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Microbiology Chapter 3

Ocular lens (eye piece) Re-magnifies the image formed by the objective lens (10x)
Body tube Transmits the image from the objective lens to the ocular lens
Objective lens Primary lenses that magnify the specimen
Stage holds the microscope slide in position
Condenser Focuses light through specimen
Diaphragm Controls the amount of light entering the condensor
Illuminator Light source
Parts of a Compound Microscope (Ocular) Ocular lens, Body Tube, Arm, Objective Lenses, Stage, Condenser, Diaphragm, Illuminator, Coarse Focusing knob, Base, Fine focusing knob
Parts of a Compound Microscope (Objective) Ocular Lens, Line of vision, path of light, prism, body tube, objective lenses, specimen, condenser lenses, illuminator, Base with source of illuminator.
Brightfield Illumination "Stain to see"
Resolving power or Resolution the ability to distinguish two adjacent points as separate and distinct
1/2 The absolute limit of resolving power is roughly ___ the wavelength of light used to illuminate the specimen.
400-800nm Visible light
.2 micrometers 200 nanometers equals
.2 um Cells in a smear have to be spread ____um apart for clarity
Darkfield Microscopy Uses an opaque disk that block the light from entering the objective causing a dark view.
Darkfield Microscopy As the light passes through the specimen it bends back into the field of view.
Darkfield Microscopy Great for ultra-thin organisms that approach the limits of resolution such as "Treponema pallidum"
Phase-Contrast Microscopy Uses a ring shaped diaphragm in the condenser and a ring-shaped diffraction plate in the objective.
Phase-Contrast Microscopy This amplifies the subtle differences in diffraction as light passes through tissues of different refractive indexes.
Phase-Contrast Microscopy Can view specimen without staining.
10x Ocular magnification
100x Oil immersion (objective) lens
40x Hi-dry (objective) lens
10x (objective) Low (objective) lens
4x Scan (objective) lens
Fluorescence, Fluorescence Microscopy takes advantage of ______, the ability of substances to absorb short wavelengths of light (ultraviolet) and give off light at a longer wavelength (visible).
Fluorescent Dye, (Fluorescence Microscopy) Bacterium is stained with ____.
Illuminate, (Fluorescence Microscopy) UV light is used to ____.
Shorter, longer Dye absorbs the ____ wavelength (UV) and emits a ____ fluorescent color.
antigens (foreign substances) used as a diagnostic tool by finding specific ____ with a fluorescent antibody tag
Electron Microscopy A beam of electrons is used instead of light
Electron Microscopy Electrons produce a much shorter wavelength than visible light giving a much better resolution so that increased magnification allows viewing of smaller items
Transmission Electron Microscopy (TEM) a finely focused beam of electrons from an electron gun passes through a specifically prepared, ultra-thin section of the specimen.
Transmission Electron Microscopy (TEM) Uses thin slices of the specimen
Transmission Electron Microscopy (TEM) Shoots electrons in a vacuum
Transmission Electron Microscopy (TEM) Electrons pass through specimen and expose photographic paper
Transmission Electron Microscopy (TEM) Resolution .0025 um
10,000x-100,000x Transmission Electron Microscopy (TEM) magnification
Scanning Electron Microscope (SEM) Beam of electrons directed and bounced off the hard Au dusted surface of the specimen
Scanning Electron Microscope (SEM) Electron collected to make a 3-D image of the surface
.02um Scanning Electron Microscope (SEM) Resolution
1,000x-10,000x Scanning Electron Microscope (SEM) Magnification
Created by: jtzuetrong



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