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Genetics Pereira

Lab Final

QuestionAnswer
SDS Sodium dodecyl sulfate breaks the cell membrane and cell wall
What is the charge of the spooling rod? Positive
How do you wrap DNA? 45 degreees clockwise
What is the handedness of DNA? right handed
What does cold etOH do? Precipitate nucleic acids
What is a plasmid Circular piece of DNA inside bacteria
White colonies= mutated
Black colonies= normal/non mutatesd
TMTC= Too many to count
SMF (spontaneous mutation frequency) formula # of Beta Gal. mutants (no UV exposure)/ Total # of viable cells (before exposure)
MF (mutation frequency) formula # of Beta Gal mutations (after UV)/ Original viable cell count
Endonucleases are also called what? Restriction Enzymes
When do endonucleases cut? When it finds a DNA sequence (recognition sequence)
In linear DNA 4 cuts makes how many pieces
Created by: acegirl5