Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
BIS 104 Final
Last minute important info.
| Question | Answer |
|---|---|
| Protein that prevents polymerization of ACTIN? | Thymosin |
| What energy unit does ACTIN use to polymerize? | ATP |
| What energy unit do MICROTUBULES use to polymerize? | GTP |
| What protein helps ACTIN polymerize? | Prolifin |
| What protein stabilizes MICROTUBULES so it doesn't disassemble? | MAPS (microtubule associated proteins) |
| How does GTP-gamma-S work on MICROTUBULES? | The Sulfur form of GTP cannot be hydrolyzed- so the microtubles keep conformation. DOES NOT DO THIS WITH ACTIN |
| Which filaments have polarity, which do not? | Polarity: microtubules, actin. No polarity: intermediate filaments. |
| When a vesicle is TETHERING, what two parts compliment? | Rab proteins and Rab effector proteins (tethering proteins) |
| When a vesicle is DOCKING, what two parts compliment? | V-snare (on vesicle), and T-snare (on membrane) |
| Organizations of ACTIN filament? (3) | 1. contractile bundles 2. Gel-like network 3. Tight parallel bundle |
| What protein is found in TIGHT BUNDLES? | Fimbrin |
| What protein is found in CONTRACTILE BUNDLES? | alpha-actin |
| What protein is found in LAMELLIPODIA? | Filamin |
| What protein is bind PERIPERAL MEMBRANE PROTEINS? | Spectrin |
| Caged florescence: | If marker gets further from the bacterium, then the bacterium is assembling actin closest to it. (not furthest) |
| MIcrotubulalr-dependent motor proteins: (2) | KInesin, Dyeins |
| Kinesins | motor protein dependant on microtubules. Moves toward PLUS end. Faster |
| Dyneins | motor protein dependant on microtubules. Moves toward MINUS end. Slower |
| 4 components of signal transduction: | 1. signal, 2. receptor, 3. transducer, 4. Intracellular target. |
| G-protein coupled receptors use what kind of energy? | GTP |
| Alpha-subunits of G proteins we discussed: | G-s, G-olf, G-t, G-q |
| What is the primary target of G-s? | Adenylate cyclase |
| What is the primary target of G-olf? | Adenylate cyclase |
| What is the primary target of G-q? | Phospholipase- C -B |
| What is the primary target of G-t? | Cyclic GMP Phosphodiesterase |
| What is produced in G-s? | cAMP |
| What pathway uses cAMP as second messenger? | G-s |
| What second messenger/s does G-q produce? | IP3, Diacylglycerol |
| What is necessary to activate Protein Kinase C? | Calcium, Phosphatidalserine, Diacylgycerol. |
| What is necessary to activate Na+ channels? | Cyclic GMP phosphodiesterase |
| What is necessary to activate Protein kinase A? | cAMP |
| End result of G-q pathway? | CaM-kinases, Protein kinase C |
| End result of G-t pathway? | Na+ channels |
| End result of G-olf pathway? | cAMP gated channels |
| End result of G-s pathway? | Protein Kinase A |
| What does IP3 activate? | Ca2+ and Calmodulin |
| What does Diacylglycerol activate? | Protein kinase C |
| What pathway/s involve the CREB protein? | G-s and G-olf (they also involve cAMP) |
| Signal recognized in the trans-golgi? | Mannose-6-PO4 |
| Segment of 25-30 amino acids (hydrophobic at amino terminal end: | signal to get into ER (located on NH2 end) |
| N X S/T | signal for n-glycosylation in the ER |
| KDEL | Sequence that keeps a protein in the ER- it is located on the COOH end. |
| In onder to get into the cis-golgi, a protein needs a ______ | signal patch |
| Once a protein gets into the cis-golgi, what is added? | GLcNAc |
| In the trans golgi- | The phosphoylated mannose is exposed (mannose-6-PO4) |
| mannose-6-PO4 is a signal that is added in the ______ and allows a protein to go to the _______ | Trans-golgi, Lysosome |
| If you are missing GlcNAc, you cannot make ________ | mannose-6-PO4 signal |
| In the ER, Carbohydrates are added to what amino acid? | Asperigine |
| 2 alterations that happen in the ER: | Remove signal, add carbohydrates |
| If a protein has a KDEL signal, it is destined ______ | to stay int he ER |
| If the protein does not have KDEL, and has a signal patch, the protein can move from the ____ to the ______ | ER to the cis-golgi |
| An important enzyme in the cis golgi is the _______ | GlcNAc phpsphotransferase |
| In order to get into the lysosomes the trans-golgi needs to add ______ | mannose-6-PO4 signal. |
| Signal to get into the Mitochondria: | amino-terminal amphipathic alpha-helix with positively charged residues on one face and hydrophobic residues on the other face |
| signal needs to get into the Peroxisome | -SKL at the COOH terminus of protein |
| signal needed to get into the Nucleus | 4-8 positively charged amino acids (ex: KKKRK) |
| Relax, Breathe | You will be great. |
Created by:
omygoodnessalex
Popular Biology sets