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Specimen Processing

QuestionAnswer
Compare and contrast serum and plasma. Both: translucent fluid portion of blood; rise to top of tube post cent or coag. Serum: Fluid left when blood clots; no clotting factors. Plasma: Fluid after centrifugation sans clotting; contains clotting factors.
How most anticoagulants prevent coagulation of blood. These contain preservatives that extend the metabolism & life span of RBCs. They remove/incapacitate sp elems of the clotting process(Ca+, platelet agg, thrombin, factor x).
What is a microhematocrit centrifuge used for? A clay sealed microhematocrit tube filled w/blood is balanced in this to create a PCV in order to separate elements of blood for analysis.
Serum Separator after clotting has occurred, allows for separation of cellular elements of blood from serum in order to isolate the serum in a centrifuged tube of blood.
Hemolysis Rupture of RBCs due to poor preanalytic care or physiological abnormality
Icterus Presence of excess bilirubin; causes yellow color and indicates inadequate liver function, hepatic disease, or hemolytic disorder.
Lipemia Cloudy or milky white appearance of a specimen; indicates excess lipids and/or elevated triglycerides, cholesterol or bacterial contamination
Why is plasma from an EDTA tube not used for a plasma calcium level? EDTA binds with calcium to prevent clotting; it prevents observation of calcium efficacy in the clotting process.
How does Heparin stop blood clotting? Heparin inactivates Thrombin and Factor X
5 specimens to transport in ice slurry ammonia - Green top blood gasses - syringe catecholamines - urine lactic acid -lavender(EDTA) or Green(heparin) parathyroid hormone-Lav or Lav & Red/SST
Where to collect from a patient with mastectomy or edema. Draw from arm opposite of mastectomy to avoid excess tissue fluid collection
Time sensitive tests Monitor and document time of dosage(s) and test(s)
Created by: sarahs9
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