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Microbiology 200
Cerritos College - Microbiology 200 - Midterm Lab Practicum
| Question | Answer |
|---|---|
| Acid-fast cell walls contain a high percentage of what? | Mycolic Acid |
| Making up 250 ml of nutrient agar below: flask + ______ g nutrient agar + ______ ml water. | 5 g and 250 ml of water |
| K/A reaction in the TSI tube indicate the fermentation of which sugar(s)? | Glucose |
| A/A reaction in the TSI tube indicate the fermentation of which sugar(s)? | Lactose, sucrose or both. |
| Enterbactericiae all ferment what? | Glucose |
| API 20E A red color in the URE (urea) tube indicates what? | Positive test result |
| API 20E A brown color in the TDA tube after the addition of ________ ___________ indicates ____________. | ferric chloride indicates positive result |
| API 20E What's the end product in a positive TDA result? | Indolepyruvic Acid |
| API 20E A colorless ONPG indicates what? | No production of beta-galactosidease. |
| API 20E ONPG mimics what? | Lactose |
| Nitrate reduction is checked in the ________ tube. Name the 3 reagents that may be required. | GLUcose tube. 1) NIT A (sulfanilic acid) 2) NIT B (Alphanaphthol) 3) Zinc (Zn) |
| Name a disease caused by an anaerobic spore-forming bacillus. | Tetanus, pseudomembranous colitis, botulism, etc. |
| What specific stain can be used to determine spore location? | Dorner Method and Shaeffer-Fulton staining method (both are endospore stains) |
| Name the anaerobic spore-forming bacillus genus. | Clostridium |
| Why is a selective media used? | To select a colony while inhibiting the growth of other unwanted colonies. |
| API 20E ONPGs substrate | O-nitophenyl-B-D-galactopyranoside |
| API 20E ADHs substrate | Arginine |
| API 20E LDCs substrate | Lysine |
| API 20E ODCs substrate | Ornithine |
| API 20E CITs substrate | Sodium citrate |
| API 20E H2Ss substrate | Sodium thiosulphate |
| API 20E UREs substrate | Urea |
| API 20E TDAs substrate | Typtophan |
| API 20E INDs substrate | Tryptophane |
| API 20E VPs substrate | creatine sodium PYRUVATE |
| API 20E GELs substrate | Kohn's charcoal gelatin |
| API 20E GLU (nitrate reduction)s substrate | potassium nitrate |
| What color will gram positive (+) organisms be? | Purple |
| What color will gram negative (-) organisms be? | pink/ red |
| True or False Oil's ____________ _________ is about the same as glass, therefore oil is used to capture the light. Prevents light from scattering. | refractive index |
| Agar is derived from what? | Derived from seaweed - dried and turned into powder. Most microbes cannot break down the carbohydrates from the agar. Agar is not food; it simply supplies surface. |
| Autoclave at ___psi -->> _____C -->> ___ minutes. | 15PSI, 121C for 15 minutes. |
| What is a culture? | Growth of live microorganisms |
| What is a culture medium? | A substance containing nutrients that supports the growth of microbes. |
| Resolution | Allows you to see with clarity. |
| RESOLVING Power's FORMULA | 0.61 (wavelength) RP= ----------------- NA Objective |
| Heat fixing allows three (3) things to occur. | 1. Kills organism 2. breaks cell wall to easily stain the organism 3. Adhere to glass |
| Enterobacteriaceae "enterica" are all: | *Gram negative (-) bacillus *non spore formers *facultative anaerobes (w or w.o oxygen) *ferment glucose *reduce nitrate (NO3) *Cytochrome oxidase negative (-) |
| API 20E In the IND tube, what reagent is used? | Covak's reagent |
| Acid clots vs. Litmus milk curd? | acid clot sticks on one side of the tube while milk curd moves away from the test tube. |
| TDA vs. IND? | TDA -->> triptophane deaminase IND -->> Tryptophenase |
| Why is TSI a combination medium? | 3 different sugars (glucose, sucrose and lactose) and breakdown of hydrogen sulfide gas. |
Created by:
jonum
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