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Micro 2

Chapter 7

QuestionAnswer
Must be provided to an organism Essential Nutrients
required in large quantities. Play principle role in cell structure and metabolism (proteins, carbs) Macronutrients
– required in small amounts. Involved in enzyme function and maintenance of protein structure (manganese, zinc, nickel) Micronutrients/Trace Elements
Organic Nutrients – Carbon Source, usually the products of living things 1. Proteins 2. Lipids 3. Carbs 4. Methane (CH4)
– Atom or molecule that contains a combination of atoms other than carbon and hydrogen. *CO2 Inorganic Nutrients
Inorganic Nutrients 02 (Lipid synthesis an metabolism) Nitrogen (proteins, ATP, Nucleic Acids) H2O – Metabolically active Metals – Enzyme function Salts – Maintain proper osmotic pressure
CLASSIFICATIONS BY CARBON SOURCE Heterotroph Autotroph
CLASSIFICATION BY ENERGY SOURCE Chemotroph Phototroph
Chemoautotrophs Survive totally on inorganic substances
Photoautotrophs – Oxygenic Photosynthesis, Anoxygenic Photosynthesis
Methanogens A kind of chemoautotroph that produces methane gas under anaerobic conditions
Parasites Derive nutrients from host (Pathogens, Some are obligate parasites)
Transport Movement of chemicals across the cell membrane
Passive Transport does not require energy; substances exist in a gradient and move from areas of higher concentration to areas of lower concentration (Diffusion, Osmosis [Diffusion of water], Facilitated diffusion [requires a carrier]
Diffusion Movement of a molecule down its concentration gradient
Osmosis Diffusion of water across a semipermeable membrane
High solute, low solvent (water) Hypertonic
Low solute, High Solvent (water) Hypotonic
Isotonic about equal parts water on both sides
Facilitate diffusion Molecules diffuse down the concentration gradient through a transport protein embedded in plasma membrane
Active transport requires energy and carrier proteins; gradient independent; transport is against the concentration gradient
Endocytosis Eating and drinking by a cell
Phagocytosis ingests substances or cells
Pinocytosis ingests liquids
Environmental factors that influence microbes Temperature Oxygen requirements Effects of PH Osmotic pressure
Psychrophiles 0 – 15 C
Mesophiles 20 – 40 C *37C
Thermophile 50 – 90 C
Hyperthermophile > 95 C
Effects of PH Majority of microorganisms grow at a PH between 6-8
Acidophiles grow at extreme acid PH
Alkalinophiles grow at extreme alkaline PH
Osmotic pressure Most microbes exist under hypotonic or isotonic conditions 0.85% NaCl
Halophiles require a high concentration of salt
Osmotolerant or Falcultative Halopholes do not require high concentration of solute but can tolerate it when it occurs.
The Study of microbial growth 2 levels of growth
Cellular level Cell increases in size
Population Growth in population
Division of bacterial cells occur through Binary Fission
a. Organism grows b. Duplicates its genome c. Cytoplasm divides Binary Fission steps
Doubling Time how long it takes a cell to divide by BF
Growth Curve Inoculate a fresh plate/tube (media) Lag Phase(Slow growth) Log Phase Rapid division, active metabolism (Exponential Growth) Stationary Phase Nutrients are low, growth rate = death rate Death Phase Nutrients running out, death rate > growth rate
Created by: wiveys
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