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Chapter 7

Microbial Growth

TermDefinition
acidophile microorganism that has its optimum growth between pH values 0 and about 5.5
aerobe organism that grows in the presence of atmospheric oxygen
aerotolerant anaerobe microbe that grows equally well whether or not oxygen is present
alkaliphile (alkalophile) microorganism that grows best at pH values about 8.5-11.5
anaerobe organism that can grow in absence of free oxygen
batch culture population of microorganisms growing in closed culture vessel containing single batch of medium
biofilm organized microbial communities consisting of layers of cells associated with surfaces and surrounded by an extracellular polymeric matrix
chemostat a continuous culture apparatus that feeds medium into the culture vessel at the same rate as medium containing microorganisms is removed; the medium contains a limiting quantity of one essential nutrient
colony forming units (CFU) number of microorganisms that form colonies when cultured using spread plates or pour plates; used as a measure of the number of viable microorganisms in a sample
cytokinesis processes that apportion the intracellular contents, synthesize a septum, and divide a cell into two daughter cells during cell division
exponential (log) phase phase of growth curve during which the microbial population is growing at a constant and maximum rate, dividing and doubling at regular intervals
extremophiles microorganisms that grow under harsh or extreme environments
facultative anaerobe microorganism that does not require oxygen for growth but grows better in its presenxe
generation (doubling) time time required for a microbial population to double in number
halophile microorganism that requires high levels of sodium chloride for growth
hyperthermophile a bacterium or archaeon with a growth optimum above 85 degrees Celsius
lag phase period following the introduction of microorganisms into fresh batch culture medium when there is no increase in cell numbers of mass
mesophile microorganism growth optimum temperature around 20-45 Celsius
microaerophile microorganism that requires low levels of oxygen for growth (2-10%) but is damaged by normal atmospheric oxygen levels
most probable number (MPN) method for determining number of viable cells in a liquid sample. Samples are incubated in a suitable liquid medium in dilution series, the most dilute sample to show growth is between 1-10 cells
neutrophile microorgnism that grows best at neutral pH range between 5.5-8.0
obligate aerobe organism that grows only when oxygen is present
obligate anaerobe organism that grows only when oxygen is absent
osmotolerant organisms that grow over a wide range of water activity or solute concentration
psychrophile microorganism that grows well at 0 degrees C, optimum growth temperature of 15 degrees C or lower
quorum sensing exchange of extracellular molecules that allows microbial cells to sense cell density
stationary phase phase of microbial growth in batch culture when population growth ceases and the growth curve levels off
thermophile microorganism that can grow at temperatures 55 degrees Celsius or higher
water activity (aw) quantitative measure of water availability in a habitat
Created by: 100002522426124
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