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Biotech I Lab key
| Term | Definition |
|---|---|
| CODIS | Combined DNA Index System - the DNA file maintained by the Federal Bureau of Investigation |
| RFLP | In molecular biology, restriction fragment length polymorphism, or RFLP (commonly pronounced “rif-lip”), is a technique that exploits variations in homologous DNA sequences. |
| STICKY ENDS | refers to the properties of the end of a molecule of DNA or a recombinant DNA molecule |
| DNA FINGERPRINTING | A molecular genetic method that enables identification of individuals using hair, blood, semon or other biological samples, based on unique patterns (polymorphisms) in their DNA. |
| CLEAVE | The state of being split or cleft; a fissure or division. |
| DNA | A genetic protein like nucleic acid on plant and animal genes and chromosomes that controls inheritance. |
| COMB | Center of Marine Biology |
| RESTRICTION ENZYME | is a type of enzyme which have the ability to "cut" molecules of DNA. |
| EXCLUSION | The act of expelling or ejecting a foetus or an egg from the womb. |
| HYBRIDIZATION | The production of offspring from two parents (such as plants, animals, or cells) of different breeds, species, or varieties. |
| VNTR | A Variable Number Tandem Repeat (or VNTR) is a location in a genome where a short nucleotide sequence is organized as a tandem repeat. |
| NUCLEOTIDES | A unit of DNA made up of a sugar molecule, a phosphate molecule, and a nitrogen molecule containing chemicals called bases. |
| GEL LANEES | If several samples have been loaded into adjacent wells in the gel, they will run parallel in individual lanes |
| DNA FRAGMENTS | fragments that, when exposed to photographic film, reveal an image of the DNA. |
| PALINDROME | is a nucleic acid sequence (DNA or RNA) that is the same whether read 5' (five-prime) to 3' (three prime) on one strand or 5' to 3' on the complementary strand with which it forms a double helix. |
| WELLS | By placing the molecules in wells in the gel and applying an electric field, the molecules will move through the matrix at different rates, determined largely by their mass when the charge to mass ratio (Z) of all species is uniform. |
| STR | are short sequences of DNA, normally of length 2-5 base pairs, that are repeated numerous times in a head-tail manner, i.e. the 16 bp sequence of "gatagatagatagata" would represent 4 head-tail copies of the tetramer "gata". |
| ELECTRIC FIELD | a property of a patch of space which causes the acceleration of electric charges located at that patch of space. |
| INCLUSION | are nuclear or cytoplasmic aggregates of stainable substances, usually proteins. They typically represent sites of viral multiplication in a bacterium or a eukaryotic cell and usually consist of viral capsid proteins. |
| MICROLITER | one millionth of a lite |
| HUMAN GENOME | is the complete set of genetic information for humans (Homo sapiens). This information is located as DNA sequences within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. |
| BLUNT CUT | Enzymes that cut at precisely opposite sites in the two strands of DNA generate blunt ends without overhangs. SmaI is an example of an enzyme that generates blunt ends. |
| BUFFER | are formulated based on standard protocols, feature high-quality raw materials, and may be used in various immunoassays, including immunofluorescence, IHC, ELISA, and WB |
| RECOMBINANT DNA | molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological organisms. |
| DNA POLYMERASE | is a cellular or viral polymerase enzyme that synthesizes DNA molecules from their nucleotide building blocks. |
| GENE | is a molecular unit of heredity of a living organism. |
| DIGITAL MICROPIPET | A pipette designed for the measurement of very small volumes. |
| STAGGERED CUT | The cleavage of two opposite strands of duplex DNA at points near one another. |
| AGAROSE GEL | is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose |
| INCONCLUSIVE | not leading to a firm conclusion; not ending doubt or dispute on an experiment. |
| FINGERPRINT | its narrow sense is an impression left by the friction ridges of a human finger. |
| LUMINOL | is a versatile chemical that exhibits luminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent. |
| PCR | polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. |
| RESTRICTION SITE | A site or area that is restricted from inexperienced people. |
| GEL ELECTROPHORESIS | s a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. |
| EVIDENCE | Basically the proof of the investigation to see if the truth is revealed. |
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