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Gram positive test
Gram-positive lab algorithm
Question | Answer |
---|---|
S. aureus | Catalase +, Coagulase +, clusters of coci |
Coagulase -, novobiocin sensitive | S. epidermis |
Coagulase -, novobiocin resistant | S. saprophyticus (2 S's because its "strong") |
Partial hemolysis is known as ___ and looks like ____ on agar media | Partial hemolysis (green) ---> alpha (use this for streptococci |
Complete hemolysis is known as ___ and looks like ___ on an agar media | complete hemolysis (clear) ---> beta |
No hemolysis is known as ___ and looks like ___ on an agar media | no hemolysis ---> gamma |
Hemolysis is used to find types of ___ after performing ___ | Use hemolysis to distinguish between types of streptococci |
When do you use novobiocin? what is novobiocin resistant? sensitive? | Use to determine between species of staph. Staph epidermidis is coagulase negative, Novobiocin sensitive, Staph saprophyticus is coagulase, novobiocin resistant. |
Catalse is used to determine between ____, coagulase is used to determine between ____. | Staph (+) and Strep (-). Coagulase = S. Aureus (+), S. saprophyticus, epidermidis (-) |
Optochin is used to determine between ___ and ____. Optochin sensitive is bile ____ wherease Optochin resistant is bile ___. These species belong in the ____ hemolytic group. | Optochin = determine difference between S. Pneumoniae (sensitive) and Viridans streptococci (resistant). alpha. |
When is bacitracin used? ____ is bacitracin sensitive, while ____ is bacitracin resistant. These belong to what species? | Bacitracin sensitive = Group A (S. pyogenes), Bacitracin resistant = Group B (S. agalactiae). These belong to the betal hemolytic group. |
what groups belong to the gamma group. | Enterococcus (E. faecalis) and Peptostreptococcus (anaerobe) |
Clostridium (Name tests) | Gram + rod |
Listeria | Gram + rod |
S. Aureus | Gram + cocci, Catalase + in clusters, coagulase positive |
Streptococcus | Gram + cocci, catalase - |
S. pneumoniae | Gram + cocci, catalse - , green, optochin sensitve, bile soluble |
S. pyogenes | Gram + cocci, catalase -, clear, bacitracin sensitive |
S. epidermidis | Gram + cocci, catalase +, coagulase -, novobiocin sensitive |
S. Saprophyticus | Gram + cocci, catalase +, coagulase -, novobiocin resistant |
Novobiocin resistant ___, Novobiocin sensitive ____ | Novobiocin resistant = Saprophyticus, novobiocin sensitive = epidermidis |
Optochin resistant _____, Optochin sensitive | Optochin resistant= S. viridans (s. mutans), optochin sensitive = S. Pneumoniae |
Bacitracin resistant ____, Bacitracin sensitive _____. | Bacitracin Resistant= group B strep (S. agalactiae), Bacitracin sensitive = group A strep (S. pyogenes) |