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MICROBIOLOGY LAB

QUIZ 1 STUDY

TermDefinition
WHERE DO YOU PLACE YOUR PERSONAL BELONGINGS AT FOR LAB? FRONT OF ROOM, UNDER THE WHITE BOEARD
EATING AND DRINKING IN LAB ARE ACCEPTABLE? NO, IT IS NOT ALLOWED.
AT THE START OF EVERY LAB, WHAT IS THE FIRST PERSON THERE SUPPOSED TO DO? GET A CADDY, CLEAN LAB BENCH WITH DISINFECTANT.
WHAT TYPE OF DISINFECTANT DO WE USE? LYSOL
WHAT COLOR IS THE DISINFECTANT WE CLEAN WITH? YELLOW
WHAT SHOULD BE DONE WITH CONTAMINATED INSTRUMENTS, SUCH AS INOCULATING LOOPS AND NEEDLES THAT WE USE IN LAB? THESE INSTRUMENT HAVE TO BE STERILIZED BY INCINERATION.
WHAT SHOULD BE DONE WITH CONTAMINATED INSTRUMENTS, SUCH AS PIPETTES? LARGE ONES WILL BE AUTO-CLAVED; THE SMALL ONES DISPOSED OF IN DESIGNATED RECEPTACLES.
WHEN SHOULD YOU WASH YOUR HANDS ON A ROUTINE LAB DAY? BEFORE AND AFTER LAB.
WHAT SHOULD YOU RINSE YOUR HANDS WITH? 95% ETHYL ALCOHOL.
ARE YOU REQUIRED TO WEAR A LAB COAT? YES
WILL YOU BE ALLOWED TO DO THE LAB WITHOUT A LAB COAT? NO
WHY ARE LAB COATS IMPORTANT TO US? THEY PROTECT OUR CLOTHING FROM CONTAMINATION AND FROM ANY DISCOLORATION BY STAINING SOLUTIONS.
ARE YOU REQUIRED TO WEAR GLOVES THROUGHOUT LAB? NO, HOWEVER, YOU MAY IF YOU FEEL LIKE YOU NEED TOO. THEY ARE AVAILABLE.
WHY SHOULD YOU PULL YOUR HAIR BACK IF YOU HAVE LOOSE HAIR? YOU DO NOT WANT IT TOUCHING ANY MATERIALS TO GET CONTAMINATED AND YOU ALSO DO NOT WANT IT TO CATCH FIRE.
HOW DO WE CARRY, TRANSFER, AND STORE TUBE CULTURES? USING A TEST-TUBE RACK.
NAME THE DUAL PURPOSE OF USING TEST-TUBE RACKS IN LAB? TO PREVENT ACCIDENTS AND TO AVOID CONTAMINATION OF SELF/ENVIRONMENT.
WHAT ACTION IS STRICTLY PROHIBITED IN LAB?? REMOVING ANY MEDIA, EQUIPMENT OR MICROBIAL CULTURES.
WHAT DO YOU DO IF A CULTURE IS SPILLED OR BROKEN? SPILLED CULTURE IS COVERED, THEN SATURATED IN LYSOL DISINFECTANT FOR 15 MINUTES. AFTER 15 MINUTES OF REACTION TIME, REMOVE THE TOWELS AND DISPOSE OF THEM IN THE BIO-HAZARD BAG AT THE BACK OF THE LAB.
IS THE USE OF 'PIPETTE-BY-MOUTH STILL IN USE? NO!!! THIS IS STRICTLY PROHIBITED. NEVER PUT ANY BROTH CULTURES OR CHEMICAL REAGENTS TO/IN YOUR MOUTH.
IS IT OKAY TO LICK THE LABELS WE USE IN LAB? NO! NEVER LICK THE LABELS! WE WILL USE SELF-STICK LABELS TO IDENTIFY DIFFERENT CULTURES.
IS THE TERM "MEDIA" PLURAL OR SINGULAR? PLURAL
WHAT IS THE SINGULAR TERM FOR "MEDIA?" MEDIUM
WHAT IS AGAR? IT IS A
WHAT IS A NUTRITIVE? IT IS A PREPARATION USED FOR GROWING MICROBES; HAS SUBSTANCE, SUCH AS SUGAR, TO HELP THE MICROBES GROW
WHAT ARE THE 3 PHYSICAL FORMS OF MEDIA? 1- BROTHS/LIQUIDS 2-SEMISOLID 3-SOLID
WHAT ARE THE 3 SUBCATEGORIES OF THE PHYSICAL SOLID FORM? 1- SLANTS 2-DEEPS 3-PLATES
HOW MUCH WATER PER TUBE IS USED FOR BROTHS/LIQUIDS? 6ML/TUBE
A SEMISOILD CONTAINS WHAT PERCENTAGE OF AGAR? 0.7% AGAR
HOW MUCH WATER IS IN A SEMISOLID TUBE? 10ML/TUBE
WHAT PERCENTAGE OF AGAR DOES A SOLID CONTAIN? 1.5% AGAR
"SLANTS" HAVE HOW MANY ML/TUBE? 10ML/TUBE
"DEEPS" HAVE HOW MANY ML/TUBE? 10ML/TUBE
"PLATES" HAVE HOW MANY ML/TUBE? 20ML/PLATE
WHAT IS A CHEMICALLY DEFINE (SYNTHETIC) COMPOSITION? IT IS MADE IN A LAB AND CONTAINS SPECIFIC CHEMICALS IN KNOWN AMOUNTS. THIS TYPE WILL HAVE A SPECIFIC LIST OF INGREDIENTS USED.
WHAT IS COMPLEX (NON-SYNTHETIC) COMPOSITION? IT IS ENRICHED WITH EXTRACTS SUCH AS: BEEF, YEAST, BRAIN, BLOOD, HEART, SOY OR PEPTONE. IN THE INGREDIENTS, YOU WILL SEE THE TERM "EXTRACT". THERE WILL BE A LIST OF MEASURED OUT INGREDIENTS.
A CHEMICALLY DEFINED COMPOSITION IS ALSO CALLED? SYNTHETIC
A COMPLEX DEFINED COMPOSITION IS ALSO CALLED? NON-SYNTHETIC
WHAT IS THE FIRST STEP IN MEDIA PREPARATION? DETERMINE THE AMOUNT OF INGREDIENTS YOU WILL NEED.
WHAT IS THE SECOND STEP IN MEDIA PREPARATION? MEASURE THE INGREDIENTS.
WHAT IS THE THIRD STEP IN MEDIA PREPARATION? MIX WITH WATER AND DISSOLVE WITH HEAT.
WHAT IS THE FOURTH STEP IN MEDIA PREPARATION? DISPENSE AND CAP
WHAT IS THE FIFTH STEP IN MEDIA PREPARATION? STERILIZE.
IN WHAT STEP DO YOU "DO THE MATH?" STEP ONE
WHEN MEASURING INGREDIENTS, WHAT CONTAINER DO YOU USE FOR THE WATER? A FLASK
WHEN MEASURING INGREDIENTS, WHAT CONTAINER DO YOU USE FOR THE MEDIUM? WHAT THEY CALL A "BOAT"
WHEN MEASURING THE MEDIUM, WHAT INSTRUMENT DO YOU USE ? A SCALE
NOW, YOU TURN ON THE SCALE, PLACE THE 'BOAT' ON THE SCALE, WHAT YOU DO NEXT? YOU NEED TO MAKE SURE TO 'ZERO' OUT THE SCALE AGAIN ONCE BOAT IS PLACED.
YOU TURN ON THE SCALE, WHAT'S THE FIRST THING YOU DO NOW? MAKE SURE IT IS SET TO "GRAMS"
WHAT DO YOU MIX THE INGREDIENTS IN? THE FLASK
WHY DO WE USE CAPS? TO PREVENT CONTAMINATION, WHILE ALLOWING OXYGEN INSIDE.
NAME DIFFERENT CAP TYPES? PLASTIC, METAL, SCREW TOP, COTTON, AND FOAM.
IN THE PREPARATION STEPS OF A MEDIUM, WHY IS IT OKAY FOR STEPS 4 AND 5 TO BE INTERCHANGED? DEPENDING ON THE EQUIPMENT USED DETERMINES THIS. FOR EXAMPLE, A PETRI DISH IS PLASTIC AND CANNOT BE USED IN THE AUTO-CLAVE, SO, AFTER THE MIXTURE IS DISSOLVED, THE FLASK IS PUT IN AND STERILIZED BEFORE BEING POURED INTO PETRI DISHES.
HOW DO WE DISPENSE MEDIA INTO TUBES? BY USE OF A PIPETTE.
DISPENSE FIRST, THEN STERILIZE IS USED FOR WHAT TYPES OF MEDIA? BROTHS, LIQUIDS, SLANTS, AND DEEPS.
STERILE FIRST THEN DISPENSE IS USED FOR WHAT TYPES OF MEDIA? POUR PLATES/PETRI DISHES
ONCE A POUR PLATE HAS BEEN DISPENSED AND SOLIDIFIED, HOW SHOULD THEY BE STORED? IN AN INVERTED POSITION.
WHY ARE PETRI STORE IN AN INVERTED POSITION? TO PREVENT CONTAMINATION FROM CONDENSATION AND TO PREVENT EXCESSIVE/SPREADING GROWTH.
PETRI PLATS SHOULD _________________ BE STORED IN THE _______________ POSITION TO PREVENT CONTAMINATION AND THE EXCESSIVE/SPREADING GROWTH. ALWAYS; INVERTED
WHAT IS STERILIZATION? IT IS THE REMOVAL OF ALL LIVING ORGANISMS.
WHAT IS THE METHOD WE USE MOST TO STERILIZE IN LAB? MOIST HEAT
WHAT IS AN EXAMPLE OF MOIST HEAT THAT ARE COMMONLY USED IN LABS FOR STERILIZATION? AUTOCLAVE
WHAT ARE THE RULES FOR THE AUTOCLAVE? 121 DEGREES CELSIUS AT 15 POUNDS OF PRESSURE FOR 15 MINUTES.
WHAT ARE THE 6 AGAR'S WE WILL USE IN THIS LAB? 1-NUTRIENT AGAR (NA) 2-BLOOD AGAR 3-TRYPTIC SOY AGAR (TSA) 4-MANNITOL SALT AGAR (MSA) 5-EOSIN-METHYLENE BLUE AGAR (EMB) 6-MACCONKEY AGAR (MAC)
WHAT 2 AGARS WILL GROWN PRACTICALLY ANY MICROORGANISM? NA AND TSA
GIVE AN EXAMPLE OF WHAT MICROBE WOULD GROWN ON THE BLOOD AGAR? STREPTOCOCCUS
GIVE AN EXAMPLE OF WHAT MICROBE WOULD GROWN ON MSA (MANNITOL SALT AGAR)? STAPHLOCOCCI
WHY DOES THE MSA INHIBIT THE GROWTH OF MOST MICROBES? BECAUSE IT HAS A SALT CONCENTRATION OF 7.5%.
WHAT DOES E. COLI GROWN IN? EMB AND MAC
WHAT DO EMB AND MAC HAVE IN COMMON? THEY BOTH INHIBIT THE GROWTH OF GRAM POSITIVE BACTERIA.
THIS AGAR HAS INHIBITORY ACTION OF CRYSTAL VIOLET ON THE GROWTH OF GRAM POSITIVE ORGANISMS THAT ALLOW FOR ISOLATION OF GRAM-NEGATIVE BACTERIA. MACCONKEY AGAR
EOSIN-METHYLENE BLUE AGAR IS ALSO CALLED WHAT? LEVINE
WHAT DO YOU DO IN CASE OF A FIRE IN THE LAB? EVACUATE THE ROOM AND ASSEMBLE OUTSIDE THE BUILDING.
WHERE IS THE EVACUATION PROTOCOL FOUND? IT IS POSTED AT EACH DOOR.
WHERE DO THE PIPETTES GET DISCARDED AT IN OUT LAB? INTO THE JAR LABELED "5%LYSOL"
WHAT DOES THE BIOHAZARD SYMBOL MEAN? INDICATED PROCEDURES THAT MAY POSE A HEALTH CONCERN.
WHAT GOES INTO THE BIOHAZARD BAGS? TEST TUBES, PETRI DISHES, SWABS, MATERIALS USED TO CLEAN UP A CULTURE SPILL.
WHAT DO YOU DO IF YOU CATCH FIRE? THERE IS A FIRE BLANKET, HOWEVER, USE THE 'STOP, DROP AND ROLL' METHOD.
WHERE IS THE FIRE EXTINGUISHER LOCATED? AT THE BACK OF THE ROOM
WHAT DOES NOT GO INTO THE BIOHAZARD BAG? LIQUIDS
Created by: salsaffar041485