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Cell Biology

QuestionAnswer
Four Major Classes of Biomolecules amino acids -> proteins nucleic acids -> DNA carbohydrates -> complex carbohydrates lipids
What Allows Self-Assembly Without Aid of Temps? *Water *Molecular Properties *molecular prop *hydroph. interactions (nonpolar molecules aggregate to minimize unfavorable interactions with h20 *electrostatic interact. (dipole-dipole) *capacity to form covalent bonds *charged domains (ionic bonds) *interact. with water
Post-Translational Modifications Phosphorylated - serine, threonine, tyrosine Acetylation - lysine Methylation - lysine, arginine *proline and lysine = hydroxylated
Backbone of Polypeptides *dehydration synthesis *peptide bond is rigid and planar *alt. R groups *exposure of R groups to environ.
Sequence of a.a. Determine 3D Structure 1. hydrophob. side chains min. exposure to 2 h20 & go to interior of protein 2. charged/polar side chains are exposed (at surface of protein) to interact with h20 3. polar amide protons and carbonyl O2 form a h bond --> ahelices and bsheets
a-Helices *maximize h bonding *3.6 residues in each turn *ends of helix less stable than center *generates a dipole *P to initiate bends G for tight turns and tight packing
B-Sheets *indiv. strands form H-bonds with neighboring strands in antiparallel *anti=more stable *mostly flat but slight right-handed twist --> B-barrel
Bends * "reverse turns" or "hairpin turn" * omega turns - more loosely packed or irreg. regions * typicall end up at surface of protein * resides at the bend are gen. hydrophillic
Secondary --> Tertiary Structure * coiled -coils : 2 ahelices paired -> dimer * interactions with solvents (H20) * modular domain: indep. folded regions link up to form complex polypeptides
Nucleotides Nucleic Acids (DNA/RNA) 1.One of 4 main bases Adenine (A) Guanine (G) Cytosine (C) Thymine (T) or Uracil (U) 2. 5-Carbon sugar (deoxyribose/ribose) 3. one or more phosphate group
Nucleotides II * linked by phosphodiester bond * add of nucleotide to 3' end of sugar * all bonds are somewhat flexible
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