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Gram positive test

Gram-positive lab algorithm

QuestionAnswer
S. aureus Catalase +, Coagulase +, clusters of coci
Coagulase -, novobiocin sensitive S. epidermis
Coagulase -, novobiocin resistant S. saprophyticus (2 S's because its "strong")
Partial hemolysis is known as ___ and looks like ____ on agar media Partial hemolysis (green) ---> alpha (use this for streptococci
Complete hemolysis is known as ___ and looks like ___ on an agar media complete hemolysis (clear) ---> beta
No hemolysis is known as ___ and looks like ___ on an agar media no hemolysis ---> gamma
Hemolysis is used to find types of ___ after performing ___ Use hemolysis to distinguish between types of streptococci
When do you use novobiocin? what is novobiocin resistant? sensitive? Use to determine between species of staph. Staph epidermidis is coagulase negative, Novobiocin sensitive, Staph saprophyticus is coagulase, novobiocin resistant.
Catalse is used to determine between ____, coagulase is used to determine between ____. Staph (+) and Strep (-). Coagulase = S. Aureus (+), S. saprophyticus, epidermidis (-)
Optochin is used to determine between ___ and ____. Optochin sensitive is bile ____ wherease Optochin resistant is bile ___. These species belong in the ____ hemolytic group. Optochin = determine difference between S. Pneumoniae (sensitive) and Viridans streptococci (resistant). alpha.
When is bacitracin used? ____ is bacitracin sensitive, while ____ is bacitracin resistant. These belong to what species? Bacitracin sensitive = Group A (S. pyogenes), Bacitracin resistant = Group B (S. agalactiae). These belong to the betal hemolytic group.
what groups belong to the gamma group. Enterococcus (E. faecalis) and Peptostreptococcus (anaerobe)
Clostridium (Name tests) Gram + rod
Listeria Gram + rod
S. Aureus Gram + cocci, Catalase + in clusters, coagulase positive
Streptococcus Gram + cocci, catalase -
S. pneumoniae Gram + cocci, catalse - , green, optochin sensitve, bile soluble
S. pyogenes Gram + cocci, catalase -, clear, bacitracin sensitive
S. epidermidis Gram + cocci, catalase +, coagulase -, novobiocin sensitive
S. Saprophyticus Gram + cocci, catalase +, coagulase -, novobiocin resistant
Novobiocin resistant ___, Novobiocin sensitive ____ Novobiocin resistant = Saprophyticus, novobiocin sensitive = epidermidis
Optochin resistant _____, Optochin sensitive Optochin resistant= S. viridans (s. mutans), optochin sensitive = S. Pneumoniae
Bacitracin resistant ____, Bacitracin sensitive _____. Bacitracin Resistant= group B strep (S. agalactiae), Bacitracin sensitive = group A strep (S. pyogenes)
Created by: ddecampo