click below
click below
Normal Size Small Size show me how
Chapter 13 GENE
Genetics Chapter 13 Human Heredity
Question | Answer |
---|---|
Clones | Genetically identical molecules, cells, or organisms all derived from single ancestor |
Cloning | The production of identical copies of molecule, cells, or organisms from a single ancestor |
Charles Steward | Grew individual carrot cells in the laboratory by using special nutrients |
Callus | Ball of undifferentiated cells |
Embryo splitting | After in vitro fertilization, early embryonic cells are divided and grown into clones |
Nuclear transfer | Cell fusion, enucleated eggs are fused with embryonic adult cells and grow into clones. |
First clone horsish | Mule, Horse is mother, Donkey is father |
Problems with cloning | Viability, lifespan, genetic regulation, epigenetic regulation. |
Molecular cloning | Technology was developed to clone segments of DNA molecules, based of restriction endonucleases, that recognize and cut DNA at specific nucleotide sequences |
Recombinant DNA Technology | DNA that is created by combining two or more sequences that would not normally occur together |
Things needed to clone DNA | A way to cut DNA at specific sites, a carrier molecule to hold DNA for cloning, a place where the DNA can be copied |
Restriction enzymes | Bacterial enzymes that cut DNA at specific sites |
Vectors | Self- replicating DNA molecules used to transfer foreign DNA segments between host cells (plasmids or engineered viral chromosomes) |
Plasmids | used as vectors for cloning DNA |
DNA ligase | Joins recombinant DNA molecules |
Genomis library | A collection of clones that contain all the genetic information in an individual |
Yeast artificial chromosome | Cloning vector with telomeres and a centromere, carries DNA fragments up to 1 million bases long, uses eukaryote yeast as a host cell |
Probe | A labeled nucleic acid used to identify a complementary region in a clone of genome |
PCR | A method for amplifying DNA segments using cycles of denaturation, annealing to primers, and DNA polymerase- directed synthesis |
Mullis | Won Nobel Prize in 1993 for PCR, laser pointer story, LSD |
Southern blotting |