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Phlebotomy Equipment
Phlebotomy equipment
| Question | Answer |
|---|---|
| what is the preferred anticoagulant for the latest clinical instruments, stat draws; why? | Heparin is preferred; it prevents clotting by inactivating Thrombin and Factor X |
| How does the addition of oxalates, citrates, or EDTA prevent coagulation of blood? | Oxalates, citrates, and EDTA remove calcium and prevent formation of calcium salts to prevent coagulation. |
| Which anticoagulant is preferred in preparation of blood smears; why? | EDTA prevents platelet aggregation and limits the distortion of WBCs. |
| How does the addition of heparin prevent blood from clotting? | Heparin causes the inactivation of Thrombin and Factor X. Absence of these prevents conversion of prothrombin to thrombin. |
| Why is it important to collect the correct volume of blood in the tube. | Test results may yield inappropriate results due to inappropriate concentration of blood to solution in the tube. The amount of blood may also be inadequate for a specific test. |
| The anticoagulant in Purple (lavender) topped tubes | EDTA |
| The anticoagulant in Gray-topped tubes | Potassium Oxalate & Sodium Fluoride Na2EDTA & Sodium Fluoride Sodium Fluoride only |
| The anticoagulant in Green-topped tubes | Sodium Heparin, ammonium heparin and lithium heparin |
| The anticoagulant in Light blue-topped tubes | Buffered sodium citrate (3.2% or 3.8%) |
| The anticoagulant in Red-topped tubes | NO anticoagulant or polymer |
| The anticoagulant in Speckle-topped tubes | Contains polymer barrier for serum separation |
| Which anticoagulants can be utilized in calcium determinations? | Only heparin, or lithium heparin (Green-tops) |
| Why should the gray-topped tube not be used for enzyme determinations or for hematology studies? | Gray-topped tubes contain solutions used for glycolytic inhibition tests; oxalates destroy enzymes. |
| Why should the green-topped tube not be used for blood smears? | Green-topped tubes contain heparin which causes Wright's stain to tint with a blue background. |
| What is the purpose of the gel in the mottled-topped or speckled-topped tubes? | Polymer gel in SSTs allow for separation of fluid and cells in a centrifuged sample while preventing clotting factors from acting on the RBCs. |
| Hematology uses what color-topped tube | purple (lavender)top |
| Test for trace elements uses what-color-topped tube? | royal blue top |
| chemistry tests use what color-topped tube? | Green top |
| Coagulation procedures use what color-topped tube? | Light blue top |
| Glycolytic inhibition studies use what color-topped tube? | Gray top |
| When is a syringe more appropriate for collection than a vacuum tube? | In case of fragile veins, or when a phlebotomist needs to control the amount of suction on the vein. |
| Why must the blood be allowed to clot for at least 30 minutes when obtaining serum? | Blood must clot for at least 30 min to ensure completion of formation of a fibrin clot and proper serum separation. |
| What is the usual needle gauge and length for routine collection? | Standard gauge is 21 or 22; standard length is 1 to 1.5 inches. |
| What does the gauge number of a needle indicate? | Refers to the diameter of the needle. |
| What are advantages of using the vacuum tube method? | *Most accurate & efficient *Decreased chance of spill or unnecessary needle exposure *allows for minimal discomfort to the patient *color system allows for clearly identifiable sample types |
| When is a butterfly used | small children, infants, fragile veins. They allow for a smaller angle of insertion when standard needles do not. |
| Why must the antiseptic 70% isopropyl alcohol be allowed to dry completely? | Blood contaminated with 70% isopropyl will damage blood cells and yield erroneous results. A needle stick through damp isopropyl will be painful. |
| Two substances a phlebotomist uses that should be checked for possible allergy with patient. | latex iodine |