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Genetics Pereira
Lab Final
Question | Answer |
---|---|
SDS | Sodium dodecyl sulfate breaks the cell membrane and cell wall |
What is the charge of the spooling rod? | Positive |
How do you wrap DNA? | 45 degreees clockwise |
What is the handedness of DNA? | right handed |
What does cold etOH do? | Precipitate nucleic acids |
What is a plasmid | Circular piece of DNA inside bacteria |
White colonies= | mutated |
Black colonies= | normal/non mutatesd |
TMTC= | Too many to count |
SMF (spontaneous mutation frequency) formula | # of Beta Gal. mutants (no UV exposure)/ Total # of viable cells (before exposure) |
MF (mutation frequency) formula | # of Beta Gal mutations (after UV)/ Original viable cell count |
Endonucleases are also called what? | Restriction Enzymes |
When do endonucleases cut? | When it finds a DNA sequence (recognition sequence) |
In linear DNA 4 cuts makes how many pieces |