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Stack #22140

Baier--LE Topic 9

TermVocabulary
ruler (metric) a tool used to measure the length of an object. Common units are um=micrometers(microns), mm=millimeters, cm=centimeters & m=meters
micrometers (microns) a small unit (1/1000 of a mm) used to measure the length of an object using a microscope.
graduated cylinder a tool often used to measure a liquid's volume. Remember to take your measurement from the bottom of the meniscus.
volume amount of space an object occupies.
temperature "usually" measured in degrees Celsius. Remember 0 degrees C = freezing, 37 degrees C = body temperature and 100 degrees C = boiling
mass amount of matter in an object. Kilograms and grams are common units.
balance (triple-beam or electronic) a tool used to measure the mass of an object.
microscope a tool that uses a lens or lenses to enlarge the image of an object.
magnification how much the microscope enlarges the image of an object. To calculate MULTIPLY the eyepiece(ocular) TIMES the current object being used.
stereo or dissecting microscope a tool that has 2 lenses (hence stereo) and uses low magnification (10-20X's) to increase the viewability of a specimen you are dissecting.
compound microscope a tool that has one lens and we will commonly use to view the largest organelles within cells.
microscope image (only compound) the enlarged image will be upside- down and backwards.
eyepiece or ocular lens or lenses that you view image through. In compound microscope is usually 10X's.
Objective lenses lens closest to the object, usually attached to revolving nosepiece and contains 4X scanning, 10X low power an 40X high power.
diaphragm located under stage and contols the amount of light or intensity.
course adjustment knob a large knob used only on low power to coarsely focus.
fine adjustment knob a small knob used on any power to sharpen the focus and allows the viewer to focus on different levels.
gel electrophoresis a technique used to SEPERATE either DNA or proteins BY SIZE.
chromatography a technique used to SEPERATE a mixture of molecules. We used this process to SEPERATE leaf pigments.
indicators a substance that changes color when it comes in contact with a specific substance.
Iodine (Lugol's) solution an indicator for starch turns from amber to dark blue-black AND a stain. We used this stain to view onion cells.
Benedict's Blue Solution an indicator that turns orangish to brick red when in the presence of glucose. Must heat this one to see a reaction.
Stain is used to enhance (stain) the organelles within cells. The trade-off for increased viewability is the stain kills the cells.
methylene blue a stain we used to view our cheek cells
Dichotomous Key a key used to classify organisms based on characteristics.
Created by: Webster
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