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special stains
Nerve Stains
Term | Definition |
---|---|
Stains for Glial Fibers | Holzer Method (preferred) Mallory Phosphotungstic Acid Hematoxylin Stain |
stains for neurofibrillary tangles and senile plaques | Thioflavin S Bielschowsky-Pas |
Stains for Myelin Sheath | Weil Method Luxol Fast Blue Luxol Fast Blue-Cresyl Echt Violet Luxol Fast Blue- Holmes Silver Nitrate Luxol Fast Blue-PAS-Hematoxylin |
Stains for Nerve Fibers | Bodian and Holmes |
Stains for Nissl Substances | Cresyl Echt Violet I Cresyl Echt Violet II (acidic) Thionin |
Thioflavin S | stains for neurofibrillary tangles and senile plaques fluorescent dye helps detect amyloid |
Thioflavin S | Alzheimer neurofibrillary tangles, senile plaques, neutrophil threads, senile plaque amyloid, cerebrovascular amyloid.... BRIGHT GREEN diffuse plaques and extracellular tangles....PALER YELLOW GREEN PSP tangles and pick bodies...NOT WELL DEMONSTRATED |
Thioflavin S | .25 potassium permanganate potassium metabisulfate sodium hydroxide-> peroxide->removes lipid autofluorescence acetic acid-> water->50% alcohol .0125% thioflavin S (agitate) |
Bielschowsky-PAS | Stain for nerve fibers, neurofibrillary tangles, and senile plaques stain for diagnosing alzheimers |
Bielschowsky-PAS | neurofibrillary tangles, peripheral neuritis of neuritic plaques....DARK BLACK axons......BLACK amyloid (plaque cores and vascular)..... Magenta Lipofuscin..... Magenta |
Bielschowsky-PAS | silver nitrate in dark ammoniacal silver ammonia water add to ammoniacal silver developer ( formaldehyde, nitric acid, citric acid) and place slides in solution ammonia water ->gold chloride-> ammonia water -> sodium thiosulfate periodic acid schiff |
Cresyl Echt Violet Method II | stain for Nissl sbstances has acidic pH restricts the staining to DNA and RNA containing structures |
Cresyl Echt Violet Method II | cresyl echt violet for 8 minutes dehydrate and mount |
Cresyl Echt Violet Method I | Stain for Nissl substances identification of neurons in tissue used to show loss of nissl substances (chromatolysis) |
Cresyl Echt Violet Method I | cresyl echt violet absolute alcohol xylene--> balsam xylene differentiate in absolute alcohol check microscopically repeat until background is clear and nissl substances are well demonstrated |
Nissl Substances | trigoid substance/ chromidal substance basophilic material in cytoplasm of neuron large aggregates of rough endoplasmic reticulum stained sharply with basophilic dyes |
Cajal Stain | for astrocytes |
Weil Method | for Myelin Sheath Myelin Sheath...BLUE TO BLACK background........LIGHT TAN |
Weil Method | staining solution (alcoholic hematoxylin, ferric ammonium sulfate) differentiate in 4% ferric ammonium sulfate until white and gray matter can be distinguished complete differentiation in sodium borate (control microscopically) ammonia water |
Luxol Fast Blue Method | for Myelin Sheath Myelin Sheath... BLUE TO BLUE-GREEN background........COLORLESS |
Luxol Fast Blue Method | hydrate to 95% alcohol luxal fast blue overnight diff in lithium carbonate cont. diff. in 70% alchol until can distinguish gray and white matter finish diff in lithium carbonate until greenish-blue of white matter contrasts with colorless gray matter |
Luxol Fast Blue- Cresyl Echt Violet | stains myelin sheath and nissl substances myelin...........................BLUE nissl substances........VIOLET nuclei........................VIOLET |
Luxol Fast Blue- Cresyl Echt Violet | overnight luxol fast blue diff in lithium carbonate then alcohol until gray and white matter can be distinguished, then litium carbonate until sharp contrast cresyl echt violet--> add acetic acid |
Luxol Fast Blue- Holmes Silver Nitrate | stain for Myelin Sheath and Nerve Fibers Myelin Sheath....BLUE TO GREEN axons and nerve fibers.....BLACK |
Luxol Fast Blue- Holmes Silver Nitrate | silver nitrate in dark impregnate overnight (boric acid, borate, pyridine, silver nitrate) reduce (hydroquinine and sodium sulfite) gold chloride, develop in oxalic acid sodium thio luxol fast blue overnight -> lithium carbonate-> diff in 70 alcohol |
Luxol Fast Blue-PAS Hematoxylin | stains myeline sheath, basement membrane, senile plaques, fungi, corpora amylacea, nuclei |
Luxol Fast Blue-PAS Hematoxylin | myelin sheath.....BLUE TO BLUE GREEN basement membrane, senile plaques, fungi, and corpora amylacea.......ROSE nuclei.......PURPLE |
Holzer Method | preferred method for glial fibers glial fibers....BLUE background.....COLORLESS |
Holzer Method for glial fibers | phosphomolybdic acid-alcohol, absolute alcohol chloroform (tissue will be translucent), crystal violet, potassium bromide--> airdry, differentiate (aniline oil, chloroform, ammonium hydroxide) |
Mallory Phosphotungstic acid hematoxylin stain | stain for glial fibers ratio phosphotungstic acid to hematein 20:1 glial fibers.... BLUE nuclei............BLUE neurons........SALMON myelin...........BLUE |
Mallory Phosphotungstic acid hematoxylin stain | mordant zenkers with acetic acid overnight, lugol iodine, decolorize in 95 alcohol, potassium permanganate, decolorize oxalic acid, stain with PTAH overnight |
Holmes Silver Nitrate | stains for Nerve Fibers and Neurofibrils argyrophilic -requires chemical reduction axons and nerve fibers.....BLACK Neurofibrils.......................BLACK |
Holmes Silver Nitrate | silver nitrate impregnate (boric acid, borax, distilled water, silver nitrate, pyridine) reduce (hydroquinone & sodium sulfate) gold chloride develop oxalic acid sodium thiosulfate, optional counterstain |
Bodian Metod | Stains for nerve fibers, nerve endings, and neurofibrils nerve fibers...........BLACK Nuclei....................BLACK Background...........LIGHT BLUE |
Wallerian degeneration | bodian method stains for this axon distal to injury disappears with myelin sheath |