERILIZED BY INCINERATION. |
WHAT SHOULD BE DONE WITH CONTAMINATED INSTRUMENTS, SUCH AS PIPETTES? | LARGE ONES WILL BE AUTO-CLAVED; THE SMALL ONES DISPOSED OF IN DESIGNATED RECEPTACLES. |
WHEN SHOULD YOU WASH YOUR HANDS ON A ROUTINE LAB DAY? | BEFORE AND AFTER LAB. |
WHAT SHOULD YOU RINSE YOUR HANDS WITH? | 95% ETHYL ALCOHOL. |
ARE YOU REQUIRED TO WEAR A LAB COAT? | YES |
WILL YOU BE ALLOWED TO DO THE LAB WITHOUT A LAB COAT? | NO |
WHY ARE LAB COATS IMPORTANT TO US? | THEY PROTECT OUR CLOTHING FROM CONTAMINATION AND FROM ANY DISCOLORATION BY STAINING SOLUTIONS. |
ARE YOU REQUIRED TO WEAR GLOVES THROUGHOUT LAB? | NO, HOWEVER, YOU MAY IF YOU FEEL LIKE YOU NEED TOO. THEY ARE AVAILABLE. |
WHY SHOULD YOU PULL YOUR HAIR BACK IF YOU HAVE LOOSE HAIR? | YOU DO NOT WANT IT TOUCHING ANY MATERIALS TO GET CONTAMINATED AND YOU ALSO DO NOT WANT IT TO CATCH FIRE. |
HOW DO WE CARRY, TRANSFER, AND STORE TUBE CULTURES? | USING A TEST-TUBE RACK. |
NAME THE DUAL PURPOSE OF USING TEST-TUBE RACKS IN LAB? | TO PREVENT ACCIDENTS AND TO AVOID CONTAMINATION OF SELF/ENVIRONMENT. |
WHAT ACTION IS STRICTLY PROHIBITED IN LAB?? | REMOVING ANY MEDIA, EQUIPMENT OR MICROBIAL CULTURES. |
WHAT DO YOU DO IF A CULTURE IS SPILLED OR BROKEN? | SPILLED CULTURE IS COVERED, THEN SATURATED IN LYSOL DISINFECTANT FOR 15 MINUTES. AFTER 15 MINUTES OF REACTION TIME, REMOVE THE TOWELS AND DISPOSE OF THEM IN THE BIO-HAZARD BAG AT THE BACK OF THE LAB. |
IS THE USE OF 'PIPETTE-BY-MOUTH STILL IN USE? | NO!!! THIS IS STRICTLY PROHIBITED. NEVER PUT ANY BROTH CULTURES OR CHEMICAL REAGENTS TO/IN YOUR MOUTH. |
IS IT OKAY TO LICK THE LABELS WE USE IN LAB? | NO! NEVER LICK THE LABELS! WE WILL USE SELF-STICK LABELS TO IDENTIFY DIFFERENT CULTURES. |
IS THE TERM "MEDIA" PLURAL OR SINGULAR? | PLURAL |
WHAT IS THE SINGULAR TERM FOR "MEDIA?" | MEDIUM |
WHAT IS AGAR? | IT IS A |
WHAT IS A NUTRITIVE? | IT IS A PREPARATION USED FOR GROWING MICROBES; HAS SUBSTANCE, SUCH AS SUGAR, TO HELP THE MICROBES GROW |
WHAT ARE THE 3 PHYSICAL FORMS OF MEDIA? | 1- BROTHS/LIQUIDS 2-SEMISOLID 3-SOLID |
WHAT ARE THE 3 SUBCATEGORIES OF THE PHYSICAL SOLID FORM? | 1- SLANTS 2-DEEPS 3-PLATES |
HOW MUCH WATER PER TUBE IS USED FOR BROTHS/LIQUIDS? | 6ML/TUBE |
A SEMISOILD CONTAINS WHAT PERCENTAGE OF AGAR? | 0.7% AGAR |
HOW MUCH WATER IS IN A SEMISOLID TUBE? | 10ML/TUBE |
WHAT PERCENTAGE OF AGAR DOES A SOLID CONTAIN? | 1.5% AGAR |
"SLANTS" HAVE HOW MANY ML/TUBE? | 10ML/TUBE |
"DEEPS" HAVE HOW MANY ML/TUBE? | 10ML/TUBE |
"PLATES" HAVE HOW MANY ML/TUBE? | 20ML/PLATE |
WHAT IS A CHEMICALLY DEFINE (SYNTHETIC) COMPOSITION? | IT IS MADE IN A LAB AND CONTAINS SPECIFIC CHEMICALS IN KNOWN AMOUNTS. THIS TYPE WILL HAVE A SPECIFIC LIST OF INGREDIENTS USED. |
WHAT IS COMPLEX (NON-SYNTHETIC) COMPOSITION? | IT IS ENRICHED WITH EXTRACTS SUCH AS: BEEF, YEAST, BRAIN, BLOOD, HEART, SOY OR PEPTONE. IN THE INGREDIENTS, YOU WILL SEE THE TERM "EXTRACT". THERE WILL BE A LIST OF MEASURED OUT INGREDIENTS. |
A CHEMICALLY DEFINED COMPOSITION IS ALSO CALLED? | SYNTHETIC |
A COMPLEX DEFINED COMPOSITION IS ALSO CALLED? | NON-SYNTHETIC |
WHAT IS THE FIRST STEP IN MEDIA PREPARATION? | DETERMINE THE AMOUNT OF INGREDIENTS YOU WILL NEED. |
WHAT IS THE SECOND STEP IN MEDIA PREPARATION? | MEASURE THE INGREDIENTS. |
WHAT IS THE THIRD STEP IN MEDIA PREPARATION? | MIX WITH WATER AND DISSOLVE WITH HEAT. |
WHAT IS THE FOURTH STEP IN MEDIA PREPARATION? | DISPENSE AND CAP |
WHAT IS THE FIFTH STEP IN MEDIA PREPARATION? | STERILIZE. |
IN WHAT STEP DO YOU "DO THE MATH?" | STEP ONE |
WHEN MEASURING INGREDIENTS, WHAT CONTAINER DO YOU USE FOR THE WATER? | A FLASK |
WHEN MEASURING INGREDIENTS, WHAT CONTAINER DO YOU USE FOR THE MEDIUM? | WHAT THEY CALL A "BOAT" |
WHEN MEASURING THE MEDIUM, WHAT INSTRUMENT DO YOU USE ? | A SCALE |
NOW, YOU TURN ON THE SCALE, PLACE THE 'BOAT' ON THE SCALE, WHAT YOU DO NEXT? | YOU NEED TO MAKE SURE TO 'ZERO' OUT THE SCALE AGAIN ONCE BOAT IS PLACED. |
YOU TURN ON THE SCALE, WHAT'S THE FIRST THING YOU DO NOW? | MAKE SURE IT IS SET TO "GRAMS" |
WHAT DO YOU MIX THE INGREDIENTS IN? | THE FLASK |
WHY DO WE USE CAPS? | TO PREVENT CONTAMINATION, WHILE ALLOWING OXYGEN INSIDE. |
NAME DIFFERENT CAP TYPES? | PLASTIC, METAL, SCREW TOP, COTTON, AND FOAM. |
IN THE PREPARATION STEPS OF A MEDIUM, WHY IS IT OKAY FOR STEPS 4 AND 5 TO BE INTERCHANGED? | DEPENDING ON THE EQUIPMENT USED DETERMINES THIS. FOR EXAMPLE, A PETRI DISH IS PLASTIC AND CANNOT BE USED IN THE AUTO-CLAVE, SO, AFTER THE MIXTURE IS DISSOLVED, THE FLASK IS PUT IN AND STERILIZED BEFORE BEING POURED INTO PETRI DISHES. |
HOW DO WE DISPENSE MEDIA INTO TUBES? | BY USE OF A PIPETTE. |
DISPENSE FIRST, THEN STERILIZE IS USED FOR WHAT TYPES OF MEDIA? | BROTHS, LIQUIDS, SLANTS, AND DEEPS. |
STERILE FIRST THEN DISPENSE IS USED FOR WHAT TYPES OF MEDIA? | POUR PLATES/PETRI DISHES |
ONCE A POUR PLATE HAS BEEN DISPENSED AND SOLIDIFIED, HOW SHOULD THEY BE STORED? | IN AN INVERTED POSITION. |
WHY ARE PETRI STORE IN AN INVERTED POSITION? | TO PREVENT CONTAMINATION FROM CONDENSATION AND TO PREVENT EXCESSIVE/SPREADING GROWTH. |
PETRI PLATS SHOULD _________________ BE STORED IN THE _______________ POSITION TO PREVENT CONTAMINATION AND THE EXCESSIVE/SPREADING GROWTH. | ALWAYS; INVERTED |
WHAT IS STERILIZATION? | IT IS THE REMOVAL OF ALL LIVING ORGANISMS. |
WHAT IS THE METHOD WE USE MOST TO STERILIZE IN LAB? | MOIST HEAT |
WHAT IS AN EXAMPLE OF MOIST HEAT THAT ARE COMMONLY USED IN LABS FOR STERILIZATION? | AUTOCLAVE |
WHAT ARE THE RULES FOR THE AUTOCLAVE? | 121 DEGREES CELSIUS AT 15 POUNDS OF PRESSURE FOR 15 MINUTES. |
WHAT ARE THE 6 AGAR'S WE WILL USE IN THIS LAB? | 1-NUTRIENT AGAR (NA) 2-BLOOD AGAR 3-TRYPTIC SOY AGAR (TSA) 4-MANNITOL SALT AGAR (MSA) 5-EOSIN-METHYLENE BLUE AGAR (EMB) 6-MACCONKEY AGAR (MAC) |
WHAT 2 AGARS WILL GROWN PRACTICALLY ANY MICROORGANISM? | NA AND TSA |
GIVE AN EXAMPLE OF WHAT MICROBE WOULD GROWN ON THE BLOOD AGAR? | STREPTOCOCCUS |
GIVE AN EXAMPLE OF WHAT MICROBE WOULD GROWN ON MSA (MANNITOL SALT AGAR)? | STAPHLOCOCCI |
WHY DOES THE MSA INHIBIT THE GROWTH OF MOST MICROBES? | BECAUSE IT HAS A SALT CONCENTRATION OF 7.5%. |
WHAT DOES E. COLI GROWN IN? | EMB AND MAC |
WHAT DO EMB AND MAC HAVE IN COMMON? | THEY BOTH INHIBIT THE GROWTH OF GRAM POSITIVE BACTERIA. |
THIS AGAR HAS INHIBITORY ACTION OF CRYSTAL VIOLET ON THE GROWTH OF GRAM POSITIVE ORGANISMS THAT ALLOW FOR ISOLATION OF GRAM-NEGATIVE BACTERIA. | MACCONKEY AGAR |
EOSIN-METHYLENE BLUE AGAR IS ALSO CALLED WHAT? | LEVINE |
WHAT DO YOU DO IN CASE OF A FIRE IN THE LAB? | EVACUATE THE ROOM AND ASSEMBLE OUTSIDE THE BUILDING. |
WHERE IS THE EVACUATION PROTOCOL FOUND? | IT IS POSTED AT EACH DOOR. |
WHERE DO THE PIPETTES GET DISCARDED AT IN OUT LAB? | INTO THE JAR LABELED "5%LYSOL" |
WHAT DOES THE BIOHAZARD SYMBOL MEAN? | INDICATED PROCEDURES THAT MAY POSE A HEALTH CONCERN. |
WHAT GOES INTO THE BIOHAZARD BAGS? | TEST TUBES, PETRI DISHES, SWABS, MATERIALS USED TO CLEAN UP A CULTURE SPILL. |
WHAT DO YOU DO IF YOU CATCH FIRE? | THERE IS A FIRE BLANKET, HOWEVER, USE THE 'STOP, DROP AND ROLL' METHOD. |
WHERE IS THE FIRE EXTINGUISHER LOCATED? | AT THE BACK OF THE ROOM |
WHAT DOES NOT GO INTO THE BIOHAZARD BAG? | LIQUIDS |