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Microbiology Exam 1
Microbiology- UALR- Dr. Ali
Question | Answer |
---|---|
The structure of DNA was discovered by __________ | James Watson and Francis Crick (1953) |
Probably the most studied microbe: | Bacteria |
Joseph Lister was credited with the implementation of ________ technique. | Aseptic technique (19th century) |
Collectively microbes constitute roughly _____% of the earth's biomass. | 60% |
One of the most significant benefits that microbes provide is the ability to synthesize _____ | DNA |
Who developed the compound microscope? | British Scientist Robert Hooke (17th century) |
The "father of modern microbiology" | Louis Pasteur |
Because microbes reproduce relatively quickly they are particularly useful in ________ studies. | Genetic |
Some of Louis Pasteur's contributions: | Swan-necked flask, pasturization, the fermention process |
Earth is the planet of _______ | Bacteria |
Microbiology is: | The study of microbes. The study of organisms so small you need a microscope to study them. |
A type of virus containing a nucleic acid and lacking a protein covering. | Viroid |
The theory that states that microorganisms can arise from non-living material. | Spontaneous Generation Theory |
2 dimensions of the scope of Microbiology: | The study of the kinds of microbes and the kind of work that microbiologists do. |
The vast majority of microorganisms are directly or indirectly __________. | Beneficial |
Matthias Schleiden and Theodor Schwann formulated what? | The Cell Theory |
The supposed first microbiology course was taught by ________________ ______________ | Robert Koch |
Robert Koch invented the technique to prepare _____________ _______________ | Pure cultures |
Robert Koch worked as a _________________ for most of his casreer. | Physician |
Who developed the simple microscope? | Anton Von Leeuwenhoek (17th-18th century) |
The tiniest of all microbes: | Virus |
Compaired to other organisms, microbes have relatively _________________ structures. | Simple |
Less than _____% of known microbes cause disease. | 1% |
___________ ____________ is credited with seeing the first living organism "animalecules." | Anton Von Leeuwenhoek |
The theory that states that microorganisms can invade other organisms and cause disease. | The Germ Theory of Disease |
Bacteria do not have a __________ | Nucleus |
Pasteur aided greatly in the development of ______________ | Vaccines |
The "swan-necked" flask is associated with ______________ _______________ | Louis Pasteur |
Most microbes are _____cellular | uni- |
Francesco Redi devised what experiment to disprove spontaneous generation? | Meat in jars |
What theory states that cells are the fundamental unit of life | Cell theory |
A virus that has protein and is lacking nucleic acid: | Prion |
The smallest chemical unit of matter | Atom |
2 or more atoms chemically combined: | Molecule |
The most common elements in all forms of life: | Carbon, Hydrogen, Oxygen, Nitrogen |
Atomic Number | The number of protons in an atom |
Atomic Weight | Total number of protons and neutrons in an atom |
Ion | An atom or molecule that had gained or lost one or more electrons. |
Acid | A hydrogen ion donor (accepts H+ ions in solution) |
Base | A proton acceptor; hydroxyl ion donor (releases OH- ions in solution) |
Anion | An ion that had gained an electron, becoming negatively charged. |
Isotope | Atoms of the same element that contain different numbers of neutrons. |
The key element that all life is made up of: | Carbon |
Particles too large to form true solutions | Colloids |
Matter that is composed of one kind of atom | Element |
Protons and Neutrons are located where? | Nucleus |
The mass of a proton or neutron is almost exactly equal to _____ | 1 AMU |
Atoms of molecules are held together by _________ bonds. | Chemical |
Ionic Bond | Formed by the attraction of oppositely charged ions |
Covalent Bond | When atoms share pairs of electrons |
Mole | Same as "gram molecular weight," the weight of a substance in grams equal to the sum of the atomic weights of the atoms in a molecule of the substance. |
Radioisotopes | Unstable isotopes that emmit subatomic particles and radiation. |
Atoms of molecules are held together by what kind of bonds? | Chemical |
Catabolism | The breaking down of molecules (the release of energy) |
Anabolism | The synthesis of larger molecules (uses energy) |
Water is a __________ compound and has a high ___________ __________ | Polar; surface tension |
A pH of "7" is __________ | Neutral |
A pH above "7" is ________ | Basic |
A pH below "7" is ________ | Acidic |
The study of Carbon-containing compounds | Organic Chemistry |
LIpids are insoluable in ________ | Water |
Lipids are soluable in _________ | Non-polar solvents |
Fats consist of ______ and _______ ________ | Glycerol and fatty acids |
Phospholipids contain a phosphate group instead of a _________ __________ | Fatty acid |
Steroids have a complex ______-ring structure | 4 |
Proteins consist of ______ ________ linked by ________ bonds | amino acids; peptide |
Enzymes increase the rate of _______ _______ in living organisms | Chemical Reactions |
The simplest of carbohydrates | Monosaccharides |
The body's primary use for carbohydrates is ________ | Energy |
Monosaccharides can form together to form ___________ & __________ | Disaccharides; polysaccharides |
Nucleotides consist of what three parts? | Nitrogenous base, a five-carbon sugar, and one or more phosphate groups |
Nulceic Acids consist of lon polymers of nucleotides called ____________________ | Polynucleotides |
The 2 nucleic acids that are found in living organisms | Ribonucleic Acid (RNA) and Deoxyridonucleic Acid (DNA) |
Enzymes are protein _________ | Catalysts |
Viruses have either ______ or ____ but not both. | DNA or RNA |
Functional Group | The part of a molecule that generally participates in chemical reactions as a unit and that gives the molecule some of its chemical properties. |
Oxidation | The addition of oxygen or the removal of H+ ions or electrons in a substance |
Reduction | The removal of oxygen or the addition of H+ ions or electrons in a substance. |
Monosaccharides consist of: | A carbon chain or ring with several alcohol groups and one other functional group. |
Several monosaccharides are _________ and they have the same __________ ____________ | Isomers; molecular formula (but different structures and properties) |
The most abundant monosaccharide | Glucose |
Monosaccharides can be reduced to become ___________ & _________ | Doxy sugars and sugar alcohols |
Diasaccharides are formed when 2 monosaccharides are connected by the removal of __________ (dehydration synthesis) _________ bond. | Water; glycosidic |
Glycosidic Bond | A sugar alcohol/sugar linkage |
Polysacchairdes are formed when many ___________ are linked together. | Monosaccharides |
Polymer | A long chain of repeating units |
Triacylglycerol | A fat formed when 3 fatty acids are bonded to glycerol. |
The smaller the distance between objects distinguished, the __________ the resolving power. | Greater |
A numerical measure ofthe resolution that can be obtained with a specific lens | Resolving Power |
The measurement from crest to crest or trough to trough | Wavelength |
Resolving power is dependent on the ________ source | Light |
Constant value for a particular lens | Numerical Aperture |
The ability of a microscope to differentiate between two distinct structures | Resolution |
Micrometer | 10^-6 |
Nanometer | 10^-9 |
Immersion oil is used to prevent loss of ______ resulting from refraction. | Light |
Total magnification is ________ lens magnifying power, multiplied by ocular lens magnifying power. | Objective |
____________ light is used in Florescence Microscopy | Ultraviolet |
Electron Microscopy uses a beam of _________ instead of a beam of light. | Electrons |
Transmission Electron Microscopy is used to see the _________ structures of microbes. | Internal |
Scanning Electron Microscopy is used to see the __________ structures of microbes. | External/ Surface |
Cationic dyes are _______ | Basic/positive |
Anionic dyes are _______ | Acidic/negative |
Negatively charged bacterial cell walls bind with _________ dye. | Cationic |
Prokaryotic cells do not have a defined _____________ | Nucleus |
Eukaryotic cells have a _____ nucleus containing _______ | True; DNA |
Cell walls provide a characteristic ________; they also prevent the cell from ______ | Shape; bursting |
3 Bacterial cell wall types: | Gram positive; Gram negative; Acid-fast |
Instead of a true nucleus, bacteria have a _________ ________ | Nuclear Region |
The bacteria's nuclear region is composed primarily of: | DNA (some RNA and protein) |
Ribosomes consist of __________ and __________ | RNA and protein |
Flagellum are made up of what protein? | Flagellin |
Bacteria with one single flagellum | Monotirchous |
Bacteria with one flagellum at each end | Amphetrichous |
Bacteria with two or more flagella at each end | Lophotrichous |
Bacteria that have flagella all over | Peritrichous |
Bacteria with no flagella | Atrichous |
Chemotaxis | The movement of bacteria in response to a chemical |
Phototaxis | The movement of bacteria in response to light |
Positive Chemotaxis | Movement of a bacterium toward a chemical |
Negative Chemotaxis | Movement of a bacterium away from a chemical |
Pili help bacterium _________ to nutrition sources | Attach |
The type of pili that is associated with bacterial reproduction | Conjugation Pili |
Eukaryotic cells are more/less complex than prokaryotic cells. | More |
Psudopodia is the movement of ____________ | Cytoplasm |
Cell membranes are ____________. (meaning they only allow select material in and out) | Semipermeable |
Type of cell that have a nucleus and membrane-enclosed structure: | Eukaryotic Cell |
Carbohydrates are bonded by: | Glycosidic Bonds |
Lipids are bonded by: | Ester Bonds |
Amino Acids are bonded by: | Peptide Bonds |
Nucleic Acid/ Nulceotides are bonded by: | Phosphodiester Linkage |
Type of cell that lacks nucleus and other membrane-enclosed structures: | Prokaryotic Cell |
Where is DNA located in eukaryotic cells? | In membrane enclosed nucleus |
Where is DNA located in prokaryotic cells? | In nuclear region |
List the 3 domains: | Archaea Bacteria Eukarya |
Viruses are _____________ | Acellualr |
Bacteria have a (large or small) surface to volume ratio? | Large |
Spherical bacteria | Coccus |
Rod-like bacteria | Bacillus |
"comma- shaped" bacteria | Vibrio |
"wavy-shaped" bacteria | Spirillum |
"corkscrew-shaped" bacteria | Spirochete |
Structurally, bacterium consists of what? | 1. Cell membrane 2. Cytoplasm 3. Exteranl Structures (capsules, flagella, and pili) |
What nucleotides are found in DNA? | Adenine, thymine, guanine, and cytosine (binding: AT=weaker bond and GC=stronger bond) |
What nucleotides are found in RNA? | Adenine, uracil, guanine, and cytosine (binding: AU and GC) |
The _________ _________ lies outside the cell membrane. | Cell wall |
2 Important functions of cel wall: | 1. Maintains cell shape 2. prevents cell from burstin when fluid enters in |
The single most important component of the bacterial cell wall: | Peptidoglycan |
Additional molecule found in walls of Gram-Positive bacteria: | Teichoic Acid |
Teichoic Acid in the Gram-Positive bacterial cell wall probably serves as a passageway for movement of _____ into and out of cells. | Ions |
Outermembranes are primarily found in what kind of bacteria? | Gram-negative |
Lipopolysacchride (LPS) is also called: | Endotoxin |
Part of the outer layer that can be used to identify Gram-Negative Bacteria: | LPS |
Lipopolysacchride consists of: | Ploysacchrides and Lipid A |
NADH produces how many ATP? | 3 ATP |
FADH produces how many ATP? | 2 ATP |
GTP produces how many ATP? | 1 ATP |