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BZ 310- Lab Quiz 2
| Question | Answer |
|---|---|
| what % agarose? | .7-2% |
| what dye to visualize DNA? | ethidium bromide (intercalating agent) |
| DNA migrates toward.... | + electrode (smaller further) |
| DNA size marker | DNA fragments of known size for comparison |
| turnover number | # of product molecules formed in a minute |
| michaelis menten equation | V= Vmax[S] / (Km + [S]) |
| lineweaver burk plot | 1/V= Km/Vmax x 1/[S] + 1/Vmax |
| enzyme used in kinetics lab | catalase |
| catalase reaction | 2H2O2 -> 2H2O+O2 |
| what was the instrument called that collected the oxygen? | eudiometer |
| what were all the different things varied? | enzyme concentration, temperature, pH, substrate, inhibitor |
| what were the different enzyme concentrations used? | 25%, 33%, 50%, 75% |
| what were the different pHs used? | 5.8, 6.6, 7.2, 7.8, 8.8 |
| what were the different temperatures used? | 5, 20, 25, 30, 40, 60 |
| what was the optimal enzyme concentration? | 75% |
| what was the optimal pH? | 7.2 (or 7.8) |
| what was the optimal temperature? | 20C |
| whats the original substrate concentration? | 8.8 M |
| competitive inhibition | raises Km |
| noncompetitive inhibition | lowers Vmax |
| what were the three primers? | MAT forward primer, a reverse primer, alpha reverse primer |
| what was in the master mix? | dNTPs, primers, buffer, Taq polymerase |
| what are the 3 PCR cycle phases? | 15 seconds at 96 (denaturing), 30 seconds at 55 (annealing), 2 minutes at 72 (extension) |
| negative control yeast | the empty well |
| positive control yeast | diploid |
| BLAST | basic local alignment search tool |
| PCR fragment DNA is ___ | linear (so you can assume it's based on size) |
| mating type of unknown strain | alpha |
| how do you find velocity from evolved O2? | tangent line (initial slope) |
| cyanide is a ____ inhibitor | noncompetitive |
| shrinking due to hypertonic colution | crenate |
| bursting due to hypotonic solution | hemolysis |
| partition coefficient | comparing solubility in water versus lipid (higher= less water soluble) |
| L-B y intercept | 1/Vmax |
| L-B x intercept | -1/Km |
| what was the inhibitor? | NaCN |
Created by:
melaniebeale
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