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ch16 Transcription
ch16
| Question | Answer |
|---|---|
| Transcription occurs when | RNA pol matches the base in a ribonucl. triphosphate with the complem. base in a gene |
| How does the initiation begins? | RNA pol and sigma form a holoenzyme which contains the active site for catalysis |
| What the name of the sites where the holoenzyme binds to? | promoters |
| Promoters are | sections of DNA wehre the trascription begins |
| Sigma is responsible for | guiding RNA pol to specific locations where transcription should begin |
| The promoter for bacteria is a segment of DNA located on a non-template strand similar to Tataat and known as | -10 box |
| Sigma opens what to start the transcription? | the helix |
| The -10 box is centered 10 bases upstream from what? | the transcription start site |
| The place where the transcription begins is called | +1 site |
| -35 bases upstream are called what | -35 box |
| Then the transcription begins when sigma does what? | binds to the -35 and -10 boxes |
| Sigma is what | regulatory protein |
| Sigma tells RNA pol in bacteria where and when | to start synthesizing Rna |
| Sigma is released once | Rna synthesis is under way and initiation is complete |
| During elongation phase, RNA pol moves along DNA template in the | 3 to 5 direction of the template strand, synthesizing RNA in the 5 to 3 direction |
| Transcription ends with a | termination phase where formation of a hairpin in the mRNA disrupts the transcription |
| The bases that make up the termination signal code for a stretch of RNA with an unusual property: | as soon as its synthesized, the RNA sequence folds back on itself and forms a short double helix |