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BZ 310 Exam 2
| Question | Answer |
|---|---|
| v-type pumps | uses pyrophosphate (PPi-> 2Pi) |
| pH 3 is | 1 mmol concentration of H+ |
| ion channel proteins | favored by membrane potential |
| proton antiport proteins into vacuole | Na, Ca, sucrose (H+ out to make it favorable) |
| tight junction | prevents flow between cells in the intestinal tract |
| how is glucose taken up? | 2 Na+ for every 1 glucose then ATPase removes the Na+ (also osmosis is occurring with water) |
| 3 main types of neurons | sensory, interneurons, motor |
| why are action potentials uni-directional? | refractory period of Na+ gated channels on other side that just opened |
| action potentials are a ___ ____ ____ | positive feedback loop |
| what returns membrane potential back to normal? | K+ voltage gated (out) and leak channels |
| voltage gated K+ channels are also called | shaker channels b/c of fruit flies that shake due to deficiency of them |
| where does depolarization occur? | Nodes of Ranvier, not in places where myelin is present |
| series of events at neuromuscular junction | action potential, Ca2+ channel opens, synaptic vesicle fuses w/ membrane, neurotransmitter release, acetylcholine binds, Na+ in, Ca2+ released into cytosol, muscle contraction, P-class pump moves Ca2+ back into SR |
| axon hillock | depolarization must reach potential for an AP to go; all or none principle |
| how do local anesthetics work? | block Na+ channels in sensory neurons |
| where does the majority of ATP in neurons go? | Na/K pump (3 Na out 2 K in) |
| patch clamping | salty solution over a single channel, then an electrode inside; measure current and potential |
| ΔG total= | ΔGconc + ΔGvolt |
| ATP hydrolysis pkg/kcal per mol | about 8 pkg; 10-12 kcal/mol |
| ΔGconc at 300K | -1.4 x log C0/Ci (i=intended not initial) |
| ΔGvolt= | zFV (charge x 23 x volt) |
| which transporter transports glucose from enterocyte? | glut-2 |
| pmf= | V + 60 mV x ΔpH |
| ion motive force= | V + 60 mV x log (C0/Ci) |
| simplified Nernst equation | V=60 mV x log (C0/Ci) |
| Valinomycin | allow K+ to transfer across |
| Nigericin | K+/H+ antiport ionophore |
| A23187 | Ca2+ ionophore |
| CCCP | H+ ionophore |
| NH4+ | H+ ionophore |
| Gramicidin | nonspecific pore ionophore |
| TPP+ | lipophilic cation, delocalized charges |
| anabolic pathways are (4) | endergonic, divergent, reductive, NADPH intermediate |
| catabolic pathways are (4) | exergonic, convergent, oxidative, NADH intermediate |
| reduced have __ ___ electrons | high energy |
| oxidized have ___ ____ electrons | low energy |
| negative E0 | strong electron donor |
| positive E0 | strong electron acceptor |
| what is required to break down very large FAs? | peroxisomes |
| 2 regulated enzymes in glycolysis | hexokinase and phosphofructokinase |
| pyruvate -> acetyl coA what enzyme? | pyruvate dehydrogenase |
| reactions in mitochondria | pyruvate -> acetate + CO2 + NADH |
| guanine vs adenine | guanine has a double bond oxygen |
| FAD/FMN | flavins |
| max ATP made from one glucose | would be 38 but 2 used up to get into mitochondria |
| palmityl coA -> | 31 NADH + 15 FADH2 + 8 ATP = 131 ATP |
| lactate -> pyruvate cost | 6 ATP in liver (back to glucose) |
| 0-3 secounds | ATP stocks |
| 2-10 seconds | creatine-P |
| 7-60 seconds | anaerobic metabolism + respiration |
| 1min- 90 min | respiration (glucose and fats) |
| over 90 minutes | fats only |
| pmf is used for ___ in bacteria | motility |
| oxidation is ___ to pmf generation | coupled |
| type 1 redox carriers | capacity for protons and elctrons (ubquinone) |
| type 2 redox carriers | capacity for only electrons (heme) |
| ferric | 3+ |
| ferrous | 2+ |
| why does the ETC use each complex rather than just jumping down? | specific binding |
| complex 4 and 1 are | conformational pumps |
| ubiquinone and complex III are | redox loops |
| why isn't 3 H+ enough per ATP? | 8-9 actual; UCP, cotransporters use energy |
| Cellular work ___ the ____ ratio | lowers the ATP/ADP ratio (metabolism is coupled) |
| Best sustained oxygen consumption (mitochondria experiment) | ADP, Pi, pyruvate, hexokinase, glucose |
| Phloem | sugar export |
| Xylem | H2O transport |
| ___% of O2 production occurs in the oceans | 50% |
| light reactions | occur in thylakoid membrane, NADPH and ATP formed |
| calvin cycle | occurs in stroma; CO2 fixed and reduced to sugars (NADPH and ATP consumed) |
| The ion in chlorophyll is | magnesium |
| Chlorophyll protein | LHC (I and II) |
| How many electrons per oxygen produced? | 4 |
| How many electrons can PQ pick up? | 2 |
| Photosystem I | light energy removes electron from P700 (ferredoxin), reduce NADP+, replenish electrons from plastocynanin |
| Strongest oxidant in nature | PS II |
| Strongest reductant in nature | P700 (ferredoxin) |
| Small trick of photosynthesis | reduce NADP with electrons from water + build pmf |
| Big trick of photosynthesis | split water without causing damage |
| Cyclic electron flow | no NADPH made, just ATP synthesis (bacteria do this) |
| Pmf chloroplasts | pH |
| Pmf mitochondria | voltage |
| Complex I/II are like | PS II |
| Ubiquinone is like | plastoquinone |
| Cyt B/C complex is like | cytochrome B/F complex |
| Cytochrome C is like | plastocyanin |
| CytC oxidase is like | PS I |
| Uncouplers + 2 examples | decreased ATP synthesis, increased electron transport, ammonia and dinitrophenol |
| Blockers + example | decreased ATP synthase and decreased electron transport, cyanide |
| Most abundant enzyme on the planet | RuBisCO |
| Per GAP produced, use | 3 CO2, 9ATP, 6NADPH |
| 2 major fates of GAP | conversion to glucose/starch in chloroplast; export for sucrose synthesis |
| why do chloroplastic genes exist? | debunk protein to protect PS II |
| how to prevent light damage (5) | light regulation, cyclic electron flow, feedback inhibition of PS II, repair of PS II, photorespiration |
| photorespiration | rubisco can react w/ O2 to form 2-P-glycolate (toxic) then convert to triose (costly) |
| photorespiration 3 organelles | chloroplast, peroxisome, mitochondria |
| special c4 anatomy | mesophyll don’t have RuBisCO, bundle sheet cells have RuBisCO but limited PSII (corn is like this) |
| glycolysis oxidation step | 6 |
| glycolysis substrate-level phosphorylation steps | 7 and 10 |
| complex III aka (2) | coenzyme Q, cytochrome C reductase |
| complex IV aka | cytochrome oxidase |
| How many net ATP are produced anaerobically per glucose? | 2 |
| How many net ATP are produced with the complete breakdown of one glucose under aerobic conditions in a eukaryote? | 36 |
| how many NADPH used per carbon atom? | 2 |
| how many ATP used per carbon atom? | 3 |