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BACT250-02
Microbiology Chapter 3
| Term | Definition |
|---|---|
| Ocular lens (eye piece) | Re-magnifies the image formed by the objective lens (10x) |
| Body tube | Transmits the image from the objective lens to the ocular lens |
| Objective lens | Primary lenses that magnify the specimen |
| Stage | holds the microscope slide in position |
| Condenser | Focuses light through specimen |
| Diaphragm | Controls the amount of light entering the condensor |
| Illuminator | Light source |
| Parts of a Compound Microscope (Ocular) | Ocular lens, Body Tube, Arm, Objective Lenses, Stage, Condenser, Diaphragm, Illuminator, Coarse Focusing knob, Base, Fine focusing knob |
| Parts of a Compound Microscope (Objective) | Ocular Lens, Line of vision, path of light, prism, body tube, objective lenses, specimen, condenser lenses, illuminator, Base with source of illuminator. |
| Brightfield Illumination | "Stain to see" |
| Resolving power or Resolution | the ability to distinguish two adjacent points as separate and distinct |
| 1/2 | The absolute limit of resolving power is roughly ___ the wavelength of light used to illuminate the specimen. |
| 400-800nm | Visible light |
| .2 micrometers | 200 nanometers equals |
| .2 um | Cells in a smear have to be spread ____um apart for clarity |
| Darkfield Microscopy | Uses an opaque disk that block the light from entering the objective causing a dark view. |
| Darkfield Microscopy | As the light passes through the specimen it bends back into the field of view. |
| Darkfield Microscopy | Great for ultra-thin organisms that approach the limits of resolution such as "Treponema pallidum" |
| Phase-Contrast Microscopy | Uses a ring shaped diaphragm in the condenser and a ring-shaped diffraction plate in the objective. |
| Phase-Contrast Microscopy | This amplifies the subtle differences in diffraction as light passes through tissues of different refractive indexes. |
| Phase-Contrast Microscopy | Can view specimen without staining. |
| 10x | Ocular magnification |
| 100x | Oil immersion (objective) lens |
| 40x | Hi-dry (objective) lens |
| 10x (objective) | Low (objective) lens |
| 4x | Scan (objective) lens |
| Fluorescence, Fluorescence Microscopy | takes advantage of ______, the ability of substances to absorb short wavelengths of light (ultraviolet) and give off light at a longer wavelength (visible). |
| Fluorescent Dye, (Fluorescence Microscopy) | Bacterium is stained with ____. |
| Illuminate, (Fluorescence Microscopy) | UV light is used to ____. |
| Shorter, longer | Dye absorbs the ____ wavelength (UV) and emits a ____ fluorescent color. |
| antigens (foreign substances) | used as a diagnostic tool by finding specific ____ with a fluorescent antibody tag |
| Electron Microscopy | A beam of electrons is used instead of light |
| Electron Microscopy | Electrons produce a much shorter wavelength than visible light giving a much better resolution so that increased magnification allows viewing of smaller items |
| Transmission Electron Microscopy (TEM) | a finely focused beam of electrons from an electron gun passes through a specifically prepared, ultra-thin section of the specimen. |
| Transmission Electron Microscopy (TEM) | Uses thin slices of the specimen |
| Transmission Electron Microscopy (TEM) | Shoots electrons in a vacuum |
| Transmission Electron Microscopy (TEM) | Electrons pass through specimen and expose photographic paper |
| Transmission Electron Microscopy (TEM) | Resolution .0025 um |
| 10,000x-100,000x | Transmission Electron Microscopy (TEM) magnification |
| Scanning Electron Microscope (SEM) | Beam of electrons directed and bounced off the hard Au dusted surface of the specimen |
| Scanning Electron Microscope (SEM) | Electron collected to make a 3-D image of the surface |
| .02um | Scanning Electron Microscope (SEM) Resolution |
| 1,000x-10,000x | Scanning Electron Microscope (SEM) Magnification |
Created by:
jtzuetrong
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