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BZ 310- Lab Quiz 2

what % agarose? .7-2%
what dye to visualize DNA? ethidium bromide (intercalating agent)
DNA migrates toward.... + electrode (smaller further)
DNA size marker DNA fragments of known size for comparison
turnover number # of product molecules formed in a minute
michaelis menten equation V= Vmax[S] / (Km + [S])
lineweaver burk plot 1/V= Km/Vmax x 1/[S] + 1/Vmax
enzyme used in kinetics lab catalase
catalase reaction 2H2O2 -> 2H2O+O2
what was the instrument called that collected the oxygen? eudiometer
what were all the different things varied? enzyme concentration, temperature, pH, substrate, inhibitor
what were the different enzyme concentrations used? 25%, 33%, 50%, 75%
what were the different pHs used? 5.8, 6.6, 7.2, 7.8, 8.8
what were the different temperatures used? 5, 20, 25, 30, 40, 60
what was the optimal enzyme concentration? 75%
what was the optimal pH? 7.2 (or 7.8)
what was the optimal temperature? 20C
whats the original substrate concentration? 8.8 M
competitive inhibition raises Km
noncompetitive inhibition lowers Vmax
what were the three primers? MAT forward primer, a reverse primer, alpha reverse primer
what was in the master mix? dNTPs, primers, buffer, Taq polymerase
what are the 3 PCR cycle phases? 15 seconds at 96 (denaturing), 30 seconds at 55 (annealing), 2 minutes at 72 (extension)
negative control yeast the empty well
positive control yeast diploid
BLAST basic local alignment search tool
PCR fragment DNA is ___ linear (so you can assume it's based on size)
mating type of unknown strain alpha
how do you find velocity from evolved O2? tangent line (initial slope)
cyanide is a ____ inhibitor noncompetitive
shrinking due to hypertonic colution crenate
bursting due to hypotonic solution hemolysis
partition coefficient comparing solubility in water versus lipid (higher= less water soluble)
L-B y intercept 1/Vmax
L-B x intercept -1/Km
what was the inhibitor? NaCN
Created by: melaniebeale