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Sucrose Review
| Question | Answer |
|---|---|
| How many sucrose transporters are in Arabidopsis? Rice? How many share 75% identical AA? 50% identity? | 9 in Arabidopsis, 5 in rice 75% identical in 1,2, 5-9 50% identity for SUC 3-4 |
| Where are sucrose transporters located? | Plasma membrane of SE/CC |
| Sucrose 2 and 9 doesn't transport? | Sucrose, they transport glucosides ( derivatives of glucose) |
| What is the physiological role of vascular sucrose transporters? | Suc4: sucrose uptake at plasma membrane of yeast, involved in sucrose efflux from vacuole |
| How is sucrose transport at cellular level regulated? | regulated at various levels, allowing adaptation to external stimuli such as temperature, light, photoperiod, pathogen attack and other stress |
| there's a phloem specific transmembrane protein that promotes phloem loading without affecting phloem unloading, what is is called? | Tdy1 |
| Regulation of transport activity by phosphorylation? | - phosphorylation of N terminus of a sucrose transporter --> AtSUC5 - phosphorylation of serene residue at position 20 --> AtSUC1 |
| Regulation via protein- protein interactions | There are specific interactions between transporters - there is plasticity in sugar transporter activity and protein protein interactions of sucrose transporters are not restricted to plasma membrane |
| Regulation at post transcriptional level | microRNA targeted against 7 out of 9 different sucrose transporter genes have been ID-ed, except SUT2 and 4 |
| Where doe sucrose biosynthesis occur? where has it also been found? | leaf mesophyll cytoplasm, vacuoles or plastids |
| Where does the bulk of sucrose go? | SE/CC complex by proton symport in apoplasmic loading species |