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Micro 2
Chapter 7
| Question | Answer |
|---|---|
| Must be provided to an organism | Essential Nutrients |
| required in large quantities. Play principle role in cell structure and metabolism (proteins, carbs) | Macronutrients |
| – required in small amounts. Involved in enzyme function and maintenance of protein structure (manganese, zinc, nickel) | Micronutrients/Trace Elements |
| Organic Nutrients – Carbon Source, usually the products of living things | 1. Proteins 2. Lipids 3. Carbs 4. Methane (CH4) |
| – Atom or molecule that contains a combination of atoms other than carbon and hydrogen. *CO2 | Inorganic Nutrients |
| Inorganic Nutrients | 02 (Lipid synthesis an metabolism) Nitrogen (proteins, ATP, Nucleic Acids) H2O – Metabolically active Metals – Enzyme function Salts – Maintain proper osmotic pressure |
| CLASSIFICATIONS BY CARBON SOURCE | Heterotroph Autotroph |
| CLASSIFICATION BY ENERGY SOURCE | Chemotroph Phototroph |
| Chemoautotrophs | Survive totally on inorganic substances |
| Photoautotrophs | – Oxygenic Photosynthesis, Anoxygenic Photosynthesis |
| Methanogens | A kind of chemoautotroph that produces methane gas under anaerobic conditions |
| Parasites | Derive nutrients from host (Pathogens, Some are obligate parasites) |
| Transport | Movement of chemicals across the cell membrane |
| Passive Transport | does not require energy; substances exist in a gradient and move from areas of higher concentration to areas of lower concentration (Diffusion, Osmosis [Diffusion of water], Facilitated diffusion [requires a carrier] |
| Diffusion | Movement of a molecule down its concentration gradient |
| Osmosis | Diffusion of water across a semipermeable membrane |
| High solute, low solvent (water) | Hypertonic |
| Low solute, High Solvent (water) | Hypotonic |
| Isotonic | about equal parts water on both sides |
| Facilitate diffusion | Molecules diffuse down the concentration gradient through a transport protein embedded in plasma membrane |
| Active transport | requires energy and carrier proteins; gradient independent; transport is against the concentration gradient |
| Endocytosis | Eating and drinking by a cell |
| Phagocytosis | ingests substances or cells |
| Pinocytosis | ingests liquids |
| Environmental factors that influence microbes | Temperature Oxygen requirements Effects of PH Osmotic pressure |
| Psychrophiles | 0 – 15 C |
| Mesophiles | 20 – 40 C *37C |
| Thermophile | 50 – 90 C |
| Hyperthermophile | > 95 C |
| Effects of PH | Majority of microorganisms grow at a PH between 6-8 |
| Acidophiles | grow at extreme acid PH |
| Alkalinophiles | grow at extreme alkaline PH |
| Osmotic pressure | Most microbes exist under hypotonic or isotonic conditions 0.85% NaCl |
| Halophiles | require a high concentration of salt |
| Osmotolerant or Falcultative Halopholes | do not require high concentration of solute but can tolerate it when it occurs. |
| The Study of microbial growth | 2 levels of growth |
| Cellular level | Cell increases in size |
| Population | Growth in population |
| Division of bacterial cells occur through | Binary Fission |
| a. Organism grows b. Duplicates its genome c. Cytoplasm divides | Binary Fission steps |
| Doubling Time | how long it takes a cell to divide by BF |
| Growth Curve | Inoculate a fresh plate/tube (media) Lag Phase(Slow growth) Log Phase Rapid division, active metabolism (Exponential Growth) Stationary Phase Nutrients are low, growth rate = death rate Death Phase Nutrients running out, death rate > growth rate |