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LD BIO REVIEW PT 4
LD BIO REVIEW PT 4 - EXPERIMENTATION
| Term | Definition |
|---|---|
| INDEPENDENT VARIABLE | the one thing that "I change" to test my hypothesis; Graph on the X Axis |
| DEPENDANT VARIABLE | the thing that changes because of the Data collected; Graph on the Y Axis |
| CONTROL GROUP | Group that is studied under the normal conditions; reference; setup in which no factor was changed |
| EXPERIMENTAL GROUP | the group that is identical to the control group with one change or difference |
| LABELING GRAPH | Title the graph, Label X & Y Axis, Determine a proper scale for X & Y Axis; & Set up a key or legend if necessary |
| EXPERIMENT MORE VALID | Repeat the experiment, Increase the number of specimens or trials & peer review |
| EYEPIECE | Part that you look through (i.e.: usually 10X) |
| OBJECTIVE LENS | Magnifying part closest to the slide (high power = usually 40X; low power = usually 10X) |
| FINE ADJUSTMENT KNOB | used to focus on low & high power |
| COURSE ADJUSTMENT KNOB | used to focus only on low power |
| STAGE | where the slide is placed |
| STAGE CLIPS | holds the slide in place |
| DIAPHRAGM | controls the amount of light used |
| TOTAL MAGNIFICATION ON LOW POWER | 10 X 10 = 100X |
| TOTAL MAGNIFICATION ON HIGH POWER | 10 X 40 = 400X |
| IMAGE OF LETTER THROUGH MICROSCOPE | Mirror image & flipped upside down; increasing magnification reduces the field (see less of the image); increasing the magnification reduces the amount of light. |
| WET MOUNT SLIDE | Put the cells on the center of a slide; put a drop of water with a dropper onto the cells; lower a cover slip slowly at an angle |
| STAIN ON SLIDE | Put a drop of the stain onto the edge of the cover slip; place a paper towel on the edge of the other side of the cover slip, the paper towel will pull the water from under the cover slip, and in turn pull the stain onto the cells. |
| PAPER CHROMATOGRAPHY | A laboratory technique that is used to separate different molecules from one another. Separation of compounds in a solution by size and colors. |
| GEL ELECTROPHORESIS | Separation of DNA using electrical current by size |
| INDICATORS FOR PH, GLUCOSE, & STARCH | Color or state change indicating the presence of a particular substance |
| DICHOTOMOUS KEYS | Tools to help in the classification of organisms |
| PROBLEM | Question for which the scientist is trying to find an answer |
| HYPOTHESIS | Is a prediction about the possible answer to the problem. Based on research, which would include any previous knowledge about the problem, and observations and information gained from studying the problem. |
| SCIENTIFIC METHOD | Problem, Hypothesis, Experiment, Observation, Conclusion & Repeat Experiment |
| CONCLUSION | Answer to the problem. |
| IODINE | Lugol's solution; Starch turns black |
| BENEDICT'S SOLUTION | Simple sugars; turns from blue to yellow |
| BROMTHYMOL BLUE | Carbon dioxide; turns from blue to yellow |
| pH PAPER | Acids & bases |
| STAINS | Used to make organelles visible |
| CELSIUS TEMPERATURE | 0 - Freezing; 100 - Boiling; 37 - Body temp; 20 - Room temp |
| SALT WATER | Added to cells cause water to leave the cell and cells shrink |
| DISTILLED WATER | Causes them to take in water and swell |