Question 1

Bacteriology? Phycology? Mycology? Protozoology? Parasitology? Virology? Microbial Metabolism?

Accepted Answer

1. study of bacteria/Archaea.
2. study of Algae.
3. study of Fungi.
3. study of Protozoan.
4. study of parasitic protozoa and parasitic animals.
5. Biochemistry: chemical reactions in cells.

Question 2

Microbial Genetics? Environmental Microbiology? Serology? Immunology? Epidemiology?

Accepted Answer

1. study of RNA/DNA.
2. study of microbes/organisms in their environment.
3. study of antibodies in serum.
4. study of the body's defense against specific diseases.
5. study of frequency, distribution and spread of diseases.

Question 3

Etiology? Infection Control? Chemotherapy? Bioremediation? Public Health Remediation?

Accepted Answer

1. study of the cause of diseases.
2. promoting hygiene and controlling nosocomial infections.
3. drugs to treat an infection.
4. use of microbes to remove pollutants.
5. sewer, water purification and control of insects that spread diseases.

Question 4

Agricultural Microbiology? Food and Beverage Technology? Pharmaceutical Microbiology? Recombinant DNA Technology?

Accepted Answer

1. use of microbes to control pest.
2. reduce/eliminate microbes from food/drinks.
3. create vaccines and antibiotics.
4. alternate microbial genes to synthesize useful products.

Question 5

Prokaryotes? Types?(2)
Eukaryotes? Types?(4)
Both cells?

Accepted Answer

Prokaryotes: no nucleus or organelles.
Types: bacteria and archaea.
Eukaryotes: have nucleus and organelles.
Types: Protozoa, Arthropods, Helminths, Fungi and Algae.
Both: have cell membranes cytoplasm and genetic DNA.

Question 6

Acellular Infectious Agents Types?(3)
Non-photosynthetic Types?
Photosynthetic Types?(1)

Accepted Answer

Acellular Types: Viruses, Viroid and Piron.
Non-photosynthetic Type: Fungi
Photosynthetic: Cyanobacteria.

Question 7

Unicellular Cells?
Multicellular Cells?
Acellular Agents?

Accepted Answer

Unicellular: Bacteria, Archaea, Algae, Fungi(yeast), Protozoa
Multicellular: Algae, Fungi(mold), Helminths

Question 8

Macroorganisms? Types?
Microorganisms? Types?
Infectious Agents Types?

Accepted Answer

Macro: organisms large enough to not be seen with microscope.
Types: Arthropods, Helminths
Micro: organisms needed to be seen with scope.
Types: Bacteria, Archaea, Fungi, Algae, and Protozoa
Infectious: Virus, Pirons and Viroids

Question 9

Dark Field Microscope? Bright Field Microscope?

Accepted Answer

1. see living, colorless(white), unstained organisms. dark background/bright specimen.
2. see killed, stained specimens and counting the microorganism.

Question 10

Fluorescence Microscope? Confocal Microscope?

Accepted Answer

1. localize specific structures and used for diagnosis tool. dark background/bright fluorescent specimen.
2. shows details of structures with different dyes. structures are stained with specific structures.

Question 11

Phase Contrast Microscope? Differential Interference Contrast Microscope?

Accepted Answer

1. observe internal structures. the specimen has light and dark areas.
2.observe internal structures. image is 3-D.

Question 12

Transmission Electron Microscope? Scanning Electron Microscope?

Accepted Answer

1. detail of internal structures in bacteria and viruses. 2-D image. 
2. surface detail. 3-D image.

Question 13

Scanning Tunneling Probe Microscope? Atomic Force Probe Microscope?

Accepted Answer

1.observe surface of objects at atomic and molecular level.
2. observe living specimens at molecular and atomic level.

Question 14

Simple Stain?

Accepted Answer

single dye use, to observe the size, morphology and arrangement of the specimen. results in a purple or blue color.

Question 15

Gram Stain?

Accepted Answer

uses 2 dye to determining the difference between 2 large groups of microorganism. Resulting in pink for negative and purple for positive.

Question 16

Acid Fast Stain? Endospores Stain?

Accepted Answer

1. highlights Mycobacteria and Nocardia. results in pink-red for acid fast or blue for non-acid fast. 
2.shows endospores form the Bacillus and Clostridium Genera. results in green for endospores and pink-red for vegetation.

Question 17

Negative Stain for Capsules? Flagellar Stain?

Accepted Answer

1. shows bacterial capsules. results in black background with white specimen.
2. shows the number of flagellum. results in visible flagellum.

Question 18

Taxonomy? Classification? Strains?

Accepted Answer

1. identify, classify and name organisms.
2. organize large diversity of organisms.
3. parts of species.

Question 19

Hierarchy of Taxa? Duhh, Keep Pot Clean Or Family Gets Sick!

Accepted Answer

Domain(based on rRNA 1.eukaryote 2.bacteria 3.archaea), Kingdom, Phylum, Class, Order, Family, Genus, Species.

Question 20

Scientific Names? Formula?

Accepted Answer

1.refer to the same species.
2. Take Genus (capitalized), Use Specific Epithet(lowercase) and italicize(Online Test *hint) if written underline.

Question 21

Heat Fixation? Chemical Fixation? Resolution? Refraction?

Accepted Answer

1. passing a slide smear up on a Bunsen burner.
2. applying  a chemical like methyl to the smear.
3. distinguishing objects that are close together.
4. bending of magnification.

Exam One

Microbiology Lecture

Term	Definition
Bacteriology? Phycology? Mycology? Protozoology? Parasitology? Virology? Microbial Metabolism?	1. study of bacteria/Archaea. 2. study of Algae. 3. study of Fungi. 3. study of Protozoan. 4. study of parasitic protozoa and parasitic animals. 5. Biochemistry: chemical reactions in cells.
Microbial Genetics? Environmental Microbiology? Serology? Immunology? Epidemiology?	1. study of RNA/DNA. 2. study of microbes/organisms in their environment. 3. study of antibodies in serum. 4. study of the body's defense against specific diseases. 5. study of frequency, distribution and spread of diseases.
Etiology? Infection Control? Chemotherapy? Bioremediation? Public Health Remediation?	1. study of the cause of diseases. 2. promoting hygiene and controlling nosocomial infections. 3. drugs to treat an infection. 4. use of microbes to remove pollutants. 5. sewer, water purification and control of insects that spread diseases.
Agricultural Microbiology? Food and Beverage Technology? Pharmaceutical Microbiology? Recombinant DNA Technology?	1. use of microbes to control pest. 2. reduce/eliminate microbes from food/drinks. 3. create vaccines and antibiotics. 4. alternate microbial genes to synthesize useful products.
Prokaryotes? Types?(2) Eukaryotes? Types?(4) Both cells?	Prokaryotes: no nucleus or organelles. Types: bacteria and archaea. Eukaryotes: have nucleus and organelles. Types: Protozoa, Arthropods, Helminths, Fungi and Algae. Both: have cell membranes cytoplasm and genetic DNA.
Acellular Infectious Agents Types?(3) Non-photosynthetic Types? Photosynthetic Types?(1)	Acellular Types: Viruses, Viroid and Piron. Non-photosynthetic Type: Fungi Photosynthetic: Cyanobacteria.
Unicellular Cells? Multicellular Cells? Acellular Agents?	Unicellular: Bacteria, Archaea, Algae, Fungi(yeast), Protozoa Multicellular: Algae, Fungi(mold), Helminths
Macroorganisms? Types? Microorganisms? Types? Infectious Agents Types?	Macro: organisms large enough to not be seen with microscope. Types: Arthropods, Helminths Micro: organisms needed to be seen with scope. Types: Bacteria, Archaea, Fungi, Algae, and Protozoa Infectious: Virus, Pirons and Viroids
Dark Field Microscope? Bright Field Microscope?	1. see living, colorless(white), unstained organisms. dark background/bright specimen. 2. see killed, stained specimens and counting the microorganism.
Fluorescence Microscope? Confocal Microscope?	1. localize specific structures and used for diagnosis tool. dark background/bright fluorescent specimen. 2. shows details of structures with different dyes. structures are stained with specific structures.
Phase Contrast Microscope? Differential Interference Contrast Microscope?	1. observe internal structures. the specimen has light and dark areas. 2.observe internal structures. image is 3-D.
Transmission Electron Microscope? Scanning Electron Microscope?	1. detail of internal structures in bacteria and viruses. 2-D image. 2. surface detail. 3-D image.
Scanning Tunneling Probe Microscope? Atomic Force Probe Microscope?	1.observe surface of objects at atomic and molecular level. 2. observe living specimens at molecular and atomic level.
Simple Stain?	single dye use, to observe the size, morphology and arrangement of the specimen. results in a purple or blue color.
Gram Stain?	uses 2 dye to determining the difference between 2 large groups of microorganism. Resulting in pink for negative and purple for positive.
Acid Fast Stain? Endospores Stain?	1. highlights Mycobacteria and Nocardia. results in pink-red for acid fast or blue for non-acid fast. 2.shows endospores form the Bacillus and Clostridium Genera. results in green for endospores and pink-red for vegetation.
Negative Stain for Capsules? Flagellar Stain?	1. shows bacterial capsules. results in black background with white specimen. 2. shows the number of flagellum. results in visible flagellum.
Taxonomy? Classification? Strains?	1. identify, classify and name organisms. 2. organize large diversity of organisms. 3. parts of species.
Hierarchy of Taxa? Duhh, Keep Pot Clean Or Family Gets Sick!	Domain(based on rRNA 1.eukaryote 2.bacteria 3.archaea), Kingdom, Phylum, Class, Order, Family, Genus, Species.
Scientific Names? Formula?	1.refer to the same species. 2. Take Genus (capitalized), Use Specific Epithet(lowercase) and italicize(Online Test *hint) if written underline.
Heat Fixation? Chemical Fixation? Resolution? Refraction?	1. passing a slide smear up on a Bunsen burner. 2. applying a chemical like methyl to the smear. 3. distinguishing objects that are close together. 4. bending of magnification.

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