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PCR & DNA Profiling

PCR

QuestionAnswer
What does PCR stand for ? Polymerase Chain Reaction
What is added to the DNA sample during PCR ? DNA poymerase, DNA primer (florescent), nucleotides
What is the temperature used to break the DNA into 2 strands ? 95 degrees Celsius
What is the primer used for ? The primer bonds to the DNA to form a starting point for DNA synthesis
What is a restriction enzyme ? An enzyme used to cut the STR
which end of the DNA strand do the primers bond to ? The 3 end
Where does the DNA polymerase come from for PCR ? Bacteria from hot water geysers
Define STR Short tandem repeat
why can't you use human polymerase during PCR ? The Enzyme will be denatured
What is PCR used for ? Amplifying DNA
What is another name for DNA profiling ? DNA fingerprinting, Gel electrophoresis
what is the name of the jelly where the DNA samples are put into ? Agarose jelly
What is the name of the holes made in the agarose for the DNA samples ? Wells
What is the buffer liquid used for in gel electrophoresis ? To prevent any change in pH
What charge does DNA have ? Negative
When DNA probs are added top the DNA how will you be able to see them ? By adding a florescent or radioactive substance to the DNA probs
Which DNA strands move fastest ? Short strands with the smallest number of base pairs
When do the DNA strands separate ? when the DNA is attached to the nylon paper
Created by: gemacey
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