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Microbiology Neisser

Neisseria and Haemophilus review for Microbiology 2 Deltech Owens Campus

QuestionAnswer
What is X factor? Hemin
Hemin is ? iron containing porphyrin
What is V factor? NAD
NAD is a __ found in all living cells. coenzyme
What do you call it when bacteria grow in the vicinity of a second bacteria that produces a vital metabolite? satellitism
Which organisms studied here in this unit known to exhibit satellitism? Haemophilus influenzae. Nisseria may also do this on rare occasions.
BAP has factor __ but lacks factor __. BAP has X, lacks V
What kind of bacteria that grow on BAP also produce factor V (NAD)? Beta hemolytic species like Staph. aureus
What species is most likely exhibit satellitism around S. aureus on BAP? Haemophilus influenzae
What tests must be performed to give a presumptive ID of N. gonorrhoeae? 1. they are intracellular gram neg diplococci 2. grow on MTM in 48 hours 3. Won't grow on BAP or Choc 4. oxidase pos
N. meningitidis ferments ? glucose and maltose
N. gonorroeae ferments ? glucose
N. lactima ferments ? glucose, maltose, lactose
Moraxella catarrhalis ferments ? nothing
If a slant forms bubbles on the bottom of the tube, the organism is a (fermentor or oxidizer)? fermentor
NAD is coenzyme found in ? all living cells
Which organism forms hockey puck colonies? Moraxella catarrhalis
What organisms are in the HACEK group? 1. Aggregatibacter aprophilus 2. Aggregatibacter actinomycetemcomitans 3. Cardiobacterium hominis 4. Eikenella corrodens 5. Kingella sp.
HACEK group organisms like what kind of environment? capnophilic/CO2
Which HACEK organism pits the agar? CEK (presumably she means Cardiobacterium hominis, Eikenella corrodens, Kingella sp.)
Why do we give the Hib vaccine? To prevent infection by Haemophilus influenzae type B.
The B in Hib stands for ? B antigen on H. influenzae capsule
Which organism may appear as a "school of fish?" H. ducreyi
Which Haemophilus organism depends only on X factor (hemin)? H. ducreyi
Which Haemophilus organism depends only on factor V (NAD)? H. parainfluenzae
Which Haemophilus organism needs both factor X and V to grow? Haemophilus influenzae
Which microbial colony has a "fried egg" appearance? Mycoplasma
Which organism causes atypical/walking pneumonia? M. pneumoniae
Which organism causes Bazillian Purpuric Fever and conjuctivitis? H. aegyptius
Which organism causes chancroid in the tropics? H. chancroid
What do Cardiobacterium hominis, Eikenella corrodens, Kingella sp. all have in common? They pit the agar and are HACEK group organisms
H. ducreyi may take on what appearance when seen on a direct clinical smear? school of fish
Which gram negative diplococci is most often isolated from eye infections? Moraxella catarrhalis
Which CTA sugars should be tested to speciate a Neisseria organism? glucose, maltose, lactose, sucrose - yellow is positive
Which enzyme produces a blue color in the NET test? Beta-galactosidase
Which enzyme produces a yellow color in the NET test? Gamma-glulamyl...
Which enzyme produces a red color in the NET test but only after a PRO reagent has been added? Hydroxy...
What test can differentiate Neisseria from other cocci? Oxidase test (it's pos)
What is the most reliable test to differentiate Neisseria lactamica from Neisseria meningitidis? Lactose degradation. Both produce acid from maltose and grow on modified Thayer-Martin agar, only N lactamica ferments lactose.
Which factor does H. ducreyi need to grow? X or hemin
Mycoplasma has what appearance under a microscope? fried egg
H. aegyptius causes what diseases? Bazillian Purpuric Fever and conjuctivitis
Created by: IsaacJ
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