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PCR/DNA profiling

PCR

QuestionAnswer
What gets placed in the test tube at stage 1? DNA polymerase,DNA primes, nucleotides and the sample DNA.
What temperatures are used during PCR? 95, 70 and 55.
What happens in stage 1? The DNA separates into two strands, denaturation.
What happens in stage 2? DNA primers attach at the start of the STR.
What happens in stage 3? DNA polymerase attach, nucleotides are added and the STR and DNA are replicated.
What is the name of the length of DNA between exons? Introns
What can be known as the 'expressed region' or gene? Exons
What does STR stand for? Short tandem repeat
What is the name of the enzyme that breaks the hydrogen bond? Helicase
What does PCR stand for? Polymerase chain reaction
What is the name of the enzyme DNA is treated with? Restriction enzymes or restriction endonucleases.
Why is DNA profiling used? To be able to identify a person by comparing their DNA.
Where is DNA obtained? From white blood cells, bone marrow or sperm.
What does the restriction enzyme do? The enzyme cuts the DNA at a specific base, where an intron is repeating the sequence.
How can DNA be preserved? Using liquid nitrogen to freeze it.
Where do restriction enzymes come from? Bacteria.
What is the name of the position on a chromosome were a gene is? Locus.
What is the name of the stage of PCR where DNA is copied? Extension
What is the name of the stage of PCR where DNA is separated? Denaturation.
What is the name of the stage of PCR where primers attach to DNA? Annealing.
Created by: rebeccawilson
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