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PCR&Electrophoresis

PCR & Electrophoresis

QuestionAnswer
What does PCR stand for? Polymerase Chain Reaction
At which temperatures is Polymerase Chain Reaction done? 95, 55, 70
What three things are added to the DNA before the first stage of PCR? DNA Polymerase, DNA Primers, Nucleotides
What happens at 95 degrees? DNA separates into 2 strands.
What happens at 55 degrees? Primers fit to the STR sequence.
What does STR stand for? Short Tandem Repeat or Satellite Repeated Sequence.
What happens at 70 degrees? DNA polymerase is attached and Nucleotides are added. The DNA is then replicated.
What is the Polymerase Chain Reaction used for? To make more DNA.
What are DNA Primers? Short sequence of DNA complimentary to the DNA adjacent to the STR.
What is the process in which the Polymerase Chain Reaction is done? Gel Electrophoresis.
What substance is the DNA put in during Gel Electrophoresis? Agarose Gel
What solution is used in Electrophoresis? Buffer solution
Which electrode does the DNA move towards? Positive
Which travel faster, small fragments of DNA or large fragments? Small fragments
Created by: JadeWarren
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