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DNA profiling

PCR and DNA profiling

QuestionAnswer
Name the temperatures that DNA is treated at in PCR 95, 55 and 70 degrees celsius
What charge is DNA? negative
Name the jelly used in gel electrophoresis agarose
What does the distance the DNA has travelled, depend on? the number of base pairs in DNA length
What does STR stand for? Short tandem repeat/ satellites
non coding blocks introns
coding blocks exons
what do the DNA strands have to do in order for primers to attach in Southern blotting DNA strands must separate
Why is PCR carried out? To increase the quantity of DNA in the sample
another name for DNA fingerprinting gel electrophoresis/ DNA profiling
why are DNA probes used in Southern blotting to make the DNA show up
name the liquid in gel electrophoresis buffer solution
name the types of DNA probe used fluorescent/radioactive
what is used to cut DNA? restriction enzymes
other than the DNA sample what is added in PCR? DNA polymerase, DNA primers and nucleotides
where does the DNA polymerase come from? taq amylase (bacteria)
name the scientist who developed the PCR method Alec Jeffreys
why would you create a DNA profile? crime, paternity, identification, research, food standards
what are short tandem repeats? short DNA sequences that are found in introns
How many base pairs can form an STR? 2-50
How many times is the sequence in an STR repeated? 5-100s
Is it possible for two people to have the same number of repeats? YES!
where do restriction enzymes cut? wherever on the DNA the scientist wishes them to be cut (either side of an STR)
in gel electrophoresis what are the holes called that the DNA is inserted into? wells
At a particular loci would the number of STR repeats be the same on both chromosomes of a homologous pair? No, it is likely that the number of repeats would be different on the paternal strand to the maternal strand
Created by: 07blandforderin
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