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Microbiology
BIO 205- Chapter 13 Viruses, Viroids and Prions
| Question | Answer |
|---|---|
| Place the following in the most likely order for biosynthesis: 1. Phage lysozyme 2. mRNA 3. DNA 4. viral proteins 5. DNA polymerase | 2,5,3,4,1 |
| The molecule serving as mRNA can be incorporated in the newly synthesized virus capsids of all the following except: | -Strand RNA rhabdoviruses |
| A virus with RNA-dependent RNA polymerase: | Synthesizes double-stranded RNA from an RNA template. |
| Which of the following would be the first step in the biosynthesis of a virus with reverse transcriptase? | A complementary strand of DNA must be synthesized from an RNA template |
| An example of lysogeny in animals could be: | Latent viral infections |
| The ability of a virus to infect an organism is regulated by: | the host species, the type of cells, the availability of an attachment site, and cell factors necessary for viral replication |
| Place the following in the order in which they are found in a host cell: 1. capsid proteins 2. infective phage particles 3. phage nucleic acid | 3, 1, 2 |
| Which of the following does not initiate DNA synthesis? | A single stranded RNA virus (Togaviridae) |
| A viral species is not defined on the basis of the disease symptoms it causes. The best example of this is: | Hepatitis |
| In which of the following ways do viruses differ from bacteria? | Viruses are not composed of cells. |
| Which of the following provides the most significant support for the idea that viruses are nonliving chemicals? | They cannot reproduce themselves outside of a host. |
| Which of the following is not true about spikes? | They are used for penetration. |
| Which of the following is not used as a criterion to classify viruses? | Biochemical tests |
| Which of the following is not a method of culturing viruses? | In culture media |
| A clear area against a confluent "lawn" of bacteria is called a | Plaque |
| An infectious protein is a | Prion |
| An envelope is acquired during which of the following steps? | Release |
| Host Range | refers to the spectrum of host cells in which a virus can multiply |
| Host Range is determined by: | the specific attachment site on the host cell's surface and the availbility of host cellular factors |
| Viral size is ascertained by: | Electron microscopy |
| What is "viruses range" in length? | 20 to 1000 nm |
| A complete, fully developed viral particle composed of nucleic acid surrounded by a coat | Virion |
| T/F Viruses contain either DNA or RNA, never both | True. |
| In relation to protein in viruses ranges from about 1% to about 50%. | The porportion of nucleic acid. |
| The protein coat surrounding the nucleic acid of a virus is called: | capsid |
| Some envelopes are covered with carbohydrate-protein complexes called | Spikes |
| resemble long rods, and their capsids are hollow cylinders surrounding the nucleic acid | Helical viruses - for example Ebola virus |
| What virus is manysided? Usually the capsid is an icosahedron | Polyhedral virus - for example adenovirus |
| Enveloped helical virus | influenza virus |
| Enveloped polyhedral virus | Simplexvirus |
| Classificatioin of viruses are based on: | type of nucleic acid, strategy for replication, and morphology |
| Virus family names end in | -viridae |
| Virus genus names end in | -virus |
| A group of viruses sharing the same genetic information and ecological niche | viral species |
| _____ must be grown in living cells. | Viruses |
| What is the easiest virus to grow? | bacteriophages |
| The plaque method mixes: | bacteriophages with host bacteria and nutrient agar. |
| Each plaque originates with a single viral particle. teh concentration of viruses is given as: | plaque-forming units |
| What two viruses provide models to study human AIDS? | Simian AIDS and feline AIDS |
| Cells growing in culture media in the lab are: | Cell cultures. |
| What test are used most often to identify viruses?. | Serological tests |
| Viruses may be indentified by: | RFLPs and PCR |