click below
click below
Normal Size Small Size show me how
Cell Biology
| Question | Answer |
|---|---|
| Four Major Classes of Biomolecules | amino acids -> proteins nucleic acids -> DNA carbohydrates -> complex carbohydrates lipids |
| What Allows Self-Assembly Without Aid of Temps? *Water *Molecular Properties *molecular prop | *hydroph. interactions (nonpolar molecules aggregate to minimize unfavorable interactions with h20 *electrostatic interact. (dipole-dipole) *capacity to form covalent bonds *charged domains (ionic bonds) *interact. with water |
| Post-Translational Modifications | Phosphorylated - serine, threonine, tyrosine Acetylation - lysine Methylation - lysine, arginine *proline and lysine = hydroxylated |
| Backbone of Polypeptides | *dehydration synthesis *peptide bond is rigid and planar *alt. R groups *exposure of R groups to environ. |
| Sequence of a.a. Determine 3D Structure | 1. hydrophob. side chains min. exposure to 2 h20 & go to interior of protein 2. charged/polar side chains are exposed (at surface of protein) to interact with h20 3. polar amide protons and carbonyl O2 form a h bond --> ahelices and bsheets |
| a-Helices | *maximize h bonding *3.6 residues in each turn *ends of helix less stable than center *generates a dipole *P to initiate bends G for tight turns and tight packing |
| B-Sheets | *indiv. strands form H-bonds with neighboring strands in antiparallel *anti=more stable *mostly flat but slight right-handed twist --> B-barrel |
| Bends | * "reverse turns" or "hairpin turn" * omega turns - more loosely packed or irreg. regions * typicall end up at surface of protein * resides at the bend are gen. hydrophillic |
| Secondary --> Tertiary Structure | * coiled -coils : 2 ahelices paired -> dimer * interactions with solvents (H20) * modular domain: indep. folded regions link up to form complex polypeptides |
| Nucleotides | Nucleic Acids (DNA/RNA) 1.One of 4 main bases Adenine (A) Guanine (G) Cytosine (C) Thymine (T) or Uracil (U) 2. 5-Carbon sugar (deoxyribose/ribose) 3. one or more phosphate group |
| Nucleotides II | * linked by phosphodiester bond * add of nucleotide to 3' end of sugar * all bonds are somewhat flexible |