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Micro 3
Test 3 (Volume 1)
| Question | Answer |
|---|---|
| What does Bergey's Manual of Systematic Bacteriology study? | The relation between microorganisms. |
| How many volumes are found in BMSB? | Five volumes |
| What is the first volume called? | Domain Bacteria and Domain Bacteria |
| What is special about volume one? | It is the most ancient. |
| What is Domain Archaea referred to as? | The "Other" prokaryotic domain |
| What are the three ways Archaea differ from bacteria? | 1) Ribosomal RNA sequences are different 2) Cell wall composition lacks peptidoglycan 3)Cell membrane lipids have branched hydrocarbon chains |
| What type of conditions do most Archaea live? | In moderate environmental conditions, but many are extremophiles too. |
| How many types of Archaea cause disease? | None. |
| What are the 4 Phyla of Domain Archaea? | 1) Crenarchaeota 2) Euryarchaeota 3) Korarhcaeota 4) Nanoarchaeota |
| What is a characteristic of Phyla Crenarchaeota? | Acidic, high temps |
| What types of organism like to live in Phyla Euryarchaeota? | Salt lovers, methane producers |
| What type of organisms are known to live in Phyla Nanoarchaeota? What is special about this phyla? | Tiny organisms! Newly proposed phyla. |
| What are extremophiles? | Organisms that REQUIRE extreme conditions of temperature, pH, and/or salinity |
| What happens when thermophiles are not maintained at their high temps? | DNA/RNA, cytoplasmic membranes, and proteins do not function. |
| What temperature do hypothermophiles require? | Higher than 80 degrees. |
| Where can hypothermophiles live well? | Acidic hot springs, deep ocean rifts, and similar terrestrial volcanoes |
| Where does the extremophile Pyrodictium live? What temperature does it live best at? What structure does it have that is special? | In deep sea hydrothermal vents; @85 degrees; Protein tubules- attach to grains of sulfur which is used as a final electron acceptor in aerobic respiration |
| What are halophiles? | Extremophiles that require a concentration of NaCl greater than 9% to maintain integrity of cell walls. Between 17-23% for growth and reproduction. |
| What are 3 good saline habitats for halophiles? | The dead sea, Great salt lake, Solar evaporation ponds |
| How do halophiles live in such salinity? | Utilizies compatible solutes (KCl) and encodes acidic protein to prevent protein precipitation |
| What two pigments do halophiles contain that protects them from intensive visible and UV light? | Red and orange pigments |
| What are the purpose of the red and orange pigments in halophiles? | Play a role in protecting them from intense visible and UV light |
| What is a specific organism in the halophile group that we studied? | Halobacterium salinarium |
| Is Halobacterium salinarium chemoauto/chemohetero/photoauto/photohetero? What do these synthesize in the cell membrane? | Photoheterotroph; Synthesize purple proteins in cell membrane called bacteriorhodopsins |
| What is another type of group in Archaea that convert C02, H2, and organic acids into methane gas? | Methanogens |
| Are methanogens chemoautotrophic or chemoheterotophic? | Chemoautotrophic |
| What is an industrial use for methanogens? | Sewage treatments in the digestion of sludge |
| Where are methanogens found? | Freshwater, hypersaline environments, sediments, animal intestines |
| What temperatures, pHs, and salinity are best for methanogens? | A wide range of all! At least one halophilic genus is known |
| What are 3 examples of methanogens and what characteristics do they have? | 1) Methanopyrus kandleri- optimum temp 80-100 2) Methanosarcina barkeri- produces SOD, lives in cow rumen 3)Methanospirillium hungatei-anaerobic, often found in sewage sludge |
| What are the rRNA sequences and growth sequences of Domain Bacteria similar to? | Earliest bacteria |
| Where are organisms of Domain Bacteria found? | Hot, acidic, anaerobic |
| What is the oldest living fossil on the earth today known as? | Aquifex aeolicus |
| What are some characteristics of Aquifex aeolicus? | Hyperthermophilic, chemoautotrophic, rod shaped, flagellated, found in hot springs or near volcanoes |
| What is special about the gene sequence of Aquifex aeolicus? | It has the smallest gene sequences, 16% of sequence is from archaea |
| Is Aquifex aeolicus aerobic or anaerobic? | Aerobic, but in the absence of oxygen, can reduce nitrogen for nutrients |
| Which bacteria is known as Conan the Bacteria? | Deinococcus radiodurans |
| Why is Deinococcus known as Conan? | toughest bacteria on the planet- has gram positive cocci with outer membrane similar to gram negative- extremely resistant to radiation(because of manganese-protects DNA)- lives in wide range of environments |
| What is a major division of Domain Bacteria? | Phototrophic Bacteria |
| What is a phototroph? | acquires energy for anabolism by absorbing light with pigments located in thylakoid membranes |
| What are the 4 groups of phototrophic bacteria? | 1) Blue green bacteria (cyanobacteria) 2) Green and purple sulfur bacteria 3) Green and purple non-sulfur bacteria 4) Heliobacteria |
| Is Cyanobacteria gram positive or gram negative? | gram negative |
| What is special about the filament of the cyanobacteria? | It is surrounded by a gelatinous glycocalyx sheath |
| What is a filament? | Chain of cells |
| What is the main purpose of an akinete? | Reproduction! |
| What is the function of the heterocyst? | To fix nitrogen when no oxygen is available |
| What is special about the photosynthesis of the cyanobacteria? | Thought to have transferred early earth into our oxygen containing version |
| What are stromatolites? | Living fossils, made of CaCO3, 3.5 billion years old, fresh and salt water living |
| Are green and purple bacteria oxygenic or anoxygenic? | Anoxygenic --> use bacteriochlorophylls and photosystem 1 |
| Are SULFUR green and purple bacteria photoautotrophic or photoheterotrophic? | Photoautotrophic |
| What do the purple bacteria produce? | sulfur granules |
| What is an example of a proteobacteria? | Chromatium okenii |
| Are NON-SULFUR green and purple bacteria photoautotrophic or photoheterotrophic? | Photoheterotrophic |
| What do the green bacteria do? | Excrete sulfur |
| What is an example of nonsulfur green and purple bacteria? | Cloroflexus aggregans |
| What are some characteristics of Heliobacteria? | photoheterotroph, anaerobic, nitrogen fixers, use bacteriochlorophyll |